Effect of anticoagulants on plasma homocysteine determination

Background: Waiting temperature before centrifugation and anticoagulants used, markedly effect total homocysteine concentrations. The aim of this study was to investigate the effect of different anticoagulants and temperature on plasma homocysteine levels. Methods: We studied total homocysteine concentrations in 23 healthy subjects. Blood was drawn in K(3)EDTA, sodium citrate- or sodium fluoride-containing tubes, and kept at 0 degreesC or 22 degreesC for 3 h. Total homocysteine measurements were performed with fluorescence polarization immunoassay (FPIA) method. We compared all results with baseline EDTA values (samples put on crushed ice and centrifuged immediately) recommended in literature for reference handling. Results: At 22 degreesC, the tubes: containing sodium citrate and sodium fluoride showed significantly higher total homocysteine concentrations than their respective baseline values (p = 0.000). However, sodium fluoride tubes were not significantly different than baseline EDTA levels. Waiting 3 h at 0 degreesC did not effect sodium citrate and EDTA plasma total homocysteine concentrations when compared to baseline EDTA, but sodium fluoride-containing plasma levels were significantly decreased( p = 0.000). Conclusions: According to our results, the most available and practical temperature and anticoagulant for total homocysteine determination is sodium fluoride at room temperature up to 3 h. (C) 2001 Elsevier Science B.V. All rights reserved

The role of enalapril in the prevention of ovarian hyperstimulation syndrome: a rabbit model

The purpose of our study was to investigate the role of enalapril in the prevention of ovarian hyperstimulation syndrome. METHODS: Twenty New Zealand female rabbits were included in the study. A total of 75 IU FSH + 75 IU LH was given daily by i.m. route for the first 7 days and additionally 2500 IU HCG was given on the last day of ovarian stimulation. Between days 0 and 9, oral enalapril tablets (2 mg/kg) were given twice daily to 10 rabbits (group 1). The remaining 10 rabbits did not receive enalapril (group 2). Laparatomy was performed on all rabbits at day 9. The amount of peritoneal fluid and the weight of the ovaries were recorded during laparotomy. Serum renin, interleukin-6 (IL-6), oestradiol, progesterone, prolactin and aldosterone concentrations were assayed at day 0 and again at day 9 for all rabbits. RESULTS: Serum renin and IL-6 concentrations at day 9 increased significantly compared with basal values in both groups (P < 0.05). Renin was correlated with IL-6 at day 9 in both groups (P < 0.05). The amount of peritoneal fluid and the increase in body weight observed at day 9 were not significantly different between groups I and 2. Administration of enalapril did not prevent the formation of ascites in group I despite the low serum aldosterone concentrations. In group I the weight of ovaries was significantly higher than the control group (P < 0.05). CONCLUSIONS: Renin-angiotensin system and IL-6 may play a role in the aetiopathogenesis of ovarian hyperstimulation syndrome. Administration of enalapril did not seem to have any beneficial effect in reducing the severity of ovarian hyperstimulation syndrome

Urinary stone disease in diabetes mellitus

Objective: Diabetes mellitus (DM) and urinary stone disease (USD) are common diseases in the community. The prevalence of USD has not previously been studied in DM patients

Changes in oral health-related quality of life after treatment of molar incisor hypomineralisation using Glass Hybrid Restorations

Objective: To assess the changes in children\"s oral health-related quality of life following the treatment of severely affected molar-incisor hypomineralisation with Glass Hybrid Restorative System (GH) after selective caries removal. Method: The observational cross-sectional study was conducted at the Marmara University, School of Dentistry, Department of Pediatric Dentistry, Istanbul, Turkey.. Children aged 11-14 years (n = 55) who were diagnosed with MIH and had finished their dental treatment from November 2018 to December 2019, were included. The children\"s MIH-affected teeth were treated with GH after SCR. Participants answered the Child Perceptions Questionnaire (CPQ11?14) prior to their dental treatment and 6 months after the treatment. Results: Of the fifty-five patients, 40 patients (24 girls-16 boys) completed baseline and follow-up data. The mean age of the children was 11.85 +/- 1.02 years. The overall CPQ score ranged from 3-83 (average 33.27 +/- 16.46) at baseline and 0-61 (average 11.67 +/- 11.21) at follow up. The emotional well-being among children was the highest score at baseline. A significant decrease (p < 0.001) in the mean values was observed for both the overall CPQ scores and for the scores of the oral symptoms, functional limitations, and social-emotional well-being limitation. All subdomains showed large effect sizes and oral symptom limitation domain presented the greatest effect. Wilcoxon Rank test was used to determine the statistical significance of the changes and the magnitude of change was determined by calculating and classifying the effect size. Conclusion: Restorative treatment with GH following selective caries removal positively influenced the oral health -related quality of life of children with severe molar-incisor hypomineralisation

Raloxifene increases serum leptin levels in postmenopausal women: a prospective study.

Objective: The purpose of this study was to investigate the effect of raloxifene on leptin and insulin-like growth factor-I levels and their relation with the biochemical markers of bone metabolism in postmenopausal women

Longitudinal investigation of the relationship between breast milk leptin levels and growth in breast-fed infants

Background. It has been shown that leptin is present in breast milk and human mammary epithelial cells are able to synthesize leptin. It has been suggested that leptin in human milk might be involved in the regulation of postnatal nutrition and growth

Effect of clozapine on serum leptin, insulin levels, and body weight and composition in patients with schizophrenia

Objective: Weight gain frequently occurs during treatment with clozapine. However, the pathophysiology of clozapine-induced weight gain remains unclear. The aim of this study was to investigate the influence of clozapine on hormones leptin and insulin in relation to body weight and composition measures to determine their contribution to clozapine-induced weight gain. Method: Data are reported on 19 patients with schizophrenia (11 women and 8 men) who completed 10 weeks of treatment with clozapine. Insulin levels, weight measurements, body mass index (BMI), and body composition measurements were evaluated at baseline and at the end of treatment. Leptin levels were assessed at baseline and after 4 and 10 weeks of treatment. Analysis of variance with repeated measures was used to evaluate changes in weight, body composition measures, leptin, and insulin. The Pearson correlations were used to assess the relationships between changes in hormone levels and weight along with body composition measurements. The correlations of change in Positive and Negative Syndrome Scale (PANSS) score with changes in hormone levels, weight gain and body composition measures were evaluated with Pearson correlations. Results: Leptin and insulin levels did not show any significant alterations across time. The use of clozapine was associated with significant increases in BMI (F = 19.8, P < .001), lean muscle mass (F = 8.2, F = .01), and fat mass (F = 15.4, F = 001), while total body fluid percentage (F = 4.1, P = .05) significantly decreased. Improvement in PANSS scores was not correlated to change in leptin, insulin, weight, BMI, or body composition measurements. The change in leptin levels was correlated to change in body fat mass. Conclusion: The role of leptin in weight gain induced by clozapine might be a regulatory mechanism rather than being etiologic. (C) 2003 Elsevier Science Inc. All rights reserved

Regression of endometrial explants in rats treated with the cyclooxygenase-2 inhibitor rofecoxib

Objective: To investigate the effects of cyclooxygenase-2 (COX-2) inhibitor rofecoxib on endometrial explants and on peritoneal vascular endothelial growth factor (VEGF) levels in the rat endometriosis model