3 research outputs found
A targeted metabolomics method for the rapid quantification of multiple classes of phenolics in the fruits of Rosaceae
In recent years, the interest in phenolic compounds has been increasing due to
compelling evidences of their beneficial health properties and to their impact on food quality. The complexity and remarkable diversity of phenolics has challenged the
analytical performances of separation and detection methods in terms of resolving
power, selectivity and sensitivity for the identification and quantification of these
compounds in different matrices. Targeted metabolomics is a strategy based on the use
of predefined metaboliteāspecific signals, such as MRM transitions, that can be used to
accurately determine the concentrations of a wide range of known metabolites. We developed a rapid and versatile UPLCāMS/MS based method for the quantification of
>150 phenolics, such as benzoates, phenylpropanoids, coumarins, stilbenes,
dihydrochalcones and flavonoids in fruits. Compounds commonly occurring in plants
were included in the method together with metabolites specific of a single species or
family. Reverseāphase chromatography was optimised to achieve separation of the
compounds over 15 min, reducing possible ion suppression effects and resolving many
isomeric compounds. The optimal fragmentation conditions for each analyte were studied and MRM transitions were selected for accurate quantification. The
effectiveness of the method was validated by studying the limits of detection and
quantification, the linearity ranges of the instrumental response and the repeatability of the analysis. The method was successfully applied and validated for the analysis of apples, cherries, raspberries, strawberries, as well as grape, wine and green tea, and was shown to represent a valuable tool for the quantitative evaluation of the chemical phenotype, measuring the presence, amount and natural variance in phenolics composition of these fruits
A versatile targeted metabolomics method for rapid quantification of multiple classes of phenolics in fruit and beverages
Compelling evidence of the health benefits of phenolic compounds and their impact on food quality have stimulated the development of analytical methods for the identification and quantification of these compounds in different matrices in recent years. We have developed a targeted metabolomics method for the quantification of 140 phenolics,such as benzoates, phenylpropanoids, coumarins, stilbenes, dihydrochalcones and flavonoids in
fruit and tea extracts and wine using UPLC-MS/MS. Chromatography was optimised to achieve separation of the compounds over a period of 15 minutes and MRM transitions were selected for accurate quantification.
The effectiveness of the method was validated by studying the detection and quantification limits, the linearity ranges of instrumental response and the repeatability of the analysis. The method was validated for the analysis of apples, berries, green tea and red wine and represents a valuable tool for food quality evaluation and breeding studies