Assessment of selected Saudi and Yemeni plants for mosquitocidal activities against the yellow fever mosquito Aedes aegypti

© 2019 by the authors. Marine organisms are recognized as a source of compounds with interesting biological activities. Vibrio neocaledonicus has been reported on for its high effectiveness against corrosion in metals but it has been little studied for its chemical and biological activities. In this study, four compounds were isolated from V. neocaledonicus: indole (1); 1H-indole-3-carboxaldehyde (2); 4-hydroxybenzaldehyde (3) and Cyclo (-Pro-Tyr) (4); using a bioassay-guided method, since in a previous study it was found that the ethyl acetate extract was active on the enzymes acetylcholinesterase (AChE), alpha-glucosidase (AG) and xanthine oxidase (XO). The inhibitory activities of the three compounds against AChE, AG and XO was also evaluated. In addition, the enzymatic inhibitory activity of indole to the toxins from the venom of Bothrops asper was tested. Results showed that indole exhibited strong inhibitory activity to AG (IC50 = 18.65 ± 1.1 µM), to AChE, and XO (51.3% and 44.3% at 50 µg/mL, respectively). 1H-indole-3-carboxaldehyde displayed strong activity to XO (IC50 = 13.36 ± 0.39 µM). 4-hydroxybenzaldehyde showed moderate activity to XO (50.75% at 50 µg/mL) and weak activity to AChE (25.7% at 50 µg/mL). Furthermore, indole showed a significant in vitro inhibition to the coagulant effect induced by 1.0 µg of venom. The findings were supported by molecular docking. This is the first comprehensive report on the chemistry of V. neocaledonicus and the bioactivity of its metabolites

In vitro antioxidant properties and phenolic composition of Salvia virgata Jacq. from Turkey

Antioxidant activities and phenolic compositions of the active fractions of Salvia virgata Jacq. (Lamiaceae) from Turkey were examined. The aerial part of S. virgata was extracted with different solvents in an order of increasing polarity such as hexane, ethyl acetate, methanol, and 50% aqueous methanol using a Soxhlet apparatus. Water extract was also prepared from S. virgata by reflux. All solvent fractions were investigated for their total phenolic contents, total flavonoids, flavonols, qualitative-quantitative compositions (by HPLC-PDA analysis), iron(III) reductive activities, free radical scavenging activities (using DPPH center dot), and effect upon linoleic acid peroxidation activities; also, the peroxidation level was determined by the TBA method. The results of activity tests given as IC50 values were estimated from nonlinear algorithm and compared with standards, viz., butylated hydroxytoluene, ascorbic acid, and gallic acid. Polar fractions were found to be more active for free radical activity whereas nonpolar fractions protected the peroxidation of linoleic acid. Rosmarinic acid was the most abundant component in the extracts, followed by caffeic acid and lutelin-7-O-glycoside

In vitro antioxidant properties and phenolic composition of Salvia halophila Hedge from Turkey

An endemic plant of Turkey Salvia halophila Hedge (Lamiaceae) was examined for its antioxidant activity and phenolic compositions. The aerial part of S. halophila was extracted with different solvents in an order of increasing polarity such as hexane, ethyl acetate, methanol, and 50% methanol using a Soxhlet apparatus. Water extract was also prepared from S. halophila by reflux. All solvent fractions were investigated for their total phenolic contents, flavonoids, flavonols, qualitative-quantitative compositions, iron(III) reductive activities, free radical scavenging activities and the effect upon linoleic acid peroxidation activities. The peroxidation level was also determined by the TBA method. The results of activity tests given as IC50 values were estimated from non-linear algorithm and compared with standards via BHT, ascorbic acid, gallic acid. Polar fractions were found more active among the others in free radical activity system whereas non-polar fractions protected the peroxidation of linoleic acid. Rosmarinic acid was the most abundant component, in the extracts. (C) 2011 Elsevier Ltd. All rights reserved

Antioxidant, anti-inflammatory, anti-nociceptive activities and composition of Lythrum salicaria L. extracts

Lythrum salicaria (purple loosestrife) known as "Tibbi hevhulma" in Turkish is used for its several beneficial health effects against as diarrhea, chronic intestinal catarrh, hemorrhoid and eczema in the form of a decoction or a fluid extract and to treat varicose veins, bleeding of the gums, hemorrhoid and eczema, externally. Dried herbal parts of Lythrum salicaria L. (Lythraceae) were sequentially extracted with different solvents such as petroleum ether, ethyl acetate, methanol and 50% aqueous methanol. Water extract of Lythrum salicaria was also prepared under reflux. Antioxidant, anti-inflammatory and anti-nociceptive activities of all the extracts were investigated using in vitro and in vivo methods, respectively. Free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH center dot assay), iron(III) reductive activity, capacity of the inhibition of linoleic acid peroxidation and MDA formation, anti-nociceptive activity (p-benzoquinone-induced abdominal constriction test) and anti-inflammatory activity (carrageenan-induced hind paw edema model) were used for all the extracts. In addition, the content of total phenolics, flavonoids and flavonols in all the extracts were determined with spectrophotometric methods. Results were compared with reference antioxidants via ascorbic acid, butylated hydroxytoluene, and gallic acid. Qualitative and quantitative compositions of all the extracts were analysed using a HPLC-PDA system. Polar fractions were found to be rich in flavonoids such as isovitexin and isoorientin. (c) 2006 Elsevier Ireland Ltd. All rights reserved

Iron(III) reducing and antiradical activities of three Sideritis from Turkey

Objective: The antiradical activities of the various solvent extracts of Sideritis species are investigated here for the first time

CHEMICAL COMPOSITION AND ANTIBACTERIAL EVALUATION OF THE ESSENTIAL OILS OF FERULAGO AUCHERI BOISS. FROM DIFFERENT LOCALITIES

Air dried and crushed fruits of Ferulago aucheri Boiss. (Apiaceae) from Southern and Western regions of Turkey were subjected to hydrodistillation to obtain essential oils, which were subsequently analyzed by GC-FID and GC/MS. Seventy seven compounds representing 95.9% and 127 compounds representing 86.5% of the fruit oils have been characterized for samples of F. aucheri from Antalya (oil A) and Manisa (oil B) provinces, respectively. Monoterpenes comprised 83.6% of the oil A with limonene (43.1%), alpha-pinene (18.3%) and myrcene (7.0%) as major constituents. Sesquiterpenes (72.7%) prevailed in the oil B, with germacrene D (25.7%) and (2E,6E)-farnesol (8.0%) as major constituents. Both essential oils were also evaluated for their antibacterial activity against eight Gram (+) and Gram (-) strains by microdilution method. The oils displayed moderate to weak inhibitory activity (MIC 0.25-3 mg/mL). The oils were found to be the most active against Pseudomonas aeruginosa when compared with antimicrobial standards. Antibacterial activity of the oils appeared non-selective when comparing Gram (+) and Gram (-) strains