Long-term Acid Deposition Effects on Soil and Water Chemistry in the Noland Divide Watershed, Great Smoky Mountains National Park, USA

Impacts of long-term acid deposition on soil and water chemistry are of particular concern in the Great Smoky Mountains National Park (GRSM), receiving some of the highest acid deposition rates in the eastern United States and limited by inadequate acid buffering capacity. In the GRSM, the Noland Divide watershed (NDW) has been monitored since 1991 for water chemistries of precipitation, throughfall, soil, and stream. The impacts of long-term acidic deposition on stream water quality in the NDW were investigated through: 1) development of an ion input-output budget; 2) analysis of trends and seasonal patterns for major ions; 3) comparison of net export rates between baseflow and stormflow periods; and 4) characterization of soil chemistry and transport properties for various potential acid deposition scenarios. Between 1991 and 2006, throughfall deposition remained unchanged and consisted of 1,735 eq ha-1 yr-1 of SO42-, 863 eq ha-1 yr-1 of NO3-, and 284 eq ha-1 yr-1 of NH4+. Net retention of sulfate was estimated at 61% being controlled by soil adsorption, and inorganic nitrogen was retained at 32% presumably due to plant uptake. Nitrogen retention increased by 44.30 eq ha-1 yr-1 over the study period. Besides deposition, soil acidity was increased by nitrification and precipitation-driven desorption of previously accumulated sulfate. To neutralize soil acidity, Ca2+, Mg2+ and Na+ were depleted from NDW by 77, 46 and 66 eq ha-1 yr-1, respectively. Due to the continuous addition of acidity, base saturation in soil was reduced to 4% at present. Mobilization of aluminum and other metals (Mn and Zn) may be enhanced by these geochemical processes, potentially causing toxic conditions to fish and other biota in the GRSM streams. Impacts of acidic deposition on streams were confirmed by measured stream pH below 6 and acid neutralizing capacity below 0.01 meq L-1. During stormflows pH and ANC depressions occurred primarily due to increases in sulfate transport, leading to episodic acidification events. This study provides new information on hydrological and biogeochemical processes that regulate stream acidification events in the southern Appalachian region, supporting improved management strategies for GRSM streams

Natural Resources Research Institute Technical Report

Water temperature is generally considered one of the primary physical habitat parameter determining the suitability of stream habitat for fish species, with effects on the mortality, metabolism, growth, behavior, and reproduction of individuals. In this study we assessed the potential threats of climate change on stream temperatures and flow regimes in Lake Superior tributary streams in Minnesota, USA. The study included deterministic models for stream flow and temperature of three study streams (Amity Creek, Baptism River, Knife River), and regional (empirical) models for specific flow and temperature parameters to give better spatial coverage of the region. Information on stream flow, stream temperature, and land cover was used to develop a brook trout presence/absence model to understand the current pattern of distribution of brook trout and predict future distributions under future climate. The hydrology of north shore streams is mainly driven by air temperature and precipitation. Historical air temperatures in the region have a significant upward trend, particularly since 1980. Global climate model (GCM) outputs project a continued increasing trend in air temperature, with an increase in mean annual air temperature of 2 to 3 °C by 2089. The historical precipitation data shows an increasing trend for total annual precipitation at Duluth and Two Harbors between 1900 and 2010, whereas Grand Marais and Grand Portage do not have a clear trend. Based on an analysis of daily precipitation totals, there is some indication of an increasing trend in the number of days in summer with high precipitation (10-20 cm). Both the GENMOM and the ECHAM5 GCMs project overall increases in precipitation of about 15%, but differ with respect to the seasonal distribution of the precipitation changes. A significant and relatively certain impact of climate change is a projected shift in precipitation from snowfall to rainfall. While an increasing trend in precipitation leads to increasing streamflow, the increasing trend in spring and summer air temperature tends to reduce streamflow (by increasing evapotranspiration). Available streamflow records for north shore streams suggest there may be a decreasing trend in mean annual flow and summer low flow, but the trends are not statistically significant. Future projections of streamflow based on the GCM output were mixed, with the deterministic models projecting moderate increases in average stream flow and summer low flow, while the regression models for project a moderate decrease in low flow. Stream temperature analyses for the three study streams based on GCM climate output give the result of fairly uniform seasonal increases in stream temperature to 2089 ranging from 1.3 to 1.9 °C for the GENMOM model to 2.2 to 3.5°C for the ECHAM5 model. Application of the GENMOM climate data to the deterministic stream temperature models produced fairly similar stream temperature changes for the three study sites. The empirical stream temperature study found stream temperature in the north shore region to be influenced by air temperature, catchment size, percentage of woody wetlands, latitude, and soil permeability rate. In response to climate change projected by the GENMOM GCM, the regional stream temperature model projects July mean water temperature to rapidly increase by approximately 1.2oC from 1990s to 2060s, followed by a slight decrease to 2089. The temperature increase was predicted to be the largest in the coastal area of middle north shore region. The brook trout presence/absence model found water temperature to have the strongest influence on trout presence. Brook trout were predicted to be at risk for water temperatures above 18.7oC and be extirpated from streams for temperatures over 20oC. Stream flow was shown to have a negative effect on trout presence, though not as strong as water temperature. Overall, these data predict that brook trout may be extirpated from lower shore area, be exposed to increasing risk in middle shore region, and remain present in upper shore streams from the present to 2089. This work would benefit greatly from a number of modifications to the GCM’s, the spatial data used in the development of both the deterministic and empirical models, and implementation of a more detailed, spatially explicit, hydrologic model. Finally, additional fish data, including cool and warm water assemblage data, along with descriptors of landscape structure (i.e., connectivity) would allow us to assess the areas where cold water species may be threatened by the presence or potential presence of coolwater competitors

Comparing Properties of Water Absorbing/Filtering Media for Bioslope/Bioswale Design

Drainage from highways, particularly the first flush of runoff, contains high levels of contaminants such as suspended solids, metals, and organics. To restrict the discharge of polluted stormwater, the National Pollutant Discharge Elimination System (NPDES) State Disposal System (SDS) General Permit issued by Minnesota Pollution Control Agency (MPCA) in 2013 requires that the first inch of stormwater runoff from new impervious should be held on site through infiltration, harvesting or reuse. Multiple types of infiltration materials have been studied in the laboratory and the field, but few studies have considered the application of local materials for best management practices (BMP). The objective of this project is to determine the characteristics of various naturally occurring water adsorbing and filtering media, such as peat and muck, found along road construction projects in northern Minnesota. Salvage and reuse of these materials during road construction will be evaluated for stormwater treatment, including absorption, infiltration, filtration, and pollutant capture, in constructed vegetated slopes along highway right of ways. The naturally occurring material will be compared to leaf and grass feedstock compost

Development and Regionalization of In Situ Bioslopes and Bioswales

This project is a multi-disciplinary investigation into the use of alternative media for biofiltration systems in Minnesota. Over the last thirty years, the Minnesota Department of Transportation (MnDOT) has implemented biofilters along roadways as a stormwater control measure. These systems must be able to infiltrate and treat the first inch of rainfall onsite to meet state and national regulations. The performance of a biofilter is largely based on its media?s ability to infiltrate water, sustain vegetation, and capture pollutants. To date, MnDOT has relied on sand and compost mixtures for biofilter media components. An early phase of this work identified peat as having similar performance characteristics as compost, making it an ideal alternative. A laboratory testing program was also developed during the early phase of work to determine media properties that could be used to predict biofilter performance. This project focused on characterizing existing biofilters using in situ testing and comparing results to laboratory testing. The comparison of the two methods demonstrated the predictive capabilities of the laboratory regime. This project also included the instrumentation and monitoring of field sites including a newly constructed peat amended biofilter. Findings from this work determined the validity of using peat for future biofilters and can aid in identifying and characterizing other alternative media

Historical watershed stressors for the Laurentian Great Lakes, links to resources and data

These data are a detailed set of historical stressor data for 60 watersheds comprising the Laurentian Great Lakes basin. Archival records were transcribed to create quantitative data on human activities: population, mining, deforestation and agriculture. Yearly records of stressors are provided from 1780 through 2010

Remote control of the recruitment and capture of endogenous stem cells by ultrasound for in situ repair of bone defects

Stem cell-based tissue engineering has provided a promising platform for repairing of bone defects. However, the use of exogenous bone marrow mesenchymal stem cells (BMSCs) still faces many challenges such as limited sources and potential risks. It is important to develop new approach to effectively recruit endogenous BMSCs and capture them for in situ bone regeneration. Here, we designed an acoustically responsive scaffold (ARS) and embedded it into SDF-1/BMP-2 loaded hydrogel to obtain biomimetic hydrogel scaffold complexes (BSC). The SDF-1/BMP-2 cytokines can be released on demand from the BSC implanted into the defected bone via pulsed ultrasound (p-US) irradiation at optimized acoustic parameters, recruiting the endogenous BMSCs to the bone defected or BSC site. Accompanied by the daily p-US irradiation for 14 days, the alginate hydrogel was degraded, resulting in the exposure of ARS to these recruited host stem cells. Then another set of sinusoidal continuous wave ultrasound (s-US) irradiation was applied to excite the ARS intrinsic resonance, forming highly localized acoustic field around its surface and generating enhanced acoustic trapping force, by which these recruited endogenous stem cells would be captured on the scaffold, greatly promoting them to adhesively grow for in situ bone tissue regeneration. Our study provides a novel and effective strategy for in situ bone defect repairing through acoustically manipulating endogenous BMSCs

The delayed clearance of Talaromyces marneffei in blood culture may be associated with higher MIC of voriconazole after antifungal therapy among AIDS patients with talaromycosis.

ObjectivesThis study aimed to investigate the influencing factors of delayed clearance of Talaromyces marneffei (T. marneffei) in blood culture of patients with acquired immune deficiency syndrome (AIDS) complicated with talaromycosis after antifungal therapy.MethodsThe patients with AIDS complicated with talaromycosis were retrospectively enrolled, and divided into two groups according to the blood T. marneffei culture results in two weeks after antifungal therapy. The baseline clinical data were collected and the antifungal susceptibility of T. marneffei was tested.ResultsA total of 190 patients with AIDS and talaromycosis were enrolled, of whom 101 cases remained positive for T. marneffei (Pos-group) while the other 89 cases were negative in blood culture (Neg-group) after two weeks' antifungal treatment. The Pos-group had a higher baseline Aspartate aminotransferase (AST, 78.5 vs. 105 U/L; P = 0.073) and lower CD4+ T cells level (11 vs. 7 cells/μl; P = 0.061). The percentage of isolates with higher MICs of voriconazole (VOR) and fluconazole (FLU) in the Pos-group were significantly higher than those in the Neg-group (χ2 = 12.623, P ConclusionsThe delayed negative conversion of blood T. marneffei-culture may be associated with some factors especially higher MIC of VOR, indicating the possibility of drug resistance of T. marneffei

Natural Resources Research Institute Technical Report

The impact of improved genetics from the University of Minnesota Duluth Natural Resources Research Institute (NRRI) hybrid poplar breeding program on breakeven hybrid poplar wood prices is discussed in this paper. After a review of previous economic analyses, the breakeven prices are presented that would cover costs other than land rent. Then, factors are provided for adjusting the price to reflect the land rental rate for a given location. The breakeven prices are presented both as stumpage and delivered to a mill. Breakeven delivered prices include the stumpage values plus conventional harvest and transportation costs to a hypothetical processing plant. Breakeven land rental rates are also provided at which poplar production would be profitable at recent aspen stumpage prices. The impact of improved genetics from the University of Minnesota Duluth Natural Resources Research Institute (NRRI) hybrid poplar breeding program on breakeven hybrid poplar wood prices is discussed in this paper. After a review of previous economic analyses, the breakeven prices are presented that would cover costs other than land rent. Then, factors are provided for adjusting the price to reflect the land rental rate for a given location. The breakeven prices are presented both as stumpage and delivered to a mill. Breakeven delivered prices include the stumpage values plus conventional harvest and transportation costs to a hypothetical processing plant. Breakeven land rental rates are also provided at which poplar production would be profitable at recent aspen stumpage prices. The analysis is based on a scenario where a biorefinery/bioproducts company owns and/or leases the land, controls the harvest and transportation of the wood, and delivers the wood to their own mill. The evaluation is based on two hybrid poplar annual growth increments: 3.6 dry tons and 5.4 dry tons per acre per year with a 9-, 10-, or 12-year rotation. 3.6 dry tons per acre per year is yield potential with current genotypes. 5.4 tons per acre per year is yield potential with new Gen 1.0 elite clones from our breeding. Stumpage prices without land cost included are lower than aspen stumpage prices for both unimproved and improved clones. Other things being equal, the improved genetics could be capitalized into a $36.37-per-acre increase in the financially permissible rental rate. Carbon credit markets could further improve returns on hybrid poplar plantations.USDA-NIFA Agriculture and Food Research Initiative Competitive Grants Program Sustainable Bioenergy and Bioproducts Challenge Area (Grant No. 2018-68005-27635/Project Accession No. 1015244