Development of Histamine in Fresh and Canned Tuna Steaks Stored under Different Experimental Temperature Conditions

Among biogenic amines, histamine is most frequently involved in foodborne intoxication. To evaluate histamine formation in tuna, several storage conditions were reproduced. An LC-MS/MS method was used for analytical determinations. Fresh tuna samples (not contaminated and grafted with tuna muscle naturally incurred with histamine at 6000 mg/kg) were stored at 4, 12, and 20 degrees C, and daily samples were collected for 6 days. The development of histamine was observed only in grafted tuna samples. At 4 degrees C, histamine formation progressed from 12.8 mg/kg (day 1) up to 68.2 mg/kg (day 6). At 12 degrees C, higher concentrations developed (23.9 mg/kg on day 1 up to 2721.3 mg/kg on day 6) relative to 20 degrees C (from 12.0 to 1681.0 mg/kg). It was found that at 4 degrees C, if grafted tuna was submerged in oil, histamine formation progressed more slowly. In a naturally contaminated sample, it was observed that the histamine distribution was uniform, while the normal cooking process did not affect the histamine level. Furthermore, it was found that the use of histamine-contaminated equipment for food handling may result in histamine formation in food. These results confirm the importance of implementing good hygiene practices and respecting the cold chain

Occurrence of ochratoxin a in different types of cheese offered for sale in Italy

The detection of Ochratoxin A (OTA) in the milk of ruminants occurs infrequently and at low levels, but its occurrence may be higher in dairy products such as cheese. The aim of this study was to investigate the presence of OTA in cheeses purchased in the metropolitan city of Bologna (Italy) and the surrounding area. For the analysis, a LC-MS/MS method with a limit of quantification (LOQ) of 1 µg/kg was used. OTA was detected in seven out of 51 samples of grated hard cheese (concentration range 1.3–22.4 µg/kg), while it was not found in the 33 cheeses of other types which were also analysed. These data show a low risk of OTA contamination for almost all types of cheese analysed. To improve the safety of cheese marketed in grated form, more regulations on cheese rind, which is the part most susceptible to OTA-producing moulds, should be implemented or, alternatively, producers should consider not using the rind as row material for grated cheese. It would be interesting to continue these investigations particularly on grated hard cheeses to have more data to update the risk assessment of OTA in cheese, as also suggested by EFSA in its 2020 scientific opinion on OTA

Occurrence of Histamine in Commercial Cat Foods under Different Storage Conditions

In fish-based foods, one of the effects of inappropriate storage can be the formation of biogenic amines. Among these, histamine is considered one of the most toxic. The purpose of the present study is to assess the occurrence of histamine in fish-based pet foods, and to evaluate the changes in histamine content during storage at different temperatures. For the analysis, an LC-MS/MS method was used. Fifty-eight pet foods were purchased, and an aliquot of them was analyzed just after opening the packages. Histamine was detected in 12 samples and concentrations ranged from 1.5 to 30.1 mg/kg. The remaining of each positive sample was divided into seven subsamples. One of them was used as a control sample and kept at -20 degrees C, while the other six were exposed to different environmental conditions. Samples exposed to room temperature showed no significant changes in histamine levels, while samples exposed to high temperatures showed significant increases in histamine content. Finally, samples exposed to refrigerator temperature showed a slight decrease in histamine levels. Under the experimental conditions, the EU limit of 100 mg/kg established for fishery products was never exceeded. These results seem to indicate a low risk of histamine intoxication in cats fed fish-based pet food

Content of Toxic Elements (Arsenic, Cadmium, Mercury, Lead) in Eggs from an Ethically Managed Laying Hen Farm

Domestic chicken farming has been promoted and spread in several Italian municipalities and worldwide as an aid to the self-consumption of domestically produced food. This study investigated the levels of four toxic elements (As, Cd, Hg, and Pb) in eggs from an ethical laying hen farm, comparing the element concentrations with those possibly present in supermarket eggs. A total of 201 eggs, 141 from the farm and produced by different hen genotypes, and 60 from the supermarket, were collected. The levels of the toxic elements were evaluated in the yolk, albumen, and eggshells of all eggs. The results show that the supermarket eggs’ yolk and albumen were more contaminated with lead, compared to the rural eggs. Contrarily, the mean content of arsenic was higher in the albumen and eggshells of the rural eggs, compared to the supermarket eggs. The cadmium content was below the LOQ (0.005 mg/kg) in all samples. The mercury content was below or around the LOQ in all rural eggs. Overall, the supermarket egg albumens were significantly more contaminated than the rural ones. No significant differences were found in quality parameters for both types of eggs. The toxic element values that were detected were in line with other studies in the literature. However, despite the concentrations found not representing a risk to the consumers’ health, the results of this study raise a potential food safety issue, and it would be desirable to set specific MRLs for eggs for consumers’ protection

Characterization and possible astrophysics applications of UV sensitive SiPM devices

The National Institute of Nuclear Physics (INFN) is involved in the R&D of Silicon Photomultiplier (SiPM) sensors optimized to detect near-UV (NUV) photon radiation in low-intensity photons and high-precision time mesaurements, in collaboration with the Bruno Kessler Foundation (FBK). The performances of 6×6 mm2 NUV-HD SiPMs with 30×30 μm2 microcell area and the possible prospects for production and packaging of multi-sensor modules for astrophysical applications are discussed in this paper

Upgrading the prototype Schwarzschild-Couder telescope camera to a wide-field, high-resolution instrument

The Schwarzschild-Couder Telescope (SCT) is a candidate technology for a medium-sized telescope within the Cherenkov Telescope Array. It is expected to yield substantial improvements in field of view and image resolution compared to traditional telescopes based on Davies-Cotton optics. To match the improved optical resolution, challenging requirements of high channel count and density at low power consumption have to be met by the camera. An initial prototype camera, with 1600 pixels spanning a 2.7? field of view, was installed on the prototype SCT in 2018. A project is now underway to upgrade the camera by increasing its pixel count and field of view by factors of 7 and 3, respectively (to 11,328 pixels and 8.0?). At the same time, the electronics design is being improved in order to lower the gamma-ray energy threshold and thereby provide an instrument especially well-suited for scientific studies related to extended sources and multimessenger astronomy