Presence of Class I and Class II Integrons in Methicilin Resistant Staphylococci and Their Relations with Antibiotic Resistance: A Preliminary Study from Turkey

Background: Emergence of antibiotic resistance is a major public health concern. It is known that antibiotic resistance is transferred by different ways. Integrons as one of these mechanisims cause to spread antibiotic resistance in Gram negative bacteria but also it is shown to be effective for transferring genes in Gram positive bacteria. In the present study we aimed to examine the prevalence of class I and class II integrons in MRSA and MRCNS strains isolated from patients and to determine the relationship between antibiotic resistance and the presence of integrons. Methods: Sixty four MRSA and 62 MRCNS strains were included in this study. Antibiotic susceptibility testings were performed. Genomic and plasmid DNAs were extracted and Polymerase Chain Reaction (PCR) was used for the detection of the intI and intII genes. The PCR products were visualised in 1.5 % agarose gel electrophoresis. Pearson chi-square test and Fisher’s exact test were used for comparing categorical variables. Results: Among 126 staphylococci 11 (8.7% - 4 MRSA and 7 MRCNS) were shown to carry class I integron; whereas 7 MRCNS (5.5%) were class II positive. Both of class I and class II integrons were detected to possess in four MRSA (3.2%). There was no statistically significant relation between presence of integrons and resistance to each of antibiotics (p>0.05). Conclusion & Recommendation: In the present study we did not find any significant relation between resistance rates and the presence of integrons but we suggest that these results showed an important data about the extended distributions of integrons not only among Gram negative bacteria but also in staphylococci.Keywords: MRSA, MRCNS, class I integron, class II integron, anbiotic resistanceDOI: 10.7176/JHMN/75-0

Voice Analysis to Differentiate the Dopaminergic Response in People With Parkinson's Disease

Humans' voice offers the widest variety of motor phenomena of any human activity. However, its clinical evaluation in people with movement disorders such as Parkinson's disease (PD) lags behind current knowledge on advanced analytical automatic speech processing methodology. Here, we use deep learning-based speech processing to differentially analyze voice recordings in 14 people with PD before and after dopaminergic medication using personalized Convolutional Recurrent Neural Networks (p-CRNN) and Phone Attribute Codebooks (PAC). p-CRNN yields an accuracy of 82.35% in the binary classification of ON and OFF motor states at a sensitivity/specificity of 0.86/0.78. The PAC-based approach's accuracy was slightly lower with 73.08% at a sensitivity/specificity of 0.69/0.77, but this method offers easier interpretation and understanding of the computational biomarkers. Both p-CRNN and PAC provide a differentiated view and novel insights into the distinctive components of the speech of persons with PD. Both methods detect voice qualities that are amenable to dopaminergic treatment, including active phonetic and prosodic features. Our findings may pave the way for quantitative measurements of speech in persons with PD

MOTOR DEVELOPMENT OUTCOMES OF CHILDREN WHO HAVE UNDERGONE THERAPEUTIC HYPOTHERMIA: WITH PARENTS' VIEWS

Objective: Neurodevelopmental follow-up of infants with hypoxic ischemic encephalopathy (HIE) and supporting their development have great importance for the later years of their life. The aim of this study was to evaluate motor development outcomes of children with HIE who have undergone therapeutic hypothermia (TH) in Turkey with an objective assessment and the point of view of the parents, and to compare these two assessment methods

Renal transplantation in a patient with chronic granulomatous disease: case report.

Chronic granulomatous disease (CGD) is a genetic disease caused by structural mutations in the enzyme NADPH oxidase that results in severe immunodeficiency. End-stage renal disease occurs in this patient population and is attributed to various factors, including infections, amyloidosis, and nephrotoxic anti-infective agents. In this report, we present our experience in transplantation for a patient with CGD complicated by isolated hepatic tuberculosis abscess. The course of the case demonstrates the absolute requirements for a multidisciplinary and compulsive approach before, during, and after transplantation. This case report also highlights the unexpectedly benign effects of immunosuppressive therapy in this patient population

Rapidly progressive subacute sclerosing panencephalitis presenting with acute loss of vision

A 10-year-old male presented with vision loss and behavioral changes. He had midpoint pupils with no reaction to light and normal funduscopic examination. Cranial magnetic resonance imaging revealed bilateral cortical lesions at parieto-occipital lobes. Elevated measles antibody titers in the cerebrospinal fluid confirmed the diagnosis of subacute sclerosing panencephalitis. Despite oral inosiplex and supportive care, patient developed generalized seizures with frequent myoclonic jerks and rapidly progressed into coma. Cortical blindness in subacute sclerosing panencephalitis can be an early indicator for fulminant course

Water epilepsy: A report of three cases

Water epilepsy is a rare form of the reflex epilepsies. In this paper, three patients who were followed up in our outpatient clinic is presented. Psychomotor development and neurological examination of patients were normal. The most important factor in the treatment approach is the temperature of the water. In treatment, avoiding bath with hot water or prophylaxis with benzodiazepines should be considered first. (Turk Arch Ped 2011; 46:259-60

Nephrocalcinosis as a complication of subcutaneous fat necrosis of the newborn

Subcutaneous fat necrosis of the newborn is an uncommon disorder affecting the adipose tissue of term infants. It is usually known as a transient, benign and self-limited disease, characterized by painful skin lesions beginning within the first week of life. The prognosis of the disease is generally good, but it may be complicated by potentially life-threatening metabolic alterations, including hypercalcemia, thrombocytopenia, hypoglycemia, and hypertriglyceridemia. Hypercalcemia is the most serious complication of subcutaneous fat necrosis because of its effects on the renal and cardiovascular systems. We thereby present a case of subcutaneous fat necrosis with all these metabolic alterations, which was also complicated by nephrocalcinosis as a non-transient and serious complication

Su ile ilişkili epilepsi: Üç olgu sunumu

Water epilepsy is a rare form of the reflex epilepsies. In this paper, three patients who were followed up in our outpatient clinic is presented. Psychomotor development and neurological examination of patients were normal. The most important factor in the treatment approach is the temperature of the water. In treatment, avoiding bath with hot water or prophylaxis with benzodiazepines should be considered first. (Turk Arch Ped 2011; 46:259-60