Fractionation of human immune γ-globulin

Equine and bovine serum proteins have recently been fractionated by means of a physical method utilizing an electrophoretic adaptation of the principles of the Clusius column (l-4), first described and tested by Kirkwood (5) and Nielsen (6). The method of electrophoresis-convection has now been applied to the fractionation of human γ-globulin. The γ-globulin was prepared by ethanol fractionation (7) from the plasma of individuals hyperimmunized to Hemophilus pertussis organisms. The resulting fractions of γ-globulin have been characterized electrophoretically, and the protective antibody activity and agglutinin titer have been measured

An association of benthic foraminifera and gypsum in Holocene sediments of estuarine Chesapeake Bay, USA

Two cores of Holocene sediments recovered from the Cape Charles Channel of Chesapeake Bay yielded radiocarbon ages of about 6.8 to 5.8 ka for the lower intervals. Fossil foraminifera preserved in these lower sediments are dominated by species of Elphidium, which make up about 90% of the assemblage throughout, and probably signify deposition in hypersaline waters. Buccella frigida and Ammonia beccarii are the only other species commonly present. Hypersalinity of bottom waters seems to have been maintained by water-density stratification in a basin-like section of the channel. In core PTXT -4-P-I transition to modem Chesapeake conditions, in which numbers of Ammonia beccarii exceed those of Elphidium, commenced about 400 years ago. In core PTXT -3-P-2 hypersalinity is further signified by the presence of abundant euhedral crystals of gypsum in association with the fossil Elphidium. This occurrence of gypsum is not attributed to palaeoclimatic aridity, but rather to inflow of groundwater from adjacent gypsiferous Miocene strata. The study shows that in palaeoclimatic investigations the significance of the presence of gypsum should be evaluated with caution - it does not necessarily signify an evaporative regime

Trans-Atlantic Death Methods:Disciplinarity shared and challenged by a common language

The different countries that death and dying researchers reside within often shape not only research agendas but also research methodologies. The United Kingdom and the United States are two examples of countries that share a common language and intellectual history but their discourses on death have been very different. These differences are partly explained through cultural practices, and also government funding of research, definitions of death and end-of-life planning education. In this article, we argue that early death scholarship in the United States impacted death research and outcomes in both the US and the UK, but that recent scholarship in both countries has caused the two countries to diverge in two major areas: (1) the methodological approaches to death studies and (2) the educational training of medical and hospice personnel in direct contact with the dying. We argue that in order for death studies to fully benefit from trans-Atlantic dialogue on death, both countries need to move towards a more integrated trans-disciplinary model

Dual-Process Theory of Racial Isolation, Legal Cynicism, and Reported Crime

Why is neighborhood racial composition linked so strongly to police-reported crime? Common explanations include over-policing and negative interactions with police, but police reports of crime are heavily dependent on resident 911 calls. Using Sampson’s concept of legal cynicism and Vaisey’s dual-process theory, we theorize that racial concentration and isolation consciously and nonconsciously influence neighborhood variation in 911 calls for protection and prevention. The data we analyze are consistent with this thesis. Independent of police reports of crime, we find that neighborhood racial segregation in 1990 and the legal cynicism about crime prevention and protection it engenders have lasting effects on 911 calls more than a decade later, in 2006–2008. Our theory explains this persistent predictive influence through continuity and change in intervening factors. A source of cumulative continuity, the intensification of neighborhood racial concentration and isolation between 1990 and 2000, predicts 911 calls. Likewise, sources of change—heightened neighborhood incarceration and home foreclosures during the financial crisis in 2006–2008—also predict these calls. Our findings are consistent with legal cynicism theory’s focus on neighborhood disadvantage, racial isolation, and concerns about police protection and crime prevention; they correspond less with the emphasis of procedural justice theory on police legitimacy

Sedimentation rates test models of oceanic detachment faulting

This is the accepted manuscript version.The final version is available from Wiley at http://onlinelibrary.wiley.com/doi/10.1002/2014GL061555/full.Long-lived detachment faults play an important role in the construction of new oceanic crust at slow-spreading mid-oceanic ridges. Although the corrugated surfaces of exposed low-angle faults demonstrate past slip, it is difficult to determine whether a given fault is currently active. If inactive, it is unclear when slip ceased. This judgment is crucial for tectonic reconstructions where detachment faults are present, and for models of plate spreading. We quantify variation in sediment thickness over two corrugated surfaces near 16.5°N at the Mid-Atlantic Ridge using near-bottom CHIRP data. We show that the distribution of sediment and tectonic features at one detachment fault is consistent with slip occurring today. In contrast, another corrugated surface 20 km to the south shows a sediment distribution suggesting that slip ceased ~150,000 years ago. Data presented here provide new evidence for active detachment faulting, and suggest along-axis variations in fault activity occur over tens of kilometers.This work was supported by the National Science Foundation grant number OCE-1155650

Cauli: a mouse strain with an Ift140 mutation that results in a skeletal ciliopathy modelling jeune syndrome

Cilia are architecturally complex organelles that protrude from the cell membrane and have signalling, sensory and motility functions that are central to normal tissue development and homeostasis. There are two broad categories of cilia; motile and non-motile, or primary, cilia. The central role of primary cilia in health and disease has become prominent in the past decade with the recognition of a number of human syndromes that result from defects in the formation or function of primary cilia. This rapidly growing class of conditions, now known as ciliopathies, impact the development of a diverse range of tissues including the neural axis, craniofacial structures, skeleton, kidneys, eyes and lungs. The broad impact of cilia dysfunction on development reflects the pivotal position of the primary cilia within a signalling nexus involving a growing number of growth factor systems including Hedgehog, Pdgf, Fgf, Hippo, Notch and both canonical Wnt and planar cell polarity. We have identified a novel ENU mutant allele of Ift140, which causes a mid-gestation embryonic lethal phenotype in homozygous mutant mice. Mutant embryos exhibit a range of phenotypes including exencephaly and spina bifida, craniofacial dysmorphism, digit anomalies, cardiac anomalies and somite patterning defects. A number of these phenotypes can be attributed to alterations in Hedgehog signalling, although additional signalling systems are also likely to be involved. We also report the identification of a homozygous recessive mutation in IFT140 in a Jeune syndrome patient. This ENU-induced Jeune syndrome model will be useful in delineating the origins of dysmorphology in human ciliopathies

Procedural Signaling, Party Loyalty, and Traceability in the U.S. House of Representatives

In this article, we take advantage of a new source of data providing updates from the Majority Leader’s Office that signal the leadership’s positions on floor votes. We offer a more nuanced explanation of voting in the U.S. House as our findings suggest that not all procedural votes are created equal. While the most liberal members of the party vote with the leadership on procedural votes at high rates and nearly 100 percent of the time when signaled by the majority leader, moderate members are significantly less likely to support the party and are not responsive to these signals.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline

Simian hemorrhagic fever virus infection of rhesus macaques as a model of viral hemorrhagic fever: Clinical characterization and risk factors for severe disease

AbstractSimian Hemorrhagic Fever Virus (SHFV) has caused sporadic outbreaks of hemorrhagic fevers in macaques at primate research facilities. SHFV is a BSL-2 pathogen that has not been linked to human disease; as such, investigation of SHFV pathogenesis in non-human primates (NHPs) could serve as a model for hemorrhagic fever viruses such as Ebola, Marburg, and Lassa viruses. Here we describe the pathogenesis of SHFV in rhesus macaques inoculated with doses ranging from 50PFU to 500,000PFU. Disease severity was independent of dose with an overall mortality rate of 64% with signs of hemorrhagic fever and multiple organ system involvement. Analyses comparing survivors and non-survivors were performed to identify factors associated with survival revealing differences in the kinetics of viremia, immunosuppression, and regulation of hemostasis. Notable similarities between the pathogenesis of SHFV in NHPs and hemorrhagic fever viruses in humans suggest that SHFV may serve as a suitable model of BSL-4 pathogens