Aplicación de un abordaje lipidómico para el estudio de la bioactividad in vitro de oxilipinas vegetales.

La exposición a xenocompuestos tóxicos aumenta considerablemente los procesos inflamatorios, así como los niveles de estrés oxidativo. Dichos eventos son los ejes centrales en la progresión y gravedad de numerosas enfermedades. Las oxilipinas vegetales, representadas por los fitoprostanos y los fitofuranos (FitoPs y FitoFs, respectivamente), constituyen una amplia familia de metabolitos secundarios de origen vegetal, originados por la oxidación de ácidos grasos (ácido linolénico y linoleico). Los FitoP y FitoF presentan una gran similitud estructural con los isoprostanoides y prostaglandinas, los cuales suponen una importante herramienta molecular para el mantenimiento del equilibrio redox celular, para la modulación de los procesos inflamatorios y de agregación plaquetaria. La analogía estructural de estas oxilipinas vegetales con los isoprostanoides y prostaglandinas y varios ensayos nutricionales han llevado a la conclusión de que estos mediadores pueden prevenir fisiopatológicos importantes. Asimismo, las oxilipinas vegetales han sido identificadas en una gran variedad de alimentos de origen vegetal, mostrando respuestas proinflamatorias en algunos FitoPs. En este marco, hay que tener en cuenta que dichas sustancias una vez ingeridas y asimiladas por los mamíferos pueden desarrollar funciones al tener la capacidad de interactuar con diferentes dianas moleculares. En este contexto, uno de los propósitos de este trabajo fue analizar la capacidad que presentan las oxilipinas identificadas individualmente para prevenir los procesos de estrés oxidativo e inflamación y determinar las posibles interacciones moleculares que se producen en situaciones fisiopatológicas. En el marco de la presente Tesis Doctoral se investigó mediante un modelo in vitro de simulación inflamatoria (estimulados por lipopolisacáridos) la capacidad de los diferentes FitoPs y FitoFs, considerados individualmente,para prevenir dichos efectos, profundizando en las formas de acoplamiento molecular responsables de los efectos biológicos desencadenados. De igual forma, a través de la línea monocítica THP-1 se realizó un enfoque lipidómico que proporcionó datos sobre las funciones antioxidantes y antiinflamatorias de las oxilipinas vegetales. Asimismo, 9-D1t-FitoP estimuló a los receptores Eprostanoides de la PEG2, los cuales mejoraron la agregación plaquetaria inducida por ADP. Además, dicho FitoP se mostró como un posible receptor potencial al unirse a EP3. Los resultados obtenidos han esclarecido las propiedades antiiflamatorias y antioxidantes de FitoP y FitoF, sin embargo son necesarios más estudios in vitro e in vivo para arrojar luz sobre la capacidad funcional de las fracciones bioaccesibles y biodisponibles de estos compuestos.Enfermerí

Reliability and Validity of a Stress Scale in Public Employees from Murcia (Spain)

Stress is common in all work environments. Technostress and the difficulty of separating the family arena from the work environment are some of the new and emerging risks faced by companies, employees and society in general. Most of the available instruments for measuring stress in workers have been focused on education professionals and healthcare workers. Therefore, it is necessary to validate simple and friendly-use tools to detect stress levels in public workers. The aim of this study was to determine the internal consistency of an adapted version of the Student Stress Inventory-Stress Manifestations (SSI-SM) for public employees and to determine if high-stress levels are related to personal and work-related factors. A cross-sectional and descriptive study was conducted from October 2016 to February 2019 including 468 Spanish public workers based in Murcia. An adapted version of the SSI-SM was administered and data on personal and work-related factors were collected. Results showed that all of the factors had Cronbach’s α over 0.700, and no items need to be deleted due to correlations with the factor exceeding 0.300. Factor 1, “Self-concept”, has a Cronbach’s α of 0.868, with values of 15.62 ± 4.99; factor 2, “Sociability”, Cronbach’s α: 0.853, with mean values of 13.33 ± 4.17; factor 3, “Somatization”, Cronbach’s α: 0.704, mean value of 5.35 ± 1.90 and: factor 4, “Uncertainty”, Cronbach’s α: 0.746, with a mean value of 8.19 ± 2.51. In conclusion, the internal consistency of the adapted SSI-SM for public employees with different work positions and shifts has been validated and determined. This study provides a useful tool for the early detection of stress in public employees and may be potentially useful for preventing the harmful consequences of stres

Reliability and Validity of a Stress Scale in Public Employees from Murcia (Spain)

Stress is common in all work environments. Technostress and the difficulty of separating the family arena from the work environment are some of the new and emerging risks faced by companies, employees and society in general. Most of the available instruments for measuring stress in workers have been focused on education professionals and healthcare workers. Therefore, it is necessary to validate simple and friendly-use tools to detect stress levels in public workers. The aim of this study was to determine the internal consistency of an adapted version of the Student Stress Inventory-Stress Manifestations (SSI-SM) for public employees and to determine if high-stress levels are related to personal and work-related factors. A cross-sectional and descriptive study was conducted from October 2016 to February 2019 including 468 Spanish public workers based in Murcia. An adapted version of the SSI-SM was administered and data on personal and work-related factors were collected. Results showed that all of the factors had Cronbach’s α over 0.700, and no items need to be deleted due to correlations with the factor exceeding 0.300. Factor 1, “Self-concept”, has a Cronbach’s α of 0.868, with values of 15.62 ± 4.99; factor 2, “Sociability”, Cronbach’s α: 0.853, with mean values of 13.33 ± 4.17; factor 3, “Somatization”, Cronbach’s α: 0.704, mean value of 5.35 ± 1.90 and: factor 4, “Uncertainty”, Cronbach’s α: 0.746, with a mean value of 8.19 ± 2.51. In conclusion, the internal consistency of the adapted SSI-SM for public employees with different work positions and shifts has been validated and determined. This study provides a useful tool for the early detection of stress in public employees and may be potentially useful for preventing the harmful consequences of stress