Production and carcass traits of high dairy genetic merit Holstein, standard dairy genetic merit Friesian and Charolais × Holstein-Friesian male cattle

peer-reviewedThe increased proportion of Holstein genetic material in the dairy herd has consequences for beef production in Ireland. A total of 72 spring-born male calves (24 Holsteins (HO), 24 Friesian (FR) and 24 Charolais × Holstein-Friesians (CH)) were reared from calfhood to slaughter. Calves were artificially reared indoors and spent their first summer at pasture following which they were assigned, on a breed basis, to a factorial combination of two production systems (intensive 19-month bull beef and extensive 25-month steer beef) and two slaughter weights (560 and 650 kg). After slaughter the pistola hind quarter was separated into fat, bone and muscle. Live-weight gain, carcass gain, kill-out proportion, carcass conformation and carcass fat scores were 830, 811 and 859 (s.e. 14.9) g/day, 540, 533, 585 (s.e. 7.7) g/day, 526, 538 and 561 (s.e. 3.0) g/kg, 1.51, 2.18 and 2.96 (s.e. 0.085), and 3.40, 4.25 and 4.06 (s.e. 0.104) for HO, FR and CH, respectively. Corresponding values for pistola weight as a proportion of carcass weight, pistola muscle proportion and pistola fat proportion were 458, 459 and 461 (s.e. 2.6) g/kg, 657, 645 and 667 (s.e. 3.7) g/kg, and 132, 161 and 145 (s.e. 4.1) g/kg. Compared with the intensive system, animals on the extensive system had a lower (P < 0.001) daily live-weight gain, kill-out proportion and a lower muscle proportion in the pistola. Increasing slaughter weight increased (P < 0.001) carcass weight and carcass fat score and reduced the proportion of muscle in the pistola. Allometric regression coefficients for pistola weight on side weight, and total bone, muscle and fat weights on pistola weight were 0.898, 0.755, 0.900 and 1.910 respectively. It is concluded that HO grew at least as fast as FR but had a lower killout proportion. Carcass conformation and fat scores were greater for FR than for HO and muscle proportion in the pistola was lower and total fat proportion was higher. Compared with FR, CH had heavier carcasses, a higher kill-out proportion and less fat and more muscle in the pistola

Body and carcass measurements, carcass conformation and tissue distribution of high dairy genetic merit Holstein, standard dairy genetic merit Friesian and Charolais x Holstein-Friesian male cattle

peer-reviewedThe increased proportion of Holstein genes in the dairy herd may have undesirable consequences for beef production in Ireland. A total of 72 spring-born calves, (24 Holstein (HO), 24 Friesian (FR) and 24 Charolais X Holstein-Friesian (CH)) were reared from calfhood to slaughter. Calves were artificially reared indoors and spent their first summer at pasture following which they were assigned to a 3 breeds (HO, FR and CH) 2 production systems (intensive 19-month bull beef and extensive 25-month steer beef) 2 slaughter weights (560 and 650 kg) factorial experiment. Body measurements of all animals were recorded at the same time before the earliest slaughter date. After slaughter, carcasses were graded and measured and the pistola hind-quarter was separated into fat, bone and muscle. HO had significantly higher values for withers height, pelvic height and chest depth than FR, which in turn had higher values than CH. HO had a longer back and a narrower chest than either FR or CH, which were not significantly different. Carcass length and depth, pistola length, and leg length were 139.2, 134.4 and 132.0 (s.e. 0.81), 52.1, 51.3 and 47.7 (s.e. 0.38), 114.4, 109.0 and 107.0 (s.e. 0.65) and 76.7, 71.9 and 71.4 (s.e. 0.44) cm for HO, FR and CH, respectively. Breed differences in pistola tissue distribution between the joints were small and confined to the distal pelvic limb and ribs. There were relatively small breed differences in the distribution of pistola muscle weight between individual muscles. Body measurements were significantly greater for animals on the intensive system (bulls) than the extensive system (steers) in absolute terms, but the opposite was so when they were expressed relative to live weight. The only significant difference in relative carcass measurements between the production systems was for carcass depth, which was lower for the intensive compared with the extensive system. Increasing slaughter weight significantly increased all carcass measurements in absolute terms but reduced them relative to weight. It is concluded that there were large differences between the breed types in body and carcass measurements, and hence in carcass shape and compactness but differences in tissue distribution were small

Non-carcass parts and carcass composition of high dairy genetic merit Holstein, standard dairy genetic merit Friesian and Charolais × Holstein-Friesian steers

peer-reviewedThe increased use of Holstein genetic material in the dairy herd has consequences for beef production. A total of 24 spring-born calves comprising 8 Holsteins (HO), 8 Friesians (FR) and 8 Charolais × Holstein-Friesians (CH) were reared from calfhood to slaughter. At the end of the second grazing season they were assigned to a 3 (breeds; HO, FR and CH) × 2 (slaughter weights; 620 and 730 kg) factorial experiment and fin¬ished indoors. After slaughter carcasses were classified for conformation and fatness, all organs and non-carcass parts were weighed, and the right side of each carcass was dissected into fat, bone and muscle. Non-carcass parts, carcass weight, kill-out propor¬tion, carcass conformation score and m. longissimus area were 405, 398 and 368 (s.e. 8.31) g/kg empty body weight, 355, 344 and 383 (s.e. 9.4) kg, 509, 520 and 545 (s.e. 8.99) g/kg, 1.0, 2.0 and 3.1 (s.e. 0.16), 7616, 7096 and 9286 (s.e. 223.4) mm2 for HO, FR and CH, respectively. Corresponding proportions of carcass muscle and fat were 631, 614 and 656 (s.e. 8.4), and 165, 200 and 165 (s.e. 10.5) g/kg. Increasing slaughter weight increased the proportion of total non-carcass parts, carcass weight, carcass fat score and fat proportion, and reduced carcass muscle and bone proportions. It is concluded that differences in kill-out proportion between the two dairy breeds was primarily due to the lower proportion of gastrointestinal tract (GIT) in FR, and the higher kill-out proportion of CH was mainly due to lower proportions of GIT, internal organs and internal fat. In terms of beef production, HO and FR were broadly comparable for most traits except carcass conformation score and carcass fat proportion, which were lower for HO. CH was superior to the dairy breeds in all important production traits

SmSP2: A serine protease secreted by the blood fluke pathogen Schistosoma mansoni with anti-hemostatic properties.

BackgroundSerine proteases are important virulence factors for many pathogens. Recently, we discovered a group of trypsin-like serine proteases with domain organization unique to flatworm parasites and containing a thrombospondin type 1 repeat (TSR-1). These proteases are recognized as antigens during host infection and may prove useful as anthelminthic vaccines, however their molecular characteristics are under-studied. Here, we characterize the structural and proteolytic attributes of serine protease 2 (SmSP2) from Schistosoma mansoni, one of the major species responsible for the tropical infectious disease, schistosomiasis.Methodology/principal findingsSmSP2 comprises three domains: a histidine stretch, TSR-1 and a serine protease domain. The cleavage specificity of recombinant SmSP2 was determined using positional scanning and multiplex combinatorial libraries and the determinants of specificity were identified with 3D homology models, demonstrating a trypsin-like endopeptidase mode of action. SmSP2 displayed restricted proteolysis on protein substrates. It activated tissue plasminogen activator and plasminogen as key components of the fibrinolytic system, and released the vasoregulatory peptide, kinin, from kininogen. SmSP2 was detected in the surface tegument, esophageal glands and reproductive organs of the adult parasite by immunofluorescence microscopy, and in the excretory/secretory products by immunoblotting.Conclusions/significanceThe data suggest that SmSP2 is secreted, functions at the host-parasite interface and contributes to the survival of the parasite by manipulating host vasodilatation and fibrinolysis. SmSP2 may be, therefore, a potential target for anti-schistosomal therapy

Tissue-specific expression of high-voltage-activated dihydropyridine-sensitive L-type calcium channels

The cloning of the cDNA for the α1 subunit of L-type calcium channels revealed that at least two genes (CaCh1 and CaCh2) exist which give rise to several splice variants. The expression of mRNA for these α1 subunits and the skeletal muscle α2/δ, β and γ subunits was studied in rabbit tissues and BC3H1 cells. Nucleic-acid-hybridization studies showed that the mRNA of all subunits are expressed in skeletal muscle, brain, heart and aorta. However, the α1-, β- and γ-specific transcripts had different sizes in these tissues. Smooth muscle and heart contain different splice variants of the CaCh2 gene. The α1, β and γ mRNA are expressed together in differentiated but not in proliferating BC3H1 cells. A probe specific for the skeletal muscle α2/δ subunit did not hybridize to poly(A)-rich RNA from BC3H1 cells. These results suggest that different splice variants of the genes for the α1, β and γ subunits exist in tissues containing L-type calcium channels, and that their expression is regulated in a coordinate manner