Response of lymphocyte subsets and cytokines to Shenyang prescription in Sprague-Dawley rats with tongue squamous cell carcinomas induced by 4NQO

BACKGROUND: The study was designed to investigate immunocompetence in relation to cancer progression in rat and to assess the effect of the traditional Chinese anti-cancer medicine, "Shenyang" prescription, on immunity. METHODS: 4-Nitroquinoline-1-oxide (4NQO) was administered to 80 Sprague-Dawley (SD) rats via the drinking water for up to 36 weeks. Tongue squamous cell carcinoma (SCC) was confirmed by pathological examination in 61 rats. "Shenyang" prescription was administered to subgroups of these rats, and blood samples were taken before and after treatment. Lymphocyte subsets were determined by flow cytometry. Serum Th1 and Th2-type cytokines were assessed by an enzyme-linked immunosorbent assay. RESULTS: As the cancer progressed at the tongue root, the percentage of CD3+CD4+ T lymphocytes and NK cells and the levels of IFN-γ and IL-2 decreased gradually, while the percentage of CD3+CD8+ T lymphocytes and the levels of IL-4 and IL-10 increased. The CD4+/CD8+ ratios were lower in the cancer groups than in the control group. However, after administering "Shenyang" prescription, the levels of CD3+CD4+ T lymphocytes, NK cells, IFN-γ and IL-2 increased, while the CD3+CD8+ T lymphocyte counts and the levels of IL-4 and IL-10 decreased. CONCLUSION: 4NQO-induced lesions were good models for exploring oral cavity carcinogenesis. The rats with 4NQO-induced SCC demonstrated abnormalities in lymphocyte subsets and a shift from Th1-type to Th2-type, which were good models for assessing the effect of anticancer agent on immunity. Oral cancer progression was associated with an aggressive disturbance of immune function. "Shenyang" prescription has the ability to improve the disturbance of immune function

Understanding Chinese Domestic Travelers’ Trip Patterns to Mount Fanjing

The purpose of this study is to identify Chinese domestic travelers’ trip patterns to Mount Fanjing. Data analysis is based on 200 questionnaires completed by travelers to the destination. Consistent with the LCF model, the researchers recognize five trip patterns: the single destination pattern, en-route pattern, base-camp pattern, regional tour pattern, and trip-chaining pattern. In addition, the researchers examine factors that influence Chinese travelers’ decisions on single-destination and multi-destination trips. Time constraints and destination familiarity have significant impacts on Chinese travelers’ destination choice. The research findings have implications for researchers to understand Chinese domestic travelers’ behavior and for local governments to develop their destination marketing strategies

Correction Method for Logging Curves in Clay-Rich Tight Glutenite Reservoir: Upper Wuerhe Formation in Mahu Oilfield, China

Mahu Oilfield is the largest tight glutenite oilfield in the world, and the upper Wuerhe formation is an important succeeding exploration horizon. However, the upper Wuerhe formation in the Mahu 1 zone has a high clay content, which can lead to serious wellbore collapse. Meanwhile, the horizontal well logging is not corrected. These factors lead to the inconsistency between the logging interpretation results and the oil test results. The interpretation precision of the clay content, water saturation, and porosity, which are crucial to reservoir evaluation, is very low. In this paper, a workflow of logging curve correction using multiple methods is proposed. The multiple linear fitting is used to correct boreholes, and then histogram frequency distribution matching is used to standardize multi-well logging curves. Finally, the optimization method is used to build a volume model based on skeleton analysis, and the results are calibrated with core analysis results. Horizontal well density logs are corrected using adjacent vertical well logs. The interpretation results of clay content, water saturation, and porosity with high precision are obtained. The reservoir interpretation is more in line with the oil test results than the original interpretation. The clay content distribution is more reasonable

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field