Rates and sources of phosphorus and mycorrhizal fungi on the nutrition ai the 'Rangpur' lime until transplanting

Com o objetivo de verificar o efeito de diferentes doses e fontes de fósforo e de fungos MVA sobre os teores de nutrientes do limoeiro 'Cravo', cultivado em sementeiras removíveis com substrato comercial até a repicagem foi conduzido um experimento em casa de vegetação, na Escola Superíor de Agricultura de Lavras (ESAL), MG. Adotou-se o delineamento de blocos casualizados em esquema de parcelas subdivididas. Foram testados, nas parcelas, os tratamentos inoculados com os fungos micorrízicos Acaulospora morrowae e Gbinus e/atum, e os não-inoculados. As subparcelas foram compostas pelos tratamentos de doses e fontes de P 205 em esquema fatorial, com dosagens de 0; 320; 640 e 1280 gramas de P206 por m3 de substrato, fornecidas através de superfosfato simples e apatita de Araxá. Doses cresoentes de superfosfato simples proporcionaram aumentos lineares nos teores de P e Ca, enquanto para a apatita de Araxá houve aumentos lineares para os teores de Fe e Mn. O fungo Acaubospora morrowae proporcionou altas taxas de colonizaçaó micorrízica nas raízes e aumentos na absorção de P, K e Zn pelas plantasThis research was carried out at the Escola Superior de Agricultura de Lavras (ESAL), in Lavras, MG, Brazil. The objective was to study the effect of diferente rates and sources of phosphorus and inoculation with vesicular-arbuscular mycorrhizae (VAM) on the nutrition of 'Rangpur' lime, growing in seedlings until transplanting. The experimental design used was randomized blocks in split- splot scheme. The plots were composed by the inoculation treatments with the mycorrhizal fungi Acaulospora morrowae, Glomus clarum and a noninoculated control. The sub-plots were composed by the rates and sources of P 205 in a factorial scheme with the rates of 0; 320; 640 and 1280 g of P205 by m3 of substrate and two sources of phosphorus fertilizers, the single superphosphate and the apatite of Araxá. Increasing the rates of single superphosphate, made higher concentration of P and Ca in the dry matter four months after sowing, in relation to use of apatite of Araxá. The fungus Acaulospora norrowae made higher rates of root colonization and higher absorption of P, K and Zn by the plant

Avaliação da deterioração em sementes de castanheira-do-brasil pelo teste de tetrazólio

The objective of this work was to establish coloration patterns by the tetrazolium test that allows evaluation of the physiological quality of the Brazilian nut seeds (Bertholletia excelsa Humb. & Bompl.). After 30 days of storage in laboratory environmental conditions, and thereafter, every 15 days, at amount of 105 days, the moisture content and the coloration patterns in the internal sections of kernels were evaluated. With the storage time there was a decrease in the moisture content and in the percentage of the kernels with pink color and an increase in the percentage of kernel with white blots. The white coloration tended do fill up all the internal sections of the kernel indicating that there is possibly an association with the evolution of water loss and deterioration of the seeds.O presente trabalho teve por objetivo estabelecer padrões de coloração mediante o teste de tetrazólio, que permitam avaliar a qualidade fisiológica de sementes de castanheira-do-brasil (Bertholletia excelsa Humb. & Bompl.). Trinta dias após o armazenamento sob condições ambientais de laboratório, e, posteriormente, a cada 15 dias, até um total de 105 dias, foram avaliados o grau de umidade e os padrões de coloração na secção interna das amêndoas. Com o tempo de armazenamento, houve diminuição no grau de umidade, na porcentagem de amêndoas com coloração rósea, e aumento na porcentagem de amêndoas com manchas brancas. A coloração branca tendeu a tomar toda a secção interna da amêndoa, o que indica evolução da perda de água e deterioração das sementes

Predictors of rescue percutaneous coronary intervention after pharmacoinvasive strategy in women

AbstractBackgroundPharmacoinvasive therapy (PIT) is feasible in patients with acute myocardial infarction with ST-segment elevation (STEMI) when timely primary percutaneous coronary intervention (PCI) is unavailable. In this study, we compared women who underwent successful reperfusion PIT with those who required rescue PCI, to identify potential predictors of thrombolytic failure.MethodsFrom January 2010 to November 2014, 327 consecutive women with STEMI were referred to a tertiary hospital, 206 after successful thrombolysis (63%) and 121 who required rescue PCI. The groups were compared regarding demographic, clinical and angiographic outcomes, and clinical (TIMI, GRACE, and ZWOLLE CADILLAC) and bleeding (CRUSADE) risk scores. A multivariate logistic regression model was used to identify predictors of thrombolytic failure.ResultsThere was no significant difference between the demographic characteristics or the medical history of the groups. Rescue PCI group had significantly higher values of the evaluated scores. Clinical hospital complications and mortality (2.5% vs. 22.0%; p < 0.0001) were more frequent in rescue PCI group. The independent variables associated with rescue PCI were pain-to-needle time > 3h (OR: 3.07, 95%CI: 1.64 to 5.75; p < 0.0001), ZWOLLE score (OR: 1.25; 95%CI: 1.14 to 1.37; p = 0.0001) and creatinine clearance (OR: 1.009, 95%CI: 1.0 to 1.02; p = 0.04).ConclusionsWomen with STEMI who underwent PIT and who required rescue PCI had significantly higher mortality compared to those who achieved initial success of PIT with elective PCI. Pain-to-needle time > 3h, ZWOLLE score and creatinine clearance were independent predictors of the need for rescue PCI

Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures

This study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (MIC) was measured for isolates and subpopulations growing on vancomycin screen agar. The mecA gene was detected in 81.3% of the S. epidermidis isolated and 48.2% carried SCCmec type III. The complete icaADBC operon was observed in 38.3% of the isolates; of these, 58.5% produced a biofilm. Furthermore, 47.7% of the isolates grew on vancomycin screen agar, with an increase in the MIC in 75.9% of the isolates. Determination of the MIC of subpopulations revealed that 64.7% had an MIC ≥ 4 μg mL-1, including 15.7% with an MIC of 8 μg mL-1 and 2% with an MIC of 16 μg mL-1. The presence of the icaADBC operon, biofilm production and reduced susceptibility to vancomycin were associated with methicillin resistance. This study reveals a high level of methicillin resistance, biofilm formation and reduced susceptibility to vancomycin in subpopulations of S. epidermidis. These findings may explain the selection of multidrug-resistant isolates in hospital settings and the consequent failure of antimicrobial treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Nitroisatin dithiocarbazate: Synthesis, structural characterization, DFT, and docking studies

The reaction between 5-nitroisatin with S-benzyl dithiocarbazate affords a new isatindithio carbazate so-called NO2Isadtc (Benzyl 2-(5-nitro-2-oxoindolin-3-ylidene)hydrazinecarbodi thioate) which was characterized by means of 1H NMR, FT-IR, UV-visible and single crystal X-ray diffraction - Crystal data for C16H12N4O3S2 (M =372.42 g/mol): triclinic space group P-1, (n°. 02), a = 6.640 Å, b = 8.256 Å, c = 15.908 Å, V = 849.6 Å3, Z = 2, T = 293 K, μ(MoKα) = 0.337 mm-1, Dcalc = 1.456 g/cm3, 27515 reflections measured (2.499° ≤ 2Θ ≤ 26.524°), 3518 unique (Rint = 0.0533, Rsigma =0.0222) which were used in all calculations. The final R1 was 0.0367 (I > 2σ(I)) and wR2 was 0.1045 (all data). Computational methods were applied to NO2Isadtc and its nonsubstituted parent compound Isadtc for structure optimization, electronic distribution, and infrared calculations using B3LYP functional with 6-31G(d,p) basis set in ethanol as a polarizable continuum model. Furthermore, docking studies using human thioredoxin reductase 1 (TrxR) as enzyme target also were performed using NO2Isadtc and the optimized structure of Isadtc. The results demonstrated that both NO2Isadtc and Isadtc may act as inhibitors of TrxR, having different interactions detected, highlighting the contact between the NO2 group and the S111 at the helix which is found for NO2Isadtc