Binding Assay of Cytosolic Proteins to the Cytoskeleton

n/

Environmental Stress and Plants 2.0

Following the success of our previous edition [1], this Special Issue ‘Environmental Stress and Plants 2.0’ includes 23 original articles and 3 reviews. The high level of participation and the large number of reports show that plant researchers are interested in this topic. We provide a brief overview of the papers, which include original articles (categorized by stress type) as well as reviews

Pollen-Pistil Interaction

The aim of this Special Issue is to highlight the molecular dialogue between the pollen tube and the pistil. This is achieved with original articles and reviews which show how this dialogue is controlled at the genomic and molecular levels. During the angiosperm’s double fertilization, the pollen tube must enter female tissues, bypass numerous physical barriers to reach the micropyle, and release gametes to complete the fertilization process with the final fusion between male and female gametes. There are molecular signals produced by the pistil that are intercepted by the receptors located primarily at the tip of the tube, which generate effects that modulate its growth activity; in turn, the pollen tube releases molecules that determine effects on the pistil cells. Thus, a complex dialogue develops between the female and male counterparts, whose language is made up of an expansive molecular alphabet that includes proteins, glycoproteins, arabinogalactan-proteins, lipid-binding proteins, nanovesicles, ions, amino acids, sugars, hormones, reactive oxygen species (ROS), and modulators of gene expression

Pollen-Pistil Interaction

The aim of this Special Issue is to highlight the molecular dialogue between the pollen tube and the pistil. This is achieved with original articles and reviews which show how this dialogue is controlled at the genomic and molecular levels. During the angiosperm’s double fertilization, the pollen tube must enter female tissues, bypass numerous physical barriers to reach the micropyle, and release gametes to complete the fertilization process with the final fusion between male and female gametes. There are molecular signals produced by the pistil that are intercepted by the receptors located primarily at the tip of the tube, which generate effects that modulate its growth activity; in turn, the pollen tube releases molecules that determine effects on the pistil cells. Thus, a complex dialogue develops between the female and male counterparts, whose language is made up of an expansive molecular alphabet that includes proteins, glycoproteins, arabinogalactan-proteins, lipid-binding proteins, nanovesicles, ions, amino acids, sugars, hormones, reactive oxygen species (ROS), and modulators of gene expression

Plant Fibers and Phenolics: A Review on Their Synthesis, Analysis and Combined Use for Biomaterials with New Properties

Devising environmental-friendly processes in biotechnology is a priority in the current economic scenario. We are witnessing a constant and steady push towards finding sustainable solutions to societal challenges by promoting innovation-driven activities minimizing the environmental impact and valorizing natural resources. In bioeconomy, plants are among the most important renewable sources of both fibers (woody and cellulosic) and phytochemicals, which find applications in many industrial sectors, spanning from the textile, to the biocomposite, medical, nutraceutical, and pharma sectors. Given the key role of plants as natural sources of (macro)molecules, we here provide a compendium on the use of plant fibers functionalized/impregnated with phytochemicals (in particular phenolic extracts). The goal is to review the various applications of natural fibers functionalized with plant phenolics and to valorize those plants that are source of both fibers and phytochemicals

Pasta Enriched with Carrot and Olive Leaf Flour Retains High Levels of Accessible Bioactives after In Vitro Digestion

The aim of this research was to evaluate the levels of antioxidants and polyphenols in pasta enriched with either carrot or olive leaf flours after simulating gastrointestinal digestion. Pasta samples were prepared with fixed amounts of carrot and olive leaf flours (15% and 6% of the total mixture, respectively). We measured the antioxidant capacity and polyphenol content at different stages of the pasta production process, starting from the initial flour to the cooked pasta, and tested samplesoftheliquid componentandsolidwasteresultingfromthedigestionprocess. Theantioxidant activity was measured by the FRAP method, while the polyphenol content was measured by the Folin–Ciocalteu method. Vitamin E contents were measured by HPLC. The pasta enriched with carrot (1.26 0.05 mmol/100 g) and olive leaf (2.9 0.07 mmol/100 g) exhibited higher antioxidant power compared to the unenriched pasta (0.8 0.1 mmol/100 g). The polyphenol content followed a similar trend, with values of 131.23 3.08 for olive flour-enriched pasta, 79.15 1.11 for carrot flour-enriched pasta, and 67.5 1.39 for the wheat-only pasta. The pasta samples maintained their antioxidant and polyphenol levels even after undergoing the simulated digestion process. Significantly, the liquid component of the pasta with olive leaf flours had the highest levels of antioxidants and polyphenols during all stages of the digestion process. According to the results of this study, pasta enriched with carrot and olive leaf flours shows promising potential for improving nutritional and functional properties by increasing antioxidant and polyphenol content. The samples were also evaluated by a sensory panel, which showed that fortification modified the perception of some organoleptic attributes without affecting the overall taste of the pasta

Drought Stress Affects the Response of Italian Local Tomato (Solanum lycopersicum L.) Varieties in a Genotype-Dependent Manner

Drought stress is one of the most severe conditions for plants, especially in the face of the emerging problem of global warming. This issue is important when considering economically relevant crops, including the tomato. For these plants, a promising solution is the valorization of local agrobiodiversity as a source of genetic variability. In this paper we investigated how six Italian tomato varieties react to a prolonged period of water depletion. We used a multidisciplinary approach, from genetics to plant physiology and cytology, to provide a detailed overview of the response of plants to stress. The varieties analyzed, each characterized by a specific genetic profile, showed a genotype-specific response with the variety 'Fragola' being the most resistant and the variety 'Pisanello' the most susceptible. For all the parameters evaluated, 'Fragola' performed in a manner comparable to that of control plants. On the contrary, 'Pisanello' appeared to be more affected and showed an increase in the number of stomata and a drastic increase in antioxidants, a symptom of acute oxidative stress. Our work suggests the existence of a valuable reservoir of genetic biodiversity with more drought-tolerant tomato genotypes opening the way to further exploitation and use of local germplasm in breeding programs

Effects of UV-B Radiation on the Performance, Antioxidant Response and Protective Compounds of Hazelnut Pollen

Increasing ultraviolet (UV) radiation is expected to become a problem in hazelnut cultivation. The aim of this study is to examine the effects of UV-B on hazelnut pollen. To this end, the pollens were exposed to UV-B for 1, 2, and 3 h at distances of 10, 20, 30, and 40 cm. Groups treated for 2 h at 20 cm and 3 h at 10 and 20 cm were identified as the most affected based on the results of viability, germination, and tube elongation. Further studies on these groups showed that UV-B does not change the DPPH radical scavenging activity for all groups. However, total phenolic compounds decreased after 3 h of treatment at 10 and 20 cm, while total flavonoid compounds decreased after all treatment groups. The UV-B absorbance of cytoplasmic and cell-wall-bound fractions decreased for all groups. The UV-B absorbance of the sporopollenin-derived fraction increased after 2 h of treatment at 20 cm but decreases after treatment for 3 h at 10 and 20 cm. In summary, exposure to UV-B for different times and distances adversely affected pollen grains in terms of pollen viability, germination rate, tube length, and the level of antioxidant molecules and UV-absorbing compounds

Editorial: Regulation of pollen tube growth, volume II

The pollen tube is an extension produced by the pollen grain when conditions are favorable; thus, the pollen tube is important in seed plant reproduction because it transports male gametes. However, it is also an excellent system for studying various plant cell processes that are common to sink organs or tissues (Kroeger and Geitmann, 2012). The pollen tube has been used to study a variety of processes, including vesicular transport, cytoskeletal organization, cell wall deposition, ion gradients, intracellular signaling. Since the pollen tube grows by contacting and signaling to pistil cells, it is also a model for studying cell-cell communication (Broz and Bedinger, 2021). Moreover, the pollen tube is involved in self-incompatibility (SI) processes that regulate reproduction and thus promote hybridization and genetic variability (Mandrone et al., 2019). SI is regulated by several factors, and in some cases, such as citrus, it is an important tool for producing seedless mandarins (Gentile et al., 2012). Pollen tube and pollen can also be targets of environmental stresses (Ledesma and Sugiyama, 2019), which can impair plant reproductive success, resulting in lower productivity of agronomically important plants and increasing allergenicity (pollinosis) (Armentia et al., 2019; Singh and Mathur, 2021)