Prevalence of pSCFS7-like vectors among cfr-positive staphylococcal population in Spain

Sin financiación4.615 JCR (2018) Q1, 15/89 Infectious Diseases, 29/133 Microbiology, 32/267 Pharmacology & Pharmacy1.531 SJR (2018) Q1, 55/298 Infectious Diseases, 22/127 Microbiology (medical), 30/268 Pharmacology (medical), 251/2844 Medicine (miscellaneous)No data IDR 2018UE

Adult hippocampal neurogenesis is abundant in neurologically healthy subjects and drops sharply in patients with Alzheimer's disease

The hippocampus is one of the most affected areas in Alzheimer's disease (AD)1. Moreover, this structure hosts one of the most unique phenomena of the adult mammalian brain, namely, the addition of new neurons throughout life2. This process, called adult hippocampal neurogenesis (AHN), confers an unparalleled degree of plasticity to the entire hippocampal circuitry3,4. Nonetheless, direct evidence of AHN in humans has remained elusive. Thus, determining whether new neurons are continuously incorporated into the human dentate gyrus (DG) during physiological and pathological aging is a crucial question with outstanding therapeutic potential. By combining human brain samples obtained under tightly controlled conditions and state-of-the-art tissue processing methods, we identified thousands of immature neurons in the DG of neurologically healthy human subjects up to the ninth decade of life. These neurons exhibited variable degrees of maturation along differentiation stages of AHN. In sharp contrast, the number and maturation of these neurons progressively declined as AD advanced. These results demonstrate the persistence of AHN during both physiological and pathological aging in humans and provide evidence for impaired neurogenesis as a potentially relevant mechanism underlying memory deficits in AD that might be amenable to novel therapeutic strategies.Plan Nacional I+D.36.130 JCR (2019) Q1, 3/195 Cell Biology, 2/297 Biochemistry & Molecular Biology, 1/139 Medicine, Research & Experimental15.812 SJR (2019) Q1, 2/271 Biochemistry, Genetics and Molecular Biology (miscellaneous), 5/2754 Medicine (miscellaneous)No data IDR 2019UE

The emergence of plasmid-borne cfr-mediated linezolid resistant-staphylococci in Vietnam

Objectives Linezolid is one of the last resort antibiotics effectively used in the treatment of infections caused by multidrug-resistant Gram-positive bacteria. Recent outbreaks of Linezolid resistance have been the great concern worldwide, while many countries have not experienced it. In this work, we aimed to evaluate the existence of linezolid resistance and further clarify potential resistance mechanism(s) in staphylococcal isolates obtained from the hospital in Vietnam, a country in which linezolid resistance had not been previously detected. Methods Seventy staphylococcal clinical isolates including MRSA (n = 63) and methicillin-resistant coagulase-negative staphylococci (MRCNS, n = 7) were collected and analyzed for linezolid resistance. Linezolid-resistant isolates were submitted for whole genome sequencing to search for the resistance determinants. Results We identified two coagulase-negative staphylococcal isolates that were resistant to linezolid. Whole genome sequencing revealed several alterations in the 23S rRNA and L3, L17, L22, L24, L30 ribosomal proteins. Importantly, both isolates harbour the chloramphenicol/florfenicol resistance (cfr) gene on a plasmid. The plasmid was closely identical to the pLRSA417 plasmid that was originally reported in China. Conclusions To the best of our knowledge, this is the first report of cfr-mediated linezolid resistance in clinically isolated staphylococci in Vietnam. We suggest that adequate surveillance is necessary to monitor the dissemination of linezolid resistance among staphylococcal species and other important pathogens.Sin financiación4.035 JCR (2020) Q2, 34/93 Infectious Diseases0.917 SJR (2020) Q2, 98/197 Immunology and AllergyNo data IDR 2020UE