6 research outputs found
S âArylâ l âcysteine sulphoxides and related organosulphur compounds alter oral biofilm development and AI â2âbased cellâcell communication
Aims To design and synthesize a library of structurally related, small molecules related to homologues of compounds produced by the plant Petiveria alliacea and determine their ability to interfere with AI â2 cellâcell communication and biofilm formation by oral bacteria. Many human diseases are associated with persistent bacterial biofilms. Oral biofilms (dental plaque) are problematic as they are often associated with tooth decay, periodontal disease and systemic disorders such as heart disease and diabetes. Methods and Results Using a microplateâbased approach, a bioâinspired small molecule library was screened for antiâbiofilm activity against the oral species Streptococcus mutans UA 159 , Streptococcus sanguis 10556 and Actinomyces oris MG 1. To complement the static screen, a flowâbased BioFlux microfluidic system screen was also performed under conditions representative of the human oral cavity. Several compounds were found to display biofilm inhibitory activity in all three of the oral bacteria tested. These compounds were also shown to inhibit bioluminescence by Vibrio harveyi and were thus inferred to be quorum sensing ( QS ) inhibitors. Conclusion Due to the structural similarity of these compounds to each other, and to key molecules in AI â2 biosynthetic pathways, we propose that these molecules potentially reduce biofilm formation via antagonism of QS or QS ârelated pathways. Significance and Impact of the Study This study highlights the potential for a nonâantimicrobialâbased strategy, focused on AI â2 cellâcell signalling, to control the development of dental plaque. Considering that many bacterial species use AI â2 cellâcell signalling, as well as the increased concern of the use of antimicrobials in healthcare products, such an antiâbiofilm approach could also be used to control biofilms in environments beyond the human oral cavity.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/109321/1/jam12616.pd