Moisture Damage in Ancient Masonry: A Multidisciplinary Approach for In Situ Diagnostics

San Nicola in Carcere, one of the minor basilicas in the historical center of Rome, was the location of a wide investigation campaign of the water-related deterioration causes, present in the lower sector of the apse and adjacent pillars, affected by water infiltrations, mould and salt efflorescence. The results obtained identify the presence of water content and related effects mainly on the sides of the apsidal wall. This work focuses on the use of five Non-Destructive Techniques (NDT) and intends to show the gains obtained by integrating widely interdisciplinary methods, namely the Infrared Thermography (IRT), the Unilateral Nuclear Magnetic Resonance (Unilateral NMR), the Acoustic Tomography (AT), the Acoustic Imaging (AI) and the Laser-Induced Fluorescence (LIF). All the techniques contribute to the rapid, non-invasive and early identification of the moisture distribution, while some of them (LIF and AI) also address the determination of some moisture effects. The integrated use of different techniques helps to take the multidisciplinary point of view necessary to formulate an effective restoration intervention based on a sound scientific rationale; nonetheless, it allows to experiment a holistic approach, verifying the potential of a wide range of NDTs available within the context of a restoration yard

PICUS: A Pocket-Sized System for Simple and Fast Non-Destructive Evaluation of the Detachments in Ancient Artifacts

An innovative, robust method has been developed, based on the use of a simple, compact, expressly designed device, named PICUS (the ‘woodpecker’ in ancient Latin), and inspired by the auscultation method carried out by the experts in the field of conservation of cultural heritage. This method entails gently knocking the surface, controlling and measuring the impact time of the stroke’s force, recording the generated sound, comparing the acquired sound with a reference sound by calculating the cross-correlation function, and its maximum, as a measure of the detachment. In a nutshell, it performs an analysis similar to that carried out by a professional who performs a routine examination on the detachments by hand. The experimental apparatus consists of a probe made of an electromechanical percussion element that gently taps the surface producing a sound, a force sensor purposely developed to measure the impact force, and a microphone, all connected with an Arduino-like low cost board, to record and elaborate the sounds and the force sensor signal. The probe XY position on the scene is recognized using an infra-red (IR) system with a low-cost IR camera and an IR light-emitting diode (IR-LED) positioned on the probe. The “tapper” and the microphone replace the hand and the ear of a conservator carrying out a detachment investigation, while the comparison with a reference is the typical mind process of a professional restorer. The result is the fusion of the microphone data and the force sensor data

Integration of Non-Destructive Acoustic Imaging Investigation with Photogrammetric and Morphological Analysis to Study the “<i>Graecia Vetus</i>” in the Chigi Palace of Ariccia

Integrating complementary information from many available technologies is a question of growing interest among the Cultural Heritage community, due to the complexity of the cultural assets under study and of their contexts. Recently, this need has pushed the development of appropriate data fusion procedures for this sector, among which the authors wish to propose their approach for treating multi-source data from image-based methodologies, experimented with in a representative case study. The Chigi Palace of Ariccia hosted our investigation campaign on a precious monochrome painting by Giuseppe Cades (1788), the Graecia Vetus. The study encompasses a photogrammetric survey and two acoustic diagnostic methods, the innovative Frequency Resolved Acoustic Imaging technique and the more traditional Acoustic Tomography. The photogrammetric survey allows reconstruction of the surface morphology of the painting, generating a 3D Digital Elevation Model, while the acoustic methods detect the structural damage beneath the surface due to detachments and flaws, generating 2D images. The output of this heterogeneous datasets fusion is a multi-layer map, each layer representing a type of dataset that clearly shows how some deformations of the surface morphology appear correlated with the presence of sub-surface anomalies, wide air cavities and more superficial detachments revealed by the acoustic diagnostic methods. Beside the exam of the conservation state of the Graecia Vetus, the proposed procedure effectively guarantees access to the integrated information, offering the possibility to understand the correlation between the causes and the effects of the decay process, as well as the retrieval of the single analysis in order to deepen one specific aspect

Moisture Damage in Ancient Masonry: A Multidisciplinary Approach for In Situ Diagnostics

San Nicola in Carcere, one of the minor basilicas in the historical center of Rome, was the location of a wide investigation campaign of the water-related deterioration causes, present in the lower sector of the apse and adjacent pillars, affected by water infiltrations, mould and salt efflorescence. The results obtained identify the presence of water content and related effects mainly on the sides of the apsidal wall. This work focuses on the use of five Non-Destructive Techniques (NDT) and intends to show the gains obtained by integrating widely interdisciplinary methods, namely the Infrared Thermography (IRT), the Unilateral Nuclear Magnetic Resonance (Unilateral NMR), the Acoustic Tomography (AT), the Acoustic Imaging (AI) and the Laser-Induced Fluorescence (LIF). All the techniques contribute to the rapid, non-invasive and early identification of the moisture distribution, while some of them (LIF and AI) also address the determination of some moisture effects. The integrated use of different techniques helps to take the multidisciplinary point of view necessary to formulate an effective restoration intervention based on a sound scientific rationale; nonetheless, it allows to experiment a holistic approach, verifying the potential of a wide range of NDTs available within the context of a restoration yard

Genomic characterization of undifferentiated sarcomatoid carcinoma of the pancreas

Undifferentiated sarcomatoid carcinoma (USC) of the pancreas is a rare but especially aggressive variant of pancreatic ductal adenocarcinoma (PDAC), composed of at least 80% of sarcomatoid cells. This study aimed to elucidate its clinicopathological and molecular features. The study cohort included 10 patients with pancreatic USC. Clinicopathological parameters were determined for each patient. The molecular profile was investigated using next-generation sequencing (NGS). Histologically, all tumors were hypercellular neoplasms with spindle-shaped or sarcomatoid cells. All patients showed vascular and perineural invasion. Most patients had a poor prognosis. NGS showed important similarities with conventional PDAC, including frequent alterations in the classic PDAC drivers, KRAS (100% of cases), TP53 (90%), and CDKN2A (60%). There were also some important distinctions from conventional PDAC: 1) SMAD4, a typical PDAC driver gene, was mutated in only one case (10%); 2) Another distinctive molecular feature was the recurrent KRAS amplification (30% of cases), which is very rare in conventional PDAC. It has been previously reported in another subtype of pancreatic undifferentiated carcinoma, the rhabdoid variant, and may be a key event leading to the acquisition of an undifferentiated phenotype in a subgroup of cases; 3) Lastly, in two different cases, we detected two potentially actionable targets, not belonging to the typical PDAC molecular landscape, such as MCL1 amplification and POLQ mutation. Our study sheds light on this rare tumor type, which shows aggressive biological behavior and few druggable alterations. The most distinctive molecular features of pancreatic USC are the paucity of SMAD4 alterations and recurrent KRAS amplification