12 research outputs found
Criteria of “persistent vomiting” in the WHO 2009 warning signs for dengue case classification
Introduction: Dengue is a viral disease that spreads rapidly in the tropic and subtropic regions of the world and causes 22,000 deaths annually. In 2009, the World Health Organization (WHO) released a new classification of dengue infections, which divided them into three categories: dengue without warning sign (D), dengue with warning sign (DWS), and severe dengue (SD). However, researchers have been using different criteria to define persistent vomiting; therefore, we aimed to evaluate the ability of the number of vomiting times in early prediction of SD development among D/DWS patients. Method: A hospital-based cohort study was conducted in Ben Tre-south of Vietnam. We enrolled confirmed dengue patients with D and DWS at admission. The final classification was determined on the discharged day for every patient based on the classification of WHO 2009 without using vomiting symptom, using the receiver operating characteristic (ROC) curve to evaluate the ability of the number of vomiting times in early prediction of SD development among D/DWS patients. Result: The prevalence of vomiting symptom was higher in SD group than D/DWS group (92 versus 46 %, p = 0.006), and the median of the number of vomiting times was higher in SD group than D/DWS group (2.5 versus 0, p = 0.001). To distinguish SD from D/DWS, the ROC curve of the number of vomiting episodes showed that the area under the curve was 0.77; with the cut point of two, the sensitivity and specificity were 92 and 52 %, respectively. Conclusion: The number of vomiting times could be a good clinical sign which can early predict SD from the group of D/DWS. We suggest the definition of persistent vomiting should be vomiting two times or more per day
A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children
Receiver operating characteristic (ROC) curves comparing antithrombin III and angiotensinogen markers for SD-SPL prediction.
<p>Area under the curve (AUC) for antithrombin III = 0.85, AUC for angiotensinogen = 0.83 and AUC for combination of two markers = 0.87.</p
Western-blot analysis validating iTRAQs results for angiotensinogen and antithrombin III.
<p>Representative protein bands in DWS (n = 10) and SD-SPL (n = 6) for AGT (A) and for AT III (C). Quantification of relative protein expression of AGT (B) and AT III (D), based on normalized densitometry to healthy control, *p < 0.05.</p
List of differentially expressed plasma proteins of SD-SPL compared with those of DWS (p<0.05).
<p>List of differentially expressed plasma proteins of SD-SPL compared with those of DWS (p<0.05).</p
Biological analysis of identified proteins using PANTHER classification.
<p>Biological analysis of identified proteins using PANTHER classification.</p
Identification of angiotensinogen (AGT) and antithrombin III (AT III) proteins.
<p>Representative precursor ion and MS/MS spectra of reporter ions from peptide ALQDQLVLVAAK and DPTFIPAPIQAK of AGT (A) and from peptide RVWELSK and ATEDEGSEQKIPEATNR of AT III (B). Quantification was derived from the signal intensities of eight iTRAQ reporter ions.</p
Multivariate logistic regression analysis of SD-SPL predictive diagnosis.
<p>Multivariate logistic regression analysis of SD-SPL predictive diagnosis.</p
Study profile.
<p>Of 113 patients enrolled, 63 had laboratory-confirmed dengue infection. One patient hospitalized in shock presentation was excluded. Six SD-SPL and ten DWS, with early stage plasma collected, were included in the current study.</p