21 research outputs found
Simultaneous occurrence of cerebellar medulloblastoma and pituitary adenoma: A case report
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Is there any potential link among caspase-8, p-p38 MAPK and bcl-2 in clear cell renal cell carcinomas? A comparative immunohistochemical analysis with clinical connotations
Abstract Background Clear cell renal cell carcinomas (ccRCCs) constitute the most common renal carcinomas, characterized by a relatively aggressive clinical course. Thus, scientific research is targeting towards the identification of immunohistochemical and molecular markers that could be useful regarding diagnosis, appropriate therapy and prediction of prognosis. In the present study we assessed and correlated the expression of caspase-8, phosphorylated p38 mitogen-activated protein kinase (p-p38) and bcl-2 protein with histopathological features and clinical outcome of 27 patients with ccRCCs. Method Immunohistochemistry in formalin-fixed and paraffin-embedded tissue sections was performed. The associations among various features were assessed utilizing statistical analysis. Results We found that increased expression of cytoplasmic caspase-8 and bcl-2 protein was strongly associated with low Fuhrman's grade of carcinomas (p = 0.019 and p = 0.041, respectively). On the other hand, increased p-p38 expression was significantly related to high Fuhrman's grade (p = 0.006). Moreover, high bcl-2 expression was correlated with low pathological stage of ccRCCs (p = 0.026). Increased expression of cytoplasmic caspase-8 as well as low-grade tumors (grade 1 and 2) implied a greater probability of patients' survival, in univariate statistical analysis (p = 0.037 and p = 0.019, respectively). Neither p-p38 nor bcl-2 expression was significantly linked to patients' survival. There were not emerged statistically significant associations among caspase-8, p-p38 kinase and bcl-2 protein. Conclusion For the first time the prognostic impact of caspase-8 and p-p38 was studied in a series of ccRCCs, using immunohistochemistry in formalin-fixed and paraffin-embedded tissue sections. The suggestive relationship of caspase-8 with patients' clinical outcome, as well as the role of p-p38 within different grade categories, mandates further studies in larger cohorts of RCCs.</p
Is there any potential link among caspase-8, p-p38 MAPK and bcl-2 in clear cell renal cell carcinomas? A comparative immunohistochemical analysis with clinical connotations
10.1186/1746-1596-4-7Diagnostic Pathology41
Is there any potential link among caspase-8, p-p38 MAPK and bcl-2 in clear cell renal cell carcinomas? A comparative immunohistochemical analysis with clinical connotations
Background: Clear cell renal cell carcinomas (ccRCCs) constitute the
most common renal carcinomas, characterized by a relatively aggressive
clinical course. Thus, scientific research is targeting towards the
identification of immunohistochemical and molecular markers that could
be useful regarding diagnosis, appropriate therapy and prediction of
prognosis. In the present study we assessed and correlated the
expression of caspase-8, phosphorylated p38 mitogen-activated protein
kinase (p-p38) and bcl-2 protein with histopathological features and
clinical outcome of 27 patients with ccRCCs.
Method: Immunohistochemistry in formalin-fixed and paraffin-embedded
tissue sections was performed. The associations among various features
were assessed utilizing statistical analysis.
Results: We found that increased expression of cytoplasmic caspase-8 and
bcl-2 protein was strongly associated with low Fuhrman’s grade of
carcinomas (p = 0.019 and p = 0.041, respectively). On the other hand,
increased p-p38 expression was significantly related to high Fuhrman’s
grade (p = 0.006). Moreover, high bcl-2 expression was correlated with
low pathological stage of ccRCCs (p = 0.026). Increased expression of
cytoplasmic caspase-8 as well as low-grade tumors (grade 1 and 2)
implied a greater probability of patients’ survival, in univariate
statistical analysis (p = 0.037 and p = 0.019, respectively). Neither
p-p38 nor bcl-2 expression was significantly linked to patients’
survival. There were not emerged statistically significant associations
among caspase-8, p-p38 kinase and bcl-2 protein.
Conclusion: For the first time the prognostic impact of caspase-8 and
p-p38 was studied in a series of ccRCCs, using immunohistochemistry in
formalin-fixed and paraffin-embedded tissue sections. The suggestive
relationship of caspase-8 with patients’ clinical outcome, as well as
the role of p-p38 within different grade categories, mandates further
studies in larger cohorts of RCCs
High prevalence of Human Herpes Virus 8 (HHV-8) in patients with Warthin\u27s tumors of the salivary gland
Background: Warthin\u27s tumor is a common benign neoplasm of the salivary gland. Human Herpes Virus 8 (HHV-8) is the etiologic agent for all forms of Kaposi\u27s sarcoma (KS), and HHV-8 DNA is present in saliva, suggesting that non-sexual transmission is associated with latent infection of the salivary gland. Objectives: To provide insights into the HHV-8 cell tropism, the presence of HHV-8 was investigated in a series of Warthin\u27s tumors of the salivary gland and corresponding adjacent normal tissue. Study design: Forty-three patients with Warthin\u27s tumors (cystadenolymphoma) were tested for the presence of HHV-8 DNA, and corresponding adjacent normal tissue samples were obtained from 15 patients. DNA was extracted from the paraffin-embedded tissues. A nested polymerase chain reaction (PCR) assay was applied, and the positive samples were confirmed by direct sequencing. Results: HHV-8 DNA was detected in 19 out of 43 (44%) salivary gland tumor samples. Among the 15 cases with paired samples, 9 were HHV-8-positive for both samples, 4 were HHV-8-negative for both samples while in two cases HHV-8 was detected only in the tumor specimens. Conclusions: HHV-8 is frequently detected in adenoid salivary neoplasms, suggesting a significant role of the virus in the etiopathogenesis of the disease. Larger studies are required to investigate the role of HHV-8 in the development or progression of Warthin\u27s tumors
Treatment with Molgramostim (Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor, Rhugm-Csf, Mielogen) and Lenograstim (Granulocyte-Colony Stimulating Factor) Improves Experimental Colitis in Rats
Background/Aim. Treatment with growth factors could be beneficial in
both inflammatory bowel disease and experimental colitis. The aim of
this study was to investigate the effect of Colony Stimulating Factor
(CSF), and Recombinant Human (rHu) Granulocyte Stimulating Factor (GSF)
in experimental colitis in rats. Methods. Experimental colitis was
induced in 62 male Wistar rats, divided into 9 groups, using
2,4,6-trinitrobenzensulfonic acid (TNBS). Group 1: Ten rats with colitis
without treatment (control group). Euthanasia after 15 days. Group 2:
Ten animals with colitis without treatment (control group). Euthanasia
after 30 days. Group 3: Six animals with colitis. Immediate treatment
with CSF. Euthanasia after 19 days. Group 4: Six animals with colitis.
Treatment started 7 days after the induction of colitis. Animals were
kept for 19 days. Group 5: Six animals with colitis. Treatment started 2
weeks after the induction of colitis. Group 6: Six animals with colitis,
the same as in group 3. Treatment with GSF. Group 7: Six animals with
colitis, the same as in group 4. Treatment with GSF. Group 8. Six
animals with colitis, the same as in group 5. Treatment with GSF. Group
9: Six animals with colitis. Immediate treatment with prednisolone.
Euthanasia after 15 days. Results. CSF and GSF administration
significantly improved the histological score (P < 0.05) and reduced
malondialdehyde contents (P < 0.05), compared to control groups in all
animals. CSF was superior to GSF and to prednisolone. Conclusion.
Administration of both CSF and GSF could significantly improve the
histological score and oxidative stress in experimental colitis in rats