An Alternative Method for the Simultaneous Determination of Copper and Lead for Quality Control of Sugar Cane Spirit using a Nanotube-Based Sensor

The development of an electroanalytical method for simultaneous determination of copper and lead ions in sugar cane spirit (cachaca) using carbon paste electrode modified with ascorbic acid and carbon nanotubes (CPE-AaCNT) is described. Squarewave voltammetry (SWV) with anodic stripping was employed, and this technique was optimized with respect to the following parameters: frequency (50 Hz), amplitude (100 mV) and scan increment (9 mV). The analytical curves were linear in the range from 0.0900 to 7.00 mg L-1 for lead and copper. The limits of detection were 48.5 and 23.9 mu g L-1 for lead and copper, respectively. The developed method was applied to the simultaneous determination of copper and lead in five commercial samples of sugar cane spirit. The results were in good agreement with those obtained by F AAS/GF AAS (flame atomic absorption spectrometry/graphite furnace atomic absorption spectrometry) and showed that CPE-AaCNT can be successfully employed in the simultaneous determination of these metals in real sugar cane spirit samples.Universidade Federal de Vicosa (UFV)Universidade Federal de Vicosa (UFV)Universidade Federal de Lavras (UFLA)Universidade Federal de Lavras (UFLA)Universidade de Sao Paulo (USP)Universidade de Sao Paulo (USP)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP

Safety of Immunosuppressive Drugs Used as Maintenance Therapy in Kidney Transplantation: A Systematic Review and Meta-Analysis

To evaluate the safety of regimens containing calcineurin inhibitors (CNI), proliferation signal inhibitors (TOR-I) and antimetabolites, we conducted a meta-analysis of randomized clinical trials (RCTs) and observational studies. A total of 4,960 citations were identified in our electronic search and 14 additional articles were identified through hand searching. Forty-eight articles (11,432 participants) from 42 studies (38 RCTs and four cohorts) met the inclusion criteria. Meta-analysis results revealed the following: (i) tacrolimus was associated with an increased risk for diabetes and lower risk of dyslipidemia, compared to cyclosporine; (ii) mycophenolate mofetil (MMF) was associated with increased risk for total infections, abdominal pain, diarrhea and vomiting, compared with azathioprine; (iii) sirolimus was associated with higher risk of anemia, diabetes, dyslipidemia, lymphoceles and withdrawal compared to tacrolimus or cyclosporine, and cyclosporine was associated with an increased risk of CMV infection; (iv) the combination of CNI with antimetabolites was associated with more adverse events than CNI alone; (v) TOR-I was related to more adverse events than MMF. The data observed in this meta-analysis are similar to those describe by others authors; thus, the choice of treatment must be made by the clinical staff based on specific patient characteristics

Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9

We have evaluated RECK (reversion-inducing-cysteine-rich protein with Kazal motifs), MMP-2 (matrix metalloproteinase-2), MMP-3, and MMP-9 involvement during palate development in mice by using various techniques. Immunohistochemical features revealed the distribution of RECK, MMP-2, and MMP-3 in the mesenchymal tissue and in the midline epithelial seam at embryonic day 13 (E13), MMPs-2, -3, and -9 being particularly expressed at E14 and E14.5. In contrast, RECK was weakly immunostained at these times. Involvement of MMPs was validated by measuring not only their protein expression, but also their activity (zymograms). In situ hybridization signal (ISH) for RECK transcript was distributed in mesenchymal and epithelial regions within palatal shelves at all periods evaluated. Importantly, the results from ISH analysis were in accord with those obtained by real-time polymerase chain reaction. The expression of RECK was found to be temporally regulated, which suggested possible roles in palatal ontogeny. Taken together, our results clearly show that remodeling of the extracellular matrix is finely modulated during secondary palate development and occurs in a sequential manner.Universidade de São Paulo - Pró-Reitoria de Pesquisa PRP-USPUniversidade de São Paulo - Pró-Reitoria de Pesquisa PRP-USPCAPESCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP[01/10707-7]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP[08/53003-9]CNPq[350084/03-3]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPq[475721/2003-9]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPq[505350/04-1]Financiadora de Estudos e Projetos (FINEP)FINEP[01.04.0469.00

Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9

We have evaluated RECK (reversion-inducing-cysteine-rich protein with Kazal motifs), MMP-2 (matrix metalloproteinase-2), MMP-3, and MMP-9 involvement during palate development in mice by using various techniques. Immunohistochemical features revealed the distribution of RECK, MMP-2, and MMP-3 in the mesenchymal tissue and in the midline epithelial seam at embryonic day 13 (E13), MMPs-2, -3, and -9 being particularly expressed at E14 and E14.5. In contrast, RECK was weakly immunostained at these times. Involvement of MMPs was validated by measuring not only their protein expression, but also their activity (zymograms). In situ hybridization signal (ISH) for RECK transcript was distributed in mesenchymal and epithelial regions within palatal shelves at all periods evaluated. Importantly, the results from ISH analysis were in accord with those obtained by real-time polymerase chain reaction. The expression of RECK was found to be temporally regulated, which suggested possible roles in palatal ontogeny. Taken together, our results clearly show that remodeling of the extracellular matrix is finely modulated during secondary palate development and occurs in a sequential manner3406169CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFINANCIADORA DE ESTUDOS E PROJETOS - FINEPFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP350084/03-3; 475721/2003-9; 505350/04-1sem informação01.04.0469.0001/10707-7; 08/53003-

Os direitos da mulher à terra e os movimentos sociais rurais na reforma agrária brasileira Women's land rights and rural social movements in the Brazilian agrarian reform

Este artigo examina a evolução da reivindicação dos direitos da mulher à terra na reforma agrária brasileira sob o prisma dos três principais movimentos sociais rurais: o Movimento dos Trabalhadores Rurais Sem Terra (MST), os sindicatos rurais e o movimento autônomo de mulheres rurais. O mérito maior por levantar a questão dos direitos da mulher à terra é das mulheres dentro dos sindicatos rurais. Os direitos formais das mulheres à terra foram conseguidos na reforma constitucional de 1988, e em grande medida isso foi um subproduto do esforço para acabar com a discriminação contra as mulheres em todos as suas dimensões. A conquista das igualdades formais, contudo, não levou a um aumento na parcela de mulheres beneficiárias da reforma, a qual permaneceu baixa até a metade da década de 1990. Isso aconteceu principalmente porque garantir na prática os direitos da mulher à terra não estava entre as prioridades dos movimentos sociais rurais. Além disso, o principal movimento social a determinar o passo da reforma agrária, o (MST), considerava classe e gênero questões incompatíveis. Próximo ao final da década de 1990, entretanto, havia uma consciência crescente de que deixar de reconhecer os direitos da mulher à terra era prejudicial ao desenvolvimento e à consolidação dos assentamentos da reforma agrária e, portanto, para o movimento. O crescente consenso, entre todos os movimentos sociais rurais, sobre a importância em assegurar o direito da mulher à terra, junto com um lobby efetivo, encorajou o Estado em 2001 a adotar mecanismos específicos para a inclusão de mulheres na reforma agrária.<br>This article examines the evolution of the demand for women's land rights in the Brazilian agrarian reform through the prism of the three main rural social movements: the landless movement, the rural unions and the autonomous rural women's movement. Most of the credit for raising the issue of women's land rights rests with women within the rural unions. That women's formal land rights were attained in the constitucional reform of 1988 was largely a by-product of the effort to end discrimination against women in all it dimensions. The achievement of formal equality in land rights, nonetheless, did not lead to increases in the share of female beneficiaries of the reform, which remained low in the mid-1990s. This was largely because securing women's land rights in practice was not a top priority of any of the rural social movements. Moreover, the main social movement determining the pace of the agrarian reform, the land-less movement, considered class and gender issues to be incompatible. By the late 1990s, nonetheless, there was growing awareness that failure to recognize women's land rights was prejudicial to the development and consolidation of the agrarian reform settlements and thus the movement.The growing consensus among all the rural social movements of the importance of securing women's land rights, coupled with effective lobbying, encouraged the State in 2001 to adopt specific mechanisms for the inclusion of women in the agrarian reform