Antimicrobial resistance gene shuffling and a three-element mobilisation system in the monophasic Salmonella typhimurium strain ST1030

In this study we describe the genetic elements and the antimicrobial resistance units (RUs) harboured by the Salmonella Typhimurium monophasic variant 1,4,[5],12:i:- strain ST1030. Of the three identified RUs two were chromosomal, RU1 (IS26-blaTEM-1-IS26-strAB-sul2- IS26) and RU2 (IS26-tetR(B)-tetA(B)-ΔIS26), and one, RU3 (a sul3-associated class 1 integron with cassette array dfrA12-orfF-aadA2-cmlA1-aadA1), was embedded in a Tn21-derived element harboured by the conjugative I1 plasmid pST1030-1A. IS26 elements mediated the antimicrobial resistance gene (ARG) shuffling and this gave rise to pST1030-1A derivatives with different sets of ARGs. ST1030 also harboured two ColE1-like plasmids of which one, pST1030-2A, was mobilisable and the target of an intracellular translocation of the Tn21-derived element; the second (pST1030-3) was an orphan mob-associated oriT plasmid co-transferred with pST1030-1A and pST1030-2A. pST1030-2A and pST1030-3 also carried a parA gene and a type III restriction modification system, respectively. Overall analysis of our data reinforces the role played by IS26, Tn21-derived elements and non-conjugative plasmids in the spread of ARGs and supplies the first evidence, at least in Salmonella, for the identification of a natural isolate harbouring a three-element mobilisation system in the same cell

Diabetes insipidus secondary to nivolumab-induced neurohypophysitis and pituitary metastasis

A 62-year-old patient with metastatic hypopharyngeal carcinoma underwent treatment with nivolumab, following which he developed symptoms suggestive of diabetes insipidus. Nivolumab was stopped and therapy with methylprednisolone was started. During corticosteroid therapy, the patient presented himself in poor health condition with fungal infection and glycemic decompensation. Methylprednisolone dose was tapered off, leading to the resolution of mycosis and the restoration of glycemic compensation, nevertheless polyuria and polydipsia persisted. Increase in urine osmolarity after desmopressin administration was made diagnosing central diabetes insipidus as a possibility. The neuroradiological data by pituitary MRI scan with gadolinium was compatible with coexistence of metastatic localization and infundibuloneurohypophysitis secondary to therapy with nivolumab. To define the exact etiology of the pituitary pathology, histological confirmation would have been necessary; however, unfortunately, it was not possible. In the absence of histological confirmation, we believe it is likely that both pathologies coexisted

Optimization of cw sodium laser guide star efficiency

Context: Sodium laser guide stars (LGS) are about to enter a new range of laser powers. Previous theoretical and numerical methods are inadequate for accurate computations of the return flux and hence for the design of the next-generation LGS systems. Aims: We numerically optimize the cw (continuous wave) laser format, in particular the light polarization and spectrum. Methods: Using Bloch equations, we simulate the mesospheric sodium atoms, including Doppler broadening, saturation, collisional relaxation, Larmor precession, and recoil, taking into account all 24 sodium hyperfine states and on the order of 100 velocity groups. Results: LGS return flux is limited by "three evils": Larmor precession due to the geomagnetic field, atomic recoil due to radiation pressure, and transition saturation. We study their impacts and show that the return flux can be boosted by repumping (simultaneous excitation of the sodium D2a and D2b lines with 10-20% of the laser power in the latter). Conclusions: We strongly recommend the use of circularly polarized lasers and repumping. As a rule of thumb, the bandwidth of laser radiation in MHz (at each line) should approximately equal the launched laser power in Watts divided by six, assuming a diffraction-limited spot size.Comment: 15 pages, 12 figures, to be published in Astronomy & Astrophysics, AA/2009/1310

Prevalence and risk factors for chronic obstructive lung disease in HIV-infected patients in the HAART era

Our results suggest a role for HIV infection itself and for current cigarette smoking in the development of respiratory symptoms and COPD in HIV-infected patients. HAART did not seem to reduce the risk of respiratory symptoms and COPD, in our cases. Thus, our results suggest that HIV-infected patients should be screened for chronic respiratory disease in order to early identify those at risk or those who need specific treatment

Geomonumental routes: the granitic bridges over the Guadarrama river (Madrid, Spain) and the calcarenitic coastal towers from the Salento (Italy)

8 pages, 7 figures.-- Published in: Proceedings of the 11th International Congress on Deterioration and Conservation of Stone (Torun Poland, 15-20 September 2008), eds. Jadwiga W. Lukaszewicz and Piotr Niemcewicz.-- Presentation in PDF format available at: https://digital.csic.es/handle/10261/7648.This paper focuses on the new concept of GeoMonumental Routes, which mainly consists of the dissemination of architectural heritage with the added value of geology. Geology, so far, has not been considered in all its aspects in architectural heritage: i.e. geography, geomorphology, quarries provenance, building stones, and their relationship with historical and architectural aspects, constructive techniques and technological developments, as well as the connection of heritage structures to the settlement of historical routes. For this purpose, two scientific teams have gathered to develop two of this kind of routes following a common methodology, based on different geographical context, geological settlement, history, structure tipology and building stones.Thanks are given to both Ministries of Education from Spain and Italy for granting the CSIC-CNR bilateral cooperation project (2006IT0021).Peer reviewe