Fungal pretreatment of miscanthus for fermentable sugar production: experimental and techno-economic evaluation

Lignocellulosic biomass is an abundant, renewable feedstock for biorefineries, but pretreatment is usually required to overcome its high recalcitrance to biodegradation. Fungal pretreatment, an alternative process to traditional pretreatments, can enhance the enzymatic digestibility of the lignocellulosic biomass. Fungal pretreatment can be performed at low temperature, without added chemicals, and no wastewater generation. However, in comparison with traditional pretreatments, longer residence times, lower yields, and feedstock sterilization requirements make it challenging to implement. This work investigated the fungal pretreatment of the dedicated energy crop Miscanthus × giganteus with the white rot fungus Ceriporiopsis subvermispora. Fungal pretreatment of non-sterile miscanthus was performed in batch using miscanthus previously colonized with the fungus as inoculum. The process enhanced the enzymatic digestibility of miscanthus by 2-fold over that of untreated miscanthus, and was comparable to the pretreatment of sterilized miscanthus inoculated with a pure culture of the white rot fungus. The finished material from the unsterilized pretreatment was used as inoculum for two more generations in a sequential fungal pretreatment process, where no increase in enzymatic digestibility was observed. A propagation of indigenous fungi that out-colonized C. subvermispora was observed through the generations, showing that sterilization is a required step for the stability and reproducibility of fungal pretreatment. A techno-economic analysis of the production of fermentable sugars from miscanthus using fungal pretreatment showed that the process was not feasible at full biorefinery scale due to the high capital cost caused by long residence time, low feedstock bulk density, and low sugar yields

A Survey of Microbial Communities on Dry-Aged Beef in Commercial Meat Processing Facilities

Many artisanal meat professionals believe that the microbial populations on the outer crust of dry-aged beef contribute to variation in sensory profiles; however, to date there is minimal information about the microbes themselves that grow on commercially produced dry-aged beef. The microbiome of dry-aged beef bone-in strip loins (Institutional Meat Purchase Specifications #175) from 5 commercial dry aging facilities, including one utilizing ultraviolet light treatment, were surveyed to assess the microbial populations residing on and within each subprimal. Each strip loin was sampled at multiple spatial locations and depths, and the microbial sequences present in the samples were identified using a nextgeneration sequencing approach. Insufficient microbial DNA was isolated from ultraviolet-light-treated strip loins, indicating that this treatment eliminates all or most microbial growth on the meat. Sequencing results indicated that each establishment was producing meat with different microbial communities, based on Permutational Multivariate Analysis of Variance (P < 0.01) and clustering in the Principal Coordinates Analysis plot of Jaccard distances. The position on strip loins from which samples were taken had negligible influence on microbial community structure. Aging facility, and the relative unique environmental conditions within, was determined to be the only observed driver of community structure. Notable operational taxonomic units (OTUs) detected included the spoilage-associated bacterium Pseudomonas fragi and the fungal species Debaryomyces udenii and Penicillium polonicum. An OTU identified as Mucor sp. PG272 was found to be present in over 75% of all samples. This OTU may represent a species similar to Thamnidium, a mold that has been associated with product quality. This study established a general core microbiome for dry-aged beef observed in commercial facilities, variations of which may—as future research could indicate—contribute to distinct sensory properties

CANDOR & High Reliability: Response to Patient Harm - Arkansas Children\u27s CANDOR Journey

Harm in medicine is prevalent but preventable through adherence to high reliability principles and behaviors, and error prevention techniques. Harm reveals a complex interplay between the individuals and processes in our clinical systems and our patients and families. CANDOR, or communication and optimal resolution, grew out of 20 years of practice and research in human factors analysis, immediate and empathic communication with the affected patients and family members, and care and comfort to the caregivers. In this presentation, the implementation of the CANDOR journey at Arkansas Children\u27s will be discussed, showing how we integrated this approach with our pre-existing high reliability behaviors and tools. Presentation: 54:4

Mexican-American Caregivers\u27 Perceptions About Asthma Management: A Photovoice Study

Objective: The purpose of this study is to report how photovoice was used to (1) ascertain Mexican-American caregiver perspectives about asthma management, and (2) engage caregivers in dissemination. Methods: Eleven Mexican-American caregivers of children with asthma were recruited and given cameras to photograph what helped or hindered their ability to care for their child. Participants prioritized which images to share, discussed the images with the group, and wrote accompanying titles and captions in four photovoice sessions. Sessions were in Spanish and occurred in a community setting. Participants presented their work to peers and community members. Identified issues were subsequently categorized by investigators according to the four components of asthma care. Results: Participants prioritized 32 phototexts, the majority of which (n = 20, 63%) reflected activities associated with environmental control. Caregivers highlighted asthma triggers, and suggested ways to maintain indoor air quality (IAQ) through home cleaning. The need for policies that enforce smoking bans in shared housing and public places was identified as an important strategy to improve outdoor air quality. \u27Education for a Partnership in Asthma Care\u27 was represented in six (19%) phototexts. Five phototexts (16%) represented \u27Assessment and Monitoring\u27. Only one phototext (3.13%) primarily represented \u27Medications\u27. Conclusions: Results support the need for active partnerships between caregivers and providers. Photographs can serve as the basis for reciprocal education between patient and provider, especially in the area of environmental triggers. Provider visits should include assessment, strategies and resources to maximize IAQ. Photovoice facilitates caregivers\u27 ability to advocate for improved asthma management and health equity