All‐In‐One OsciDrop Digital PCR System for Automated and Highly Multiplexed Molecular Diagnostics

Digital PCR (dPCR) holds immense potential for precisely detecting nucleic acid markers essential for personalized medicine. However, its broader application is hindered by high consumable costs, complex procedures, and restricted multiplexing capabilities. To address these challenges, an all‐in‐one dPCR system is introduced that eliminates the need for microfabricated chips, offering fully automated operations and enhanced multiplexing capabilities. Using this innovative oscillation‐induced droplet generation technique, OsciDrop, this system supports a comprehensive dPCR workflow, including precise liquid handling, pipette‐based droplet printing, in situ thermocycling, multicolor fluorescence imaging, and machine learning‐driven analysis. The system's reliability is demonstrated by quantifying reference materials and evaluating HER2 copy number variation in breast cancer. Its multiplexing capability is showcased with a quadruplex dPCR assay that detects key EGFR mutations, including 19Del, L858R, and T790M in lung cancer. Moreover, the digital stepwise melting analysis (dSMA) technique is introduced, enabling high‐multiplex profiling of seven major EGFR variants spanning 35 subtypes. This innovative dPCR system presents a cost‐effective and versatile alternative, overcoming existing limitations and paving the way for transformative advances in precision diagnostics

A Discussion on Adaptive Applications of Some Chinese Design Codes in Guinea and Localization of Flood Standards

Chinese engineering team participated in the construction of several water conservancy and hydropower projects in areas which have differences in climate characteristics from China. Due to a lack of hydrological and climatic data and the absence of systematic design specifications in these areas, considering construction safety, most of the Chinese designs and constructions are based on the extreme values with the Chinese design codes. The S Project in Guinea established a rainfall-runoff model through open-source remote sensing satellite precipitation data and limited hydrological station measured runoff data，allowing to refine local hydrological data. Based on these data series, it was then possible to calculate flood control standards with more local hydrological characteristics in order to promote the progress of the project scientifically. In addition, combining the refined discharging curve of the outlet hole with the latest arc gate online monitoring technology, and the joint dispatch with the downstream hydropower station, permitted that the downstream K Hydropower Station created historic power generation in 2019 with significant economic and social benefits

Research on User Identity Authentication Based on Two-way Confirmation in Data Transmission

User identity authentication is the foundation of data transmission in the complicated network environment. Moreover, the key issue is the effective identity authentication of both sides in data transmission. An authentication method for user identity based on two-way confirmation in data transmission is proposed in this paper. The public key, private key, information of traditional identity authentication, one-time transmission key, timestamp, authentication lifecycle for timestamp and other authentication elements are constructed. Based on guaranteeing the timeliness of data transmission, the two-way user identity authentication process for sending terminal and receiving terminal is set up through using the information of traditional identity authentication and one-time transmission key

OsciDrop: A Versatile Deterministic Droplet Generator

This paper describes OsciDrop, a versatile chip-free droplet generator used to produce size-tunable droplets on demand. Droplet generation is fundamental to miniaturized analysis. We designed OsciDrop to segment the fluid flowing out of the orifice of a disposable pipette tip into droplets by oscillating its distal end underneath an immiscible continuous phase. We described the theoretical model and investigated the effect of flow rate, oscillating amplitude, frequency, and waveform on droplet generation. Our study revealed a previously underexplored Weber number-dominated regime that leverages inertial force instead of viscous force to generate droplets. The same pipette tip allowed robust and deterministic generation of monodisperse droplets with programmable sizes ranging from 200 pL to 2 mu L by asymmetrical oscillation. We validated this platform with two droplet-based nucleic acid amplification tests: a digital loop-mediated isothermal amplification assay for absolute quantification of African swine fever virus and a multi-volume digital polymerase chain reaction assay for the high dynamic range measurement of human genomic DNA. The OsciDrop method opens a facile avenue to miniaturization, integration, and automation, exhibiting full accessibility for digital molecular diagnostics