A model for the future: Ecosystem services provided by the aquaculture activities of Veta la Palma

En prensa2,041

Probiotic supplementation influences the diversity of the intestinal microbiota during early stages of farmed Senegalese sole (Solea senegalensis, Kaup, 1858)

Ingestion of bacteria at early stages results in establishment of a primary intestinal microbiota which likely undergoes several stages along fish life. The role of this intestinal microbiota regulating body functions is crucial for larval development. Probiotics have been proved to modulate this microbiota and exert antagonistic effects against fish pathogens. In the present study, we aimed to determine bacterial diversity along different developmental stages of farmed Senegalese sole (Solea senegalensis) after feeding probiotic (Shewanella putrefaciens Pdp11) supplemented diet for a short period (10–30 days after hatching, DAH). Intestinal lumen contents of sole larvae fed control and probiotic diets were collected at 23, 56, 87, and 119 DAH and DNA was amplified using 16S rDNA bacterial domain-specific primers. Amplicons obtained were separated by denaturing gradient gel electrophoresis (DGGE), cloned, and resulting sequences compared to sequences in GenBank. Results suggest that Shewanella putrefaciens Pdp11 induces a modulation of the dominant bacterial taxa of the intestinal microbiota from 23 DAH. DGGE patterns of larvae fed the probiotic diet showed a core of bands related to Lactobacillus helveticus, Pseudomonas acephalitica, Vibrio parahaemolyticus,and Shewanella genus, together with increased Vibri o genus presence. In addition, decreased number of clones related to Photobacterium damselae subsp piscicida at 23 and 56 DAH was observed in probiotic-fed larvae. A band corresponding to Shewanella putrefaciens Pdp11 was sequenced as predominant from 23 to 119 DAH samples, confirming the colonization by the probiotics. Microbiota modulation obtained via probiotics addition emerges as an effective tool to improve Solea senegalensis larviculture.En prens

Zooplankton community composition at the Guadalquivir estuary

Trabajo presentado en ECSA 56 (Unbounded boundaries and shifting baselines: estuaries and coastal seas in a rapidly changing world), celebrado en Londres del 6 al 9 de septiembre de 2015.N

High Tolerance of Senegalese sole (S. senegalensis Kaup, 1858) larvae to a low salinity culture medium.

&nbsp;Adjusting culture salinity to optimun physiological requirements is needed for the full aquaculture development of any species. Information concerning to salinity and larval cultivation conditions in sole does not exist at present. The possibility of rearing sole larvae in brackish waters with optimum results would constitute an advantage to industry, facilitating managing practices in hatchery facilities. The aim of present work was to analyze growth, survival anf feed ingestion of larvae reared at a salinity of 10 gl-1 (S=10 psu) from first feeding, 2 days after hatching (DAH) until completing metamorphosis (DAH 21).&nbsp;Adjusting culture salinity to optimun physiological requirements is needed for the full aquaculture development of any species. Information concerning to salinity and larval cultivation conditions in sole does not exist at present. The possibility of rearing sole larvae in brackish waters with optimum results would constitute an advantage to industry, facilitating managing practices in hatchery facilities. The aim of present work was to analyze growth, survival anf feed ingestion of larvae reared at a salinity of 10 gl-1 (S=10 psu) from first feeding, 2 days after hatching (DAH) until completing metamorphosis (DAH 21).</p

Evolución espacio-temporal de la comunidad de zooplancton en el estuario del Guadalquivir

Trabajo presentado en el VIII Simposio sobre el Margen Ibérico Atlántico (MIA15), celebrado en Málaga del 21 al 23 de septiembre de 2015.Peer reviewe

Identification and quantification and fatty acids analysis of the phytoplankton community of Guadalquivir influence area at the Gulf of Cadiz

Trabajo presentado en ECSA 56 (Unbounded boundaries and shifting baselines: estuaries and coastal seas in a rapidly changing world), celebrado en Londres del 6 al 9 de septiembre de 2015.N

Flow regime in a restored wetland determines trophic links and species composition in the aquatic macroinvertebrate community

En prens

Cryopreservation of algae

Cryopreservation has been successfully used in the banking and maintenance of cultures of microorganisms, from bacteria to yeasts, since the onset of cryobiology. Biobanking of marine biological resources is crucial for development of scientific knowledge as researchers rely on guaranteed access to reliable, stable resources. Culture collections play a key role in the provision of marine biological resources as they ensure long-term ex situ storage of biological resources that are made available for public and private sector research and education. In this chapter, we provide protocols for cryopreservation of different types of algae cultures.European Marine Biological Resource Centre Biobank | Ref. EAPA_501/2016INTERREG-IV-Atlantic project EBB (European Blue Biobank