Signal enhancement techniques for chromatography detection systems

The Environmental Protection Act has created a growing need for the measurement and assessment of trace emissions to the environment. This encompasses three main areas of ground, water and the atmosphere. The need to achieve lower emissions has placed a large burden on analytical techniques, particularly in the areas of trace analysis to ppb and ppt levels. Chromatographic techniques are widely used for assessment and measurement of emissions in all three areas. Enhanced detectors using mass spectrometry principles are available to lower detection limits, but these are expensive. Standard chromatography detectors can be used for trace analysis, but this often leads to extensive sample preparation stages to achieve low detection limits. This paper describes the techniques developed by Thomas Swan & Company to introduce a cost effective way of lowering detection limits. The approach taken meets both BATNEEC and BPEO constraints

The Dzhungarian fault: Late Quaternary tectonics and slip rate of a major right-lateral strike-slip fault in the northern Tien Shan region

The Dzhungarian strike-slip fault of Central Asia is one of a series of long, NW-SE right-lateral strike-slip faults that are characteristic of the northern Tien Shan region and extends over 300 km from the high mountains into the Kazakh Platform. Our field-based and satellite observations reveal that the Dzhungarian fault can be characterized by three 100 km long sections based on variation in strike direction. Through morphological analysis of offset streams and alluvial fans, and through optically stimulated luminescence dating, we find that the Dzhungarian fault has a minimum average late Quaternary slip rate of 2.2 ± 0.8 mm/yr and accommodates N-S shortening related to the India-Eurasia collision. This shortening may also be partly accommodated by counterclockwise rotation about a vertical axis. Evidence for a possible paleo-earthquake rupture indicates that earthquakes up to at least Mw 7 can be associated with just the partitioned component of reverse slip on segments of the central section of the fault up to 30 km long. An event rupturing longer sections of the Dzhungarian fault has the potential to generate greater magnitude earthquakes (Mw 8); however, long time periods (e.g., thousands of years) are expected in order to accumulate enough strain to generate such earthquakes.We thank the Royal Society International Travel Grant, Mike Coward Fund of the Geological Society of London, Percy Sladen Fund of the Linnean Society, The Gilchrist Educational Trust, and the Earth and Space Foundation for their support in funding this project. GEC’s doctoral studentship is funded by the National Environmental Research Council through NCEO, COMET, and the NERC-ESRC funded Earthquakes without Frontiers (EWF) Project. RTW is supported by a University Research Fellowship awarded by the Royal Society.This is the final version of the article, originally published in the Journal of Geophysical Research: Solid Earth. It is also available from Wiley at http://onlinelibrary.wiley.com/doi/10.1002/jgrb.50367/abstract. © 2013. American Geophysical Unio

Characterization of acyl chain position in unsaturated phosphatidylcholines using differential mobility-mass spectrometry

Glycerophospholipids (GPs) that differ in the relative position of the two fatty acyl chains on the glycerol backbone (i.e., sn-positional isomers) can have distinct physicochemical properties. The unambiguous assignment of acyl chain position to an individual GP represents a significant analytical challenge. Here we describe a workflow where phosphatidylcholines (PCs) are subjected to ESI for characterization by a combination of differential mobility spectrometry and MS (DMS-MS). When infused as a mixture, ions formed from silver adduction of each phospholipid isomer {e.g., [PC (16:0/18:1) + Ag]+ and [PC (18:1/16:0) + Ag]+} are transmitted through the DMS device at discrete compensation voltages. Varying their relative amounts allows facile and unambiguous assignment of the sn-positions of the fatty acyl chains for each isomer. Integration of the well-resolved ion populations provides a rapid method (\u3c 3 min) for relative quantification of these lipid isomers. The DMS-MS results show excellent agreement with established, but time-consuming, enzymatic approaches and also provide superior accuracy to methods that rely on MS alone. The advantages of this DMS-MS method in identification and quantification of GP isomer populations is demonstrated by direct analysis of complex biological extracts without any prior fractionation

Excitation spectrum of a two-component Bose-Einstein condensate in a ring potential

A mixture of two distinguishable Bose-Einstein condensates confined in a ring potential has numerous interesting properties under rotational and solitary-wave excitation. The lowest-energy states for a fixed angular momentum coincide with a family of solitary-wave solutions. In the limit of weak interactions, exact diagonalization of the many-body Hamiltonian is possible and permits evaluation of the complete excitation spectrum of the system.Comment: 4 pages, 1 figur

Structure of the regulatory hyaluronan binding domain in the inflammatory leukocyte homing receptor CD44

Adhesive interactions involving CD44, the cell surface receptor for hyaluronan, underlie fundamental processes such as inflammatory leukocyte homing and tumor metastasis. Regulation of such events is critical and appears to be effected by changes in CD44 N-glycosylation that switch the receptor "on" or "off" under appropriate circumstances. How altered glycosylation influences binding of hyaluronan to the lectin-like Link module in CD44 is unclear, although evidence suggests additional flanking sequences peculiar to CD44 may be involved. Here we show using X-ray crystallography and NMR spectroscopy that these sequences form a lobular extension to the Link module, creating an enlarged HA binding domain and a formerly unidentified protein fold. Moreover, the disposition of key N-glycosylation sites reveals how specific sugar chains could alter both the affinity and avidity of CD44 HA binding. Our results provide the necessary structural framework for understanding the diverse functions of CD44 and developing novel therapeutic strategies

Palliative care needs in patients hospitalized with heart failure (PCHF) study: rationale and design

Abstract Aims The primary aim of this study is to provide data to inform the design of a randomized controlled clinical trial (RCT) of a palliative care (PC) intervention in heart failure (HF). We will identify an appropriate study population with a high prevalence of PC needs defined using quantifiable measures. We will also identify which components a specific and targeted PC intervention in HF should include and attempt to define the most relevant trial outcomes. Methods An unselected, prospective, near-consecutive, cohort of patients admitted to hospital with acute decompensated HF will be enrolled over a 2-year period. All potential participants will be screened using B-type natriuretic peptide and echocardiography, and all those enrolled will be extensively characterized in terms of their HF status, comorbidity, and PC needs. Quantitative assessment of PC needs will include evaluation of general and disease-specific quality of life, mood, symptom burden, caregiver burden, and end of life care. Inpatient assessments will be performed and after discharge outpatient assessments will be carried out every 4 months for up to 2.5 years. Participants will be followed up for a minimum of 1 year for hospital admissions, and place and cause of death. Methods for identifying patients with HF with PC needs will be evaluated, and estimates of healthcare utilisation performed. Conclusion By assessing the prevalence of these needs, describing how these needs change over time, and evaluating how best PC needs can be identified, we will provide the foundation for designing an RCT of a PC intervention in HF

Can we use medical examiners' records for suicide surveillance and prevention research in Nova Scotia?

Introduction: Medical examiners' records can contribute to our understanding of the extent of suicide in a population, as well as associated sociodemographic and other factors

Optical properties of carbon nanofiber photonic crystals

Carbon nanofibers (CNF) are used as components of planar photonic crystals. Square and rectangular lattices and random patterns of vertically aligned CNF were fabricated and their properties studied using ellipsometry. We show that detailed information such as symmetry directions and the band structure of these novel materials can be extracted from considerations of the polarization state in the specular beam. The refractive index of the individual nanofibers was found to be n_CNF = 4.1.Comment: 10 pages, 4 figure

Concentration Dependence of the Effective Mass of He-3 Atoms in He-3/He-4 Mixtures

Recent measurements by Yorozu et al. (S. Yorozu, H. Fukuyama, and H. Ishimoto, Phys. Rev. B 48, 9660 (1993)) as well as by Simons and Mueller (R. Simons and R. M. Mueller, Czhechoslowak Journal of Physics Suppl. 46, 201 (1976)) have determined the effective mass of He-3 atoms in a He-3/He-4 mixture with great accuracy. We here report theoretical calculations for the dependence of that effective mass on the He-3 concentration. Using correlated basis functions perturbation theory to infinite order to compute effective interactions in the appropriate channels, we obtain good agreement between theory and experiment.Comment: 4 pages, 1 figur