Polarization-dependence of anomalous scattering in brominated DNA and RNA molecules, and importance of crystal orientation in single- and multiple-wavelength anomalous diffraction phasing

In this paper the anisotropy of anomalous scattering at the Br K-absorption edge in brominated nucleotides is investigated, and it is shown that this effect can give rise to a marked directional dependence of the anomalous signal strength in X-ray diffraction data. This implies that choosing the correct orientation for crystals of such molecules can be a crucial determinant of success or failure when using single- and multiple-wavelength anomalous diffraction (SAD or MAD) methods to solve their structure. In particular, polarized absorption spectra on an oriented crystal of a brominated DNA molecule were measured, and were used to determine the orientation that yields a maximum anomalous signal in the diffraction data. Out of several SAD data sets, only those collected at or near that optimal orientation allowed interpretable electron density maps to be obtained. The findings of this study have implications for instrumental choices in experimental stations at synchrotron beamlines, as well as for the development of data collection strategy programs

Soutiens perçus, engagements affectifs et succès de carrière subjectif : une approche en termes de profils

Perceived support, affective commitments and subjective career success: a person-centred approach (Soutiens perçus, engagements affectifs et succès de carrière subjectif : une approche en termes de profils)

Differences in the ubiquitination of p53 by Mdm2 and the HPV protein E6

AbstractThe human papillomavirus (HPV) protein E6 can promote the ubiquitination of the p53 tumour suppressor in vitro, providing an explanation for the ability of E6 to induce p53 degradation in vivo and contribute to the potential tumorigenic effect of the virus. Instead, in non-infected cells, p53 levels are primarily destabilised by the ubiquitin E3 ligase activity of the Mdm2 protein. Here we have compared the effects of E6 and Mdm2 on p53 ubiquitination in vivo. We show that whereas in the presence of Mdm2 proteasome inhibitors induce the accumulation of ubiquitinated forms of p53, this does not occur in the presence of E6. Accordingly, we confirm that the effect of E6 and p53 is independent of the six C-terminal lysine residues in p53, which have previously been described to play an important role for effective ubiquitination and degradation of 53 mediated by Mdm2. We also show that other yet unidentified residues in p53 are also susceptible to ubiquitination. These results indicate that E6 does not induce ubiquitination of p53 in the same way as Mdm2 in order to promote its degradation, suggesting important differences between the Mdm2 and E6 effects on p53 degradation

[C II] 158 μm Emission as a Star Formation Tracer

The [C II] 157.74 μm transition is the dominant coolant of the neutral interstellar gas, and has great potential as a star formation rate (SFR) tracer. Using the Herschel KINGFISH sample of 46 nearby galaxies, we investigate the relation of [C II] surface brightness and luminosity with SFR. We conclude that [C II] can be used for measurements of SFR on both global and kiloparsec scales in normal star-forming galaxies in the absence of strong active galactic nuclei (AGNs). The uncertainty of the Σ_([C II]) – Σ_(SFR) calibration is ±0.21 dex. The main source of scatter in the correlation is associated with regions that exhibit warm IR colors, and we provide an adjustment based on IR color that reduces the scatter. We show that the color-adjusted Σ_([C II]) – Σ_(SFR) correlation is valid over almost five orders of magnitude in Σ_(SFR), holding for both normal star-forming galaxies and non-AGN luminous infrared galaxies. Using [C II] luminosity instead of surface brightness to estimate SFR suffers from worse systematics, frequently underpredicting SFR in luminous infrared galaxies even after IR color adjustment (although this depends on the SFR measure employed). We suspect that surface brightness relations are better behaved than the luminosity relations because the former are more closely related to the local far-UV field strength, most likely the main parameter controlling the efficiency of the conversion of far-UV radiation into gas heating. A simple model based on Starburst99 population-synthesis code to connect SFR to [C II] finds that heating efficiencies are 1%-3% in normal galaxies

End-of-pipe Waste Analysis and Integrated Solid Waste Management Plan

A ten-year integrated solid waste management plan was established for the University of the Philippines Los Baños which complies with the provisions of RA 9003. An end-of-pipe Waste Analysis and Characterization Study (WACS) was performed to identify the classification of wastes in UPLB. Waste generation was found to be 593.67 kg/day on the average and is expected to increase by 2% per year which is 709.49 kg/day on the year 2027. The waste composition by weight of the non-biodegradable wastes are as follows: plastic (55.68%); paper (35.77%); glass bottles (5.22%); metal (2.77%); and residuals (0.55%). A large portion of the wastes, which is 99.45% by weight, are recyclables.  The loose density of wastes is 131.93 kg/ m3. Feasible collection points were assigned to improve efficiency of the collection of wastes in the university. Building units inside the campus were clustered and was assigned to dispose wastes to a single temporary storage facility per cluster. There are 181 units of 240-L garbage bin needed for the 39 clusters in UPLB. Two sets of dimensions of a proposed temporary storage facility were provided for the temporary storage facility; 5 2 2.2 m and 3.5 2 2.2 m. Conceptual design and structural plans of the materials recovery facility were provided. Mass balance was performed, and the theoretical diversion efficiency of the materials recovery facility is 99.445%

Heating and cooling of the neutral ISM in the NGC4736 circumnuclear ring

The manner in which gas accretes and orbits within circumnuclear rings has direct implications for the star formation process. In particular, gas may be compressed and shocked at the inflow points, resulting in bursts of star formation at these locations. Afterwards the gas and young stars move together through the ring. In addition, star formation may occur throughout the ring, if and when the gas reaches sufficient density to collapse under gravity. These two scenarios for star formation in rings are often referred to as the `pearls on a string' and `popcorn' paradigms. In this paper, we use new Herschel PACS observations, obtained as part of the KINGFISH Open Time Key Program, along with archival Spitzer and ground-based observations from the SINGS Legacy project, to investigate the heating and cooling of the interstellar medium in the nearby star-forming ring galaxy, NGC4736. By comparing spatially resolved estimates of the stellar FUV flux available for heating, with the gas and dust cooling derived from the FIR continuum and line emission, we show that while star formation is indeed dominant at the inflow points in NGC 4736, additional star formation is needed to balance the gas heating and cooling throughout the ring. This additional component most likely arises from the general increase in gas density in the ring over its lifetime. Our data provide strong evidence, therefore, for a combination of the two paradigms for star formation in the ring in NGC4736.Comment: accepted for publication in A&