7 research outputs found
Nucleolar Organizer Region (nors) In Pseudocarcinomatous Hyperplasia And Squamous Cell Carcinoma Of The Oral Mucosa.
Counts of nucleolar regions (NORs) demonstrated by a silver staining technique in paraffin sections, have been used to distinguish benign from malignant lesions. AgNORs were studied in 24 biopsies from oral cavity (5 cases of normal oral mucosa, 5 of pseudocarcinomatous hyperplasia and 14 of squamous cell carcinoma, subdivided according to degree of differentiation: 5 grade 1, 5 grade 2 and 4 grade 3) to find whether they were helpful in distinguishing pseudocarcinomatous hyperplasia due to chronic parasitic infections from squamous cell carcinoma. Two methods of counting AgNORs were used: (A) a simpler one which counts nucleolar clusters (AgNU) and satellite AgNORs and the other (B) counting all individual AgNORs, including those within AgNUs. In both methods the lowest mean values were observed for grade 3 carcinoma, while the highest belonged to grade 2 carcinoma. The simpler method (A) was the most useful because AgNU counts showed significant difference when pseudocarcinomatous hyperplasia was compared with grade 1 and 2 carcinomas, which are the most difficult to discriminate from it. However the overlapping of values render the technique of limited use in individual cases.113169369
Morphometry And Histology Of Gonads From 13 Children With Dysgenetic Male Pseudohermaphroditism
Background. - Dysgenetic male pseudohermaphroditism (DMP) is a sexual differentiation disorder characterized by bilateral dysgenetic testes, persistent müllerian structures, and cryptorchidism in individuals with a 46,XY karyotype. However, the histologic criteria for the diagnosis of DMP are poorly established. Objective. - To determine gonadal histology in children with DMP. Patients and Methods. - Between 1996 and 1998, 13 patients with DMP were evaluated on our service. The clinical diagnosis of DMP was based on a 46,XY karyotype, sex ambiguity, high levels of follicle-stimulating hormone and low levels Of antimüllerian hormone, a decreased testosterone response to human chorionic gonadotropin stimulation without accumulation of testosterone precursors, and the presence of müllerian structures. Molecular sequencing the HMGbox region of the SRY gene did not reveal any mutations. Biopsies were performed for 22 of 26 gonads (patient age at the time of biopsy, 16 months to 10 years). Conventional microscopy was used to evaluate mean tubular diameter, tubular fertility index, and number of Sertoli cells per tubular profile. Results. - All 26 gonads were located outside of the labioscrotal folds. Their histologic features varied from only a reduction in tubular size to features of a streak gonad. Five of the 22 gonads grossly resembled a streak gonad. The mean tubular diameter was severely reduced (>30% reduction relative to the normal tubular diameter for the patient's age) in 4 gonads, markedly reduced (10%-30%) in 11 gonads, slightly reduced (<10%) in one gonad, and normal in one gonad. The tubular fertililty index, expressed as the percentage of tubular profiles containing germ cells, was severely reduced (<30% of normal values) in 9 gonads, markedly reduced (50%-30%) in 2 gonads, and normal in 6 gonads. The number of Sertoli cells per tubular profile was elevated in 16 gonads and normal in one gonad. Thin tubules surrounded by fibrous tissue were occasionally observed. Conclusion. - The histologic findings confirmed the clinical diagnosis of DMP in every patient in the present series. 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Downregulation Of 14q32 Micrornas In Primary Human Desmoplastic Medulloblastoma
Medulloblastoma (MB) is one of the most common pediatric cancers, likely originating from abnormal development of cerebellar progenitor neurons. MicroRNA (miRNA) has been shown to play an important role in the development of the central nervous system. Microarray analysis was used to investigate miRNA expression in desmoplastic MB from patients diagnosed at a young age (1 or 2 years old). Normal fetal or newborn cerebellum was used as control. A total of 84 differentially expressed miRNAs (64 downregulated and 20 upregulated) were found. Most downregulated miRNAs (32/64) were found to belong to the cluster of miRNAs at the 14q32 locus, suggesting that this miRNA locus is regulated as a module in MB. Possible mechanisms of 14q32 miRNAs downregulation were investigated by the analysis of publicly available gene expression data sets. First, expression of estrogen-related receptor-γ (ESRRG), a reported positive transcriptional regulator of some 14q32 miRNAs, was found downregulated in desmoplastic MB. Second, expression of the parentally imprinted gene MEG3 was lower in MB in comparison to normal cerebellum, suggesting a possible epigenetic silencing of the 14q32 locus. miR-129-5p (11p11.2/7q32.1), miR-206 (6p12.2), and miR-323-3p (14q32.2), were chosen for functional studies in DAOY cells. Overexpression of miR-129-5p using mimics decreased DAOY proliferation. 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Granulocytic Sarcoma Of The Larynx Preceding Chronic Myeloid Leukemia
The authors report one case of granulocytic sarcoma infiltrating the larynx and cervical lymph nodes in a 50-year-old smoking patient. At the time of diagnosis there was no clinical and laboratory evidence of acute myeloid leukemia or chronic myeloproliferative disease. Four months after diagnosis, bone marrow morphology was consistent with chronic myeloid leukemia, accelerated phase. Cytogenetic abnormalities (Ph 1 chromosome, t (1; 12)(p36; p13), and trisomy of chromosome 20) were also found in hemopoetic cells. Granulocytic sarcoma preceding installation of chronic myeloid leukemia, as described here, seems to be a rare clinical event.18991084108
Renal Medullary Carcinoma: Report Of Seven Cases From Brazil
We report seven cases of renal medullary carcinoma collected from several institutions in Brazil. In spite of a relatively high incidence of sickle cell trait in Brazil, this is a rare tumor. All patients were males between the ages of 8 and 69 years (mean 22 years). From the collected information, the most frequent presenting symptoms were gross hematuria and flank or abdominal pain. The duration of symptoms ranged from 1 week to 5 months. Most of the tumors were poorly circumscribed arising centrally in the renal medulla. Size ranged from 4 to 12 cm (mean 7 cm) and hemorrhage and necrosis were common findings. All seven cases described showed sickled red blood cells in the tissue and six patients were confirmed to have sickle cell trait. All cases disclosed the characteristic reticular pattern consisting of tumor cell aggregates forming spaces of varied size, reminiscent of yolk sac testicular tumors of reticular type. Other findings included microcystic, tubular, trabecular, solid and adenoid-cystic patterns, rhabdoid-like cells and stromal desmoplasia. A peculiar feature was suppurative necrosis typically resembling microabscesses within epithelial aggregates. The medullary carcinoma of the 69-year-old patient was associated with a conventional clear cell carcinoma. To our knowledge, this association has not been previously reported and the patient is the oldest in the literature. The survival after diagnosis or admission ranged from 4 days to 9 months. The 8-year-old African-Brazilian patient with a circumscribed mass is alive and free of recurrence 8 years after diagnosis. This case raises the question whether a periodic search for renal medullary carcinoma in young patients who have known abnormalities of the hemoglobin gene and hematuria could result in an early diagnosis and a better survival. © 2007 USCAP, Inc All rights reserved.209914920Davis Jr, C.J., Mostofi, F.K., Sesterhenn, I.A., Renal medullary carcinoma. The seventh sickle cell nephropathy (1995) Am J Surg Pathol, 19, pp. 1-11Leitao, V.A., Silva Jr, W., Ferreira, U., Renal medullary carcinoma. Case report and review of the literature (2006) Urol Int, 77, pp. 184-186Hsu, S.M., Raine, L., Fanger, H., Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC labeled antibody (PAP) procedures (1981) J Histochem Cytochem, 29, pp. 557-589Berman, L.B., Sickle cell nephropathy (1974) JAMA, 228, p. 1279Beutler, E., Disorders of hemoglobin structure: Sickle cell anemia and related abnormalities (2006) Williams Hematology, pp. 667-700. , Lichtman MA, Beutler E, Kipps TJ, Seligsohn U, Kaushansky K, Prchal JT eds, 7th edn. McGraw-Hill: New-YorkKhan, A., Thomas, N., Costello, B., Renal medullary carcinoma: Sonographic, computed tomography, magnetic resonance and angiographic findings (2000) Eur J Radiol, 35, pp. 1-7Simpson, L., He, X., Pins, M., Renal medullary carcinoma and ABL gene amplification (2005) J Urol, 173, pp. 1883-1888Chapadeiro E, Maciel R, Jamra M, et al. Linfonodos, baço, medula óssea e sangue. Tumores do Sistema Hemolinfático. Timo. In: Lopes ER, Chapadeiro E, Raso P, Tafuri WL (eds). Bogliolo Patologia, 4th edn. Guanabara Koogan: Rio de Janeiro, 1987, pp 651-652Davis Jr, C.J., Renal medullary carcinoma (2004) World Health Organization Classification of Tumours, Pathology and Genetics of Tumors of the Urinary System and Male Genital Organs, pp. 162-192. , Eble JN, Sauter G, Epstein JI, et al eds, IARC Press: LyonSwartz, M.A., Karth, J., Schneider, D.T., Renal medullary carcinoma: Clinical, pathologic, immunohistochemical, and genetic analysis with pathogenetic implications (2002) Ped Urol, 60, pp. 1083-1089Rodriguez-Jurado, R., Gonzalez-Crussi, F., Renal medullary carcinoma (1996) J Urol Pathol, 4, pp. 191-203Srigley, J.R., Eble, J.N., Collecting duct carcinoma of kidney (1998) Semin Diagn Pathol, 15, pp. 54-67Kennedy, S.M., Merino, M.J., Linehan, W.M., Collecting duct carcinoma of the kidney (1990) Hum Pathol, 21, pp. 449-456Amin, M.B., Varma, M.D., Tickoo, S.K., Collecting duct carcinoma of the kidney (1997) Adv Anat Pathol, 4, pp. 85-94Yang, X.J., Sugimura, J., Tretiakova, M.S., Gene expression profiling of renal medullary carcinoma: Potential clinical relevance (2004) Cancer, 100, pp. 976-985Figenshau, R.S., Basler, J.W., Ritter, J.H., Renal medullary carcinoma (1998) J Urol, 159, pp. 711-713Pisani, P., Bray, F., Parkin, D.M., Estimates of the worldwide prevalence of cancer for 25 sites in the adult population (2002) Int J Cancer, 97, pp. 72-8