6 research outputs found
Ocorrência do primeiro caso autóctone canino por Leishmania (Leishmania) infantum chagasi no Município de Campinas, Estado de São Paulo, Brasil
An autochthonous case of visceral leishmaniasis is reported in a dog (Canis familiaris) as an apparently natural infection in a non-endemic area. DNA obtained from spleen and liver samples produced the expected fragment in a Leishmania-specific rDNA-based nested-PCR assay. The PCR product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of Leishmania (Leishmania) infantum chagasi. These results are surprising since no autochthonous human or canine cases of visceral leishmaniasis have ever been reported in this municipality. This case suggests that natural transmission of this disease is occurring in this area.Caso autóctone de leishmaniose visceral é relatado em cão (Canis familiaris), aparentemente em área não endêmica. DNA obtido a partir de amostras do baço e fígado foram submetidos a nested-PCR baseada no rDNA específico de Leishmania. Os produtos das PCR foram sequenciados e os 490 pares de base foram idênticos a Leishmania (Leishmania) infantum chagasi. Esses resultados são surpreendentes, uma vez que, nenhum caso autóctone canino ou humano de leishmaniose visceral havia sido relatado neste município. Esse caso sugere que a transmissão natural da doença está ocorrendo nesta área
First occurrence of an autochthonous canine case of Leishmania (Leishmania) infantum chagasi in the municipality of Campinas, State of São Paulo, Brazil
SHORT COMMUNICATION - Ocurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil
A natural case of co-infection by Leishmania and Trypanosoma is
reported in a dog (Canis familiaris ) in south- western state
of Mato Grosso do Sul, Brazil. Both amastigote and trypomastigote forms
were observed after Giemsa staining of cytological preparations of the
dog's bone marrow aspirate. No parasite was detected using medium
culture inoculation of the sample. DNA obtained from the bone marrow
aspirate sample and from the blood buffy coat was submitted to
polymerase chain reaction (PCR) with a set of rDNA-based primers
S4/S12. The nucleotide sequence of the PCR product was identical to
that of Trypanosoma (Trypanozoon) evansi . The S4/S12 PCR was
then used as template in a nested-PCR using a specific Leishmania set
S17/S18 as primers, to explain the amastigote forms. The nucleotide
sequence of the new PCR product was identical to that of Leishmania
(Leishmania) chagasi . This case, as far as we know, is the
first report of a dog co-infected with these parasites, suggesting that
besides L. (L.) chagasi, the natural transmission of T. (T.) evansi
occurs in the area under study
Occurrence of canine visceral leishmaniasis in an agricultural settlement in the State of Mato Grosso do Sul, Brazil
Universidade para o Desenvolvimento do Estado e da Região do Pantanal. Centro de Ciências Agrárias, Biológicas e da Saúde. Campo Grande, MS, Brasil.Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia. São Paulo, SP, Brasil.Médicos VeterináriosMédicos VeterináriosPrefeitura do Município de São Paulo. Centro de Controle de Zoonoses. São Paulo, SP, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Prefeitura do Município de São Paulo. Centro de Controle de Zoonoses. São Paulo, SP, Brasil.Prefeitura do Município de São Paulo. Centro de Controle de Zoonoses. São Paulo, SP, Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Patologia. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Patologia. Campo Grande, MS, Brasil.Durante estudos sobre a fauna flebotomínea em assentamento do Instituto Nacional de Reforma
Agrária (INCRA) na Serra da Bodoquena, Estado de Mato Grosso do Sul, Brasil, foram observados cães com
manifestações clínicas sugestivas de leishmaniose visceral. Inquérito sorológico para leishmaniose em 97
cães, utilizando reação de imunofluorescência indireta, mostrou 23 (23,7%) soros reagentes. Amostras do
parasita foram identificadas como Leishmania (Leishmania) chagasi.During previous research on phlebotomine fauna in a settlement of the Brazilian National Agrarian
Reform Institute (INCRA) on the Bodoquena Range, Mato Grosso do Sul State, Brazil, dogs were observed
with clinical aspects suggestive of visceral leishmaniasis. A serological survey to leishmaniasis in 97 dogs, by
indirect immunofluorescence test, showed 23 (23.7%) serum positive dogs. Samples of the parasites were
identified as Leishmania (Leishmania) chagasi