713 research outputs found

    Primera demostración paleontológica del Kimmeridgiense en el Garraf (Barcelona)

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    El hallazgo de Pagiophyllum cirinicum (SAPORTA), (Cofiferales), en la parte inferior de las dolomias de Garraf confirma la cronostratigrafia  kimmeridgiense de la mayor parte de tales dolomias Se ofrece además una visión estratigráfica de todo el conjunto

    Structure of a bacterial type IV secretion core complex at subnanometre resolution

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    Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived

    Superintegrability and higher order polynomial algebras II

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    In an earlier article, we presented a method to obtain integrals of motion and polynomial algebras for a class of two-dimensional superintegrable systems from creation and annihilation operators. We discuss the general case and present its polynomial algebra. We will show how this polynomial algebra can be directly realized as a deformed oscillator algebra. This particular algebraic structure allows to find the unitary representations and the corresponding energy spectrum. We apply this construction to a family of caged anisotropic oscillators. The method can be used to generate new superintegrable systems with higher order integrals. We obtain new superintegrable systems involving the fourth Painleve transcendent and present their integrals of motion and polynomial algebras.Comment: 11 page

    Native Plants to Arid Areas: A Genetic Reservoir for Drought-Tolerant Crops

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    Droughts are common in arid areas. These cause important losses in crop production, while the increasing population demands more food and goods. Cultivars able to produce under drought conditions are required to avoid or reduce production losses. Plants have evolved different mechanisms to face drought, and many genes have been already discovered in model and cultivated plants that are involved in this trait. Some of these genes have been successfully transformed into cultivated plants for drought tolerance. Plants native to arid lands may possess variants of drought tolerance mechanisms as compared to mesophytic or model plants. Also, different drought-related genes can be revealed. Studies using high-throughput and bioinformatic tools may allow to discover new genes and give new insights on the mechanisms involved in drought tolerance. However, still scarce studies in plants native to arid lands show that there are many drought-related genes that have not been already characterized and potentially they may be novel genes. These novel genes may be used to improve crops for drought tolerance. Therefore, more physiological, transcriptomic, proteomic, and metabolomic studies are needed on plants native to the deserts

    CHR11, a chromatin-remodeling factor essential for nuclear proliferation during female gametogenesis in Arabidopsis thaliana

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    Chromatin-remodeling factors regulate the establishment of transcriptional programs during plant development. Although 42 genes encoding members of the SWI2/SNF2 family have been identified in Arabidopsis thaliana, < 10 have been assigned a precise function on the basis of a mutant phenotype, and none have been shown to play a specific role during the gametophytic phase of the plant life cycle. A. thaliana chromatin-remodeling protein 11 (CHR11) encodes an imitation of switch (ISWI)-like chromatin-remodeling protein abundantly expressed during female gametogenesis and embryogenesis in Arabidopsis. To determine the function of CHR11 in wild-type plants, we introduced a hairpin construct leading to the production of double-stranded RNA, which specifically degraded the endogenous CHR11 mRNA by RNA interference (RNAi). Transcription of the RNAi-inducing hairpin RNA was driven by either a constitutive cauliflower mosaic virus 35S promoter (CaMV35S) acting at most stages of the sporophytic phase or a newly identified specific promoter acting at the onset of the female gametophytic phase (pFM1). All adult trans-formants that constitutively lacked sporophytic CHR11 activity showed reduced plant height and small cotyledonary embryos with limited cell expansion. In contrast, RNAi lines in which CHR11 was specifically silenced at the onset of female gametogenesis (megagametogenesis) had normal height and embryo size but had defective female gametophytes arrested before the completion of the mitotic haploid nuclear divisions. These results show that CHR11 is essential for haploid nuclear proliferation during megagametogenesis and cell expansion during the sporophytic phase, demonstrating the functional versatility of SW12/SNF2 chromatin-remodeling factors during both generations of the plant life cycle

    A numerical approach to analyze the performance of a PEF-Ohmic heating system in microbial inactivation of solid food

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    Pulsed Electric Fields (PEF) technology has been recently proposed as a new ohmic-heating system for the heat treatment of solid products in short periods (less than 1 min). However, similar to traditional ohmic heating, non-homogeneous distribution of temperature has been observed and cold points appeared in the interphase between the solid treated product and the electrodes, which can limit the technology for assuring food safety for treated solid products. In this investigation, a computational axisymmetric model of a lab-scale PEF system for a solid product (agar cylinder) was developed. This model was used to predict the temperature and the electric field distribution, treatment time, and the microbial inactivation (Salmonella Typhimurium 878) in the solid product after a PEF-ohmic treatment. Using a factorial analysis, a total of 8 process conditions with different settings of applied field strength levels (2.5–3.75 kV/cm), frequencies (100–200 Hz), and initial agar and electrode temperature (40–50°C) were simulated for the agar cylinder in order to identify the effect and optimal values of these parameters, which offer the most temperature homogeneity. The results showed that the initial temperature of the agar and the electrodes was of great importance in achieving the best temperature uniformity, limiting the occurrence of cold points, and therefore, improving the homogeneity in the level of inactivation of Salmonella Typhimurium 878 all over the agar cylinder. A treatment of 2.3 s would be enough at 3.75 kV/cm, 200 Hz with an initial temperature of 50°C of the agar and the electrodes, for a 5-Log10 reduction of Salmonella Typhimurium 878 in the whole product with a deviation of 9°C between the coldest and hottest point of the solid

    Superintegrable systems with spin and second-order integrals of motion

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    We investigate a quantum nonrelativistic system describing the interaction of two particles with spin 1/2 and spin 0, respectively. We assume that the Hamiltonian is rotationally invariant and parity conserving and identify all such systems which allow additional integrals of motion that are second order matrix polynomials in the momenta. These integrals are assumed to be scalars, pseudoscalars, vectors or axial vectors. Among the superintegrable systems obtained, we mention a generalization of the Coulomb potential with scalar potential V0=αr+328r2V_0=\frac{\alpha}{r}+\frac{3\hbar^2}{8r^2} and spin orbital one V1=2r2V_1=\frac{\hbar}{2r^2}.Comment: 32 page

    Management Practices and Bioproductivity in Grassland of Dry Areas

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    In Mexico, the grassland represents 40.1% of the total area of the country and it is a source of feed for livestock, although suffers different degrees of degradation due to lack of management and adverse climatic conditions. The problem of the grasslands is complex since it involves diverse type’s soils, presence of invasive plants, low success in the establishment of grasses or replanting, high fluctuation in the rainfall distribution, as well as the low capacity of the soil to retain moisture. Among these constraints, the limited availability of soil moisture in arid conditions, makes these areas more fragile to the degradation of the environment which results in low productivity of the grassland. In this chapter, major ecological limitations of the grassland and techniques which improve the soils moisture retention capacity of the grassland especially in moisture deficit areas will be discussed

    Water Harvesting and Soil Water Retention Practices for Forage Production in Degraded Areas in Arid Lands of Mexico

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    The area under arid conditions in Mexico is greater than 50%. This area faces a high risk due to environmental effects. The soil degradation in arid, semi‐arid, and dry sub‐humid areas is of multi‐causal nature, among which climatic and anthropogenic factors stand out. At least, three distinct elements with different effects may be considered: recurrent droughts in short periods, long‐term climate fluctuations, and degradation of soils by human activities. These threaten the productivity and sustainability of ecosystems and agro‐ecosystems. Thus, it is needed to maintain a constant exploration of new and more appropriate technologies that promote the efficient use of natural resources, in a framework of greater sustainability. Many of these technologies are focused toward better management of water and soil resources in production systems. Water management is oriented with rainwater harvesting, efficient irrigation systems, as well as soil moisture retention techniques, and the use of plant species tolerant to water stress. Planting of native species and using soil improvers of edaphic moisture retention can enhance reclamation (recovery) of degraded soils. The aim of this chapter is to show and discuss some experimental results using the above technologies applied to rangelands with degraded soils in dry lands

    XANES and EXAFS study of the local order in nanocrystalline yttria-stabilized zirconia

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    The local order around Zr and Y atoms of nanocrystalline yttria-stabilized zirconia (YSZ) powders with different grain sizes has been investigated by x-ray absorption spectroscopies. The samples were prepared by means of mechanical alloying with or without subsequent sintering treatment and also by milling commercial YSZ. Our study is motivated by the interest in the electrical properties of grain boundaries and the controversy about the level of disorder in the intergrain regions in nanocrystalline YSZ. The x-ray absorption near edge structure (XANES) analysis indicates that the local order of all the sintered samples is independent of the grain size. This is confirmed by the analysis of the extended x-ray absorption fine structure, which points out also that, in contrast to that found in sintered samples, the local order around the cation in the samples milled without further sintering treatment extends only to the first coordination shell. Finally, the results of ab initio Zr K-edge XANES calculations lead us to conclude that the observed changes of the shape of the white line are not related to a phase transformation but reflects the short-range order present in the as-milled samples
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