Efficacy of the motile sperm organelle morphology examination (MSOME) in predicting pregnancy after intrauterine insemination

Background: Although the motile sperm organelle morphology examination (MSOME) was developed merely as a selection criterion, its application as a method for classifying sperm morphology may represent an improvement in the evaluation of semen quality. The aim of this study was to determine the prognostic value of normal sperm morphology using MSOME with regard to clinical pregnancy (CP) after intrauterine insemination (IUI).Methods: A total of 156 IUI cycles that were performed in 111 couples were prospectively analysed. Each subject received 75 IU of recombinant FSH every second day from the third day of the cycle. Beginning on the 10th day of the cycle, follicular development was monitored by vaginal ultrasound. When one or two follicles measuring at least 17 mm were observed, recombinant hCG was administered, and IUI was performed 12-14 h and 36-40 h after hCG treatment. Prior to the IUI procedure, sperm samples were analysed by MSOME at 8400x magnification using an inverted microscope that was equipped with DIC/Nomarski differential interference contrast optics. A minimum of 200 motile spermatozoa per semen sample were evaluated, and the percentage of normal spermatozoa in each sample was determined.Results: Pregnancy occurred in 34 IUI cycles (CP rate per cycle: 21.8%, per patient: 30.6%). Based on the MSOME criteria, a significantly higher percentage of normal spermatozoa was found in the group of men in which the IUI cycles resulted in pregnancy (2.6+/-3.1%) compared to the group that did not achieve pregnancy (1.2+/-1.7%; P = 0.019). Logistic regression showed that the percentage of normal cells in the MSOME was a determining factor for the likelihood of clinical pregnancy (OR: 1.28; 95% CI: 1.08 to 1.51; P = 0.003). The ROC curve revealed an area under the curve of 0.63 and an optimum cut-off point of 2% of normal sperm morphology. At this cut-off threshold, using the percentage of normal sperm morphology by MSOME to predict pregnancy was 50% sensitive with a 40% positive predictive value and 79% specificity with an 85% negative predictive value. The efficacy of using the percentage of normal sperm morphology by MSOME in predicting pregnancy was 65%.Conclusions: The present findings support the use of high-magnification microscopy both for selecting spermatozoa and as a routine method for analysing semen before performing IUI

Architecture of a memory manager for an MPEG-2 video decoding circuit

A study of the MPEG-2 video decoding standard in Main Profile @ Main Level has been performed, comparing the different solutions existing for the VLSI implementation of the basic functions (Huffman decoding, IDCT...) included in the standard. Afterwards, a new dynamically configurable architecture is proposed for the memory manager, which is necessary to deal with the large data flow inside the decoder. It is aimed at interfacing the external memory, arbitrating the access requests coming from the different decoding units and allowing generic memory requests through the definition of virtual addresses. It is shown that, by means of a particular data organization, the circuit requires an external memory, which is a 2-MB DRAM in fast page or EDO mode, accessible via a 64-bit bus. The memory manager works at 27 MHz and allows a real-time decoding for MP @ ML bitstreams. It has been synthesized in a 0.8-mu m two-metal CMOS technology and presents a total area of 5.4 mm(2) for 6500 gates

Combined line-based architecture for the 5-3 and 9-7 wavelet transform of JPEG2000

The wavelet transform is a very promising tool for image compression. In JPEG2000, two filter banks are used, one an integer lossless 5-3 filter, and one a lossy 9-7. A combined architecture for the 5-3 and 9-7 transforms with minimum area is presented in this paper. The lifting scheme is used to realize a fast wavelet transform. Two lines are processed at a time. This line-based architecture allows minimum memory requirement and fast calculation. The pipeline and the optimization of the operations provide speed, while the combination of the two transforms in one structure contributes to saving the area. On a VIRTEXE1000-8, de coding of 2 pixels per clock cycle can be performed at 110 MHz. Only 19% of the total area of the VIRTEXE1000 is needed. Compared to existing architectures, memory resource and area can be reduced thanks to the solution proposed in this paper