Alpha-taxonomy in the cricetid rodent Neomicroxus, a first assessment

Neomicroxus, a recently named genus, comprises small-bodied cricetid rodents patchily distributed in high-Andean ranges from Ecuador to Venezuela. Currently, two species of Neomicroxus are recognized, N. bogotensis, endemic to the Cordillera Oriental in Colombia and Cordillera de Mérida and Páramo de Tamá in Venezuela, and N. latebricola that occurs northern Andes of Ecuador. The genus is among the most poorly understood Neotropical rodents and to date no formal assessment about its alpha taxonomy was conducted. Based on DNA evidence of the first portion of the mitochondrial cytochrome b gene (cytb) and the first exon of the interphotoreceptor retinoid binding protein (IRBP), as well as craniodental measurements,we explored the divergence degree, genetic structure and phyletic relationships of the two species currently allocated under Neomicroxus. Our analyses support the monophyly of the genus as well as its uncertain tribal affiliation. Neomicroxus was retrieved as structured in two main branches, in agreement with the traditional recognition of two species. The populations referred to N. bogotensis exhibit deep divergence values (> 6 %) pointing to the existence of undescribed species under its concept. In contrast, populations of N. latebricola show a shallow genetic structure although implying recognizable geographical breaks. A moderate degree of genetic and morphological differentiation supports a new subspecies for the western populations of N. latebricola. Our contribution is the first attempt to better understanding the alpha taxonomy of Neomicroxus, highlighting the importance of the geographic complexity as a barrier to the genetic flow in N. bogotensis and the significance of the subspecies concept to formalize the geographic variation recovered in N. latebricola.Neomicroxus, un género recientemente nominado, agrupa roedores cricétidos de pequeño tamaño distribuidos en zonas altas de los Andes, desde Ecuador hasta Venezuela. Actualmente, se reconocen dos especies, N. bogotensis endémica de la Cordillera Oriental de Colombia, Cordillera de Mérida y Páramo de Tamá en Venezuela, y N. latebricola, que ocupa el norte de los Andes en Ecuador. Este género se encuentra entre los roedores neotropicales menos conocidos y, hasta la fecha, no se ha realizado ninguna evaluación formal sobre su taxonomía alfa. Basados en secuencias de ADN de la primera porción del gen mitocondrial del citocromo b (cytb) y el primer exón de la proteína de unión del interfotoreceptor del retinoide (IRBP) e información métrica cráneo-dentaria, exploramos el grado de divergencia, estructura genética y relaciones filogenéticas de las dos especies actualmente asignadas bajo Neomicroxus. Nuestros análisis apoyan la monofilia del género como así también su afiliación tribal incierta. Se recuperaron dos clados principales en concordancia con las especies reconocidas dentro del género. Las poblaciones referidas a N. bogotensis muestran valores de divergencia profunda (> 6 %) que sugieren la existencia de una especie no descrita. En contraste, las poblaciones de N. latebricola muestran una estructura genética somera pero que implica quiebres geográficos reconocibles. La diferenciación genética y morfológica moderada apoyan la existencia de una nueva subespecie para las poblaciones occidentales de N. latebricola. Nuestra contribución es el primer intento de comprender la taxonomía alfa de Neomicroxus, destacando la importancia de la complejidad geográfica como barrera para el flujo genético en N. bogotensis y la importancia del concepto de subespecie para formalizar la variación geográfica observada en N. latebricola.Fil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Curay, Jenny. Instituto Nacional de Biodiversidad; EcuadorFil: Brito, Jorge. Instituto Nacional de Biodiversidad; EcuadorFil: Colmenares-Pinzón, Javier E.. Universidad Industrial Santander; ColombiaFil: Pardiñas, Ulises Francisco J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina. Instituto Nacional de Biodiversidad; Ecuado

Morphological variation of Oligoryzomys longicaudatus magellanicus (Rodentia: Sigmodontinae) west of the Southern Patagonian Ice Fields in Chile

Oligoryzomys es uno de los géneros de roedores más ampliamente distribuido en el territorio chileno. Se describe la variabilidad morfológica de individuos provenientes de localidades al oeste de Campos de Hielo Patagónico Sur entre los 48° S y 51° S. Análisis uni y multivariados realizados sobre la base de mediciones del cuerpo, mandíbula y cráneo no mostraron diferencias significativas por sexo, pero sí por procedencia geográfica. Los individuos insulares presentaron valores mayores para casi todos los caracteres evaluados, lo que podría corresponderse con la ausencia de predadores y competidores. Morfológica y distribucionalmente, los individuos analizados son asignados a la subespecie Oligoryzomys longicaudatus magellanicus del extremo sur del continente.Oligoryzomys is one of the most widely distributed mammal genera in Chile. We describe morphological variation among specimens from west of the Southern Patagonian Ice Fields, between 48° S and 51° S. Univariate and multivariate analysis showed no significant dif­ferences between sexes, but uncovered significant geographical variation. Island specimens had higher values for almost all characters evaluated, which could correspond to the absence of predators and competitors. Based on morphological and distributional information, we suggest that individuals analyzed belong to the subspecies Oligoryzomys longicaudatus magellanicus.Fil: Guzmán, Jonathan A.. Bayerische Staatssammlung für Paläontologie und Geologie; Alemania. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía; ChileFil: Ortiz, Juan Carlos. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; ChileFil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentin

Comprehensive total evidence phylogeny of chinchillids (Rodentia, Caviomorpha): Cheek teeth anatomy and evolution

Rodents are the most diverse order of extant mammals, and caviomorph rodents, or New World hystricognaths, have a remarkable morphological disparity and a long fossil record that begins in the Eocene. Chinchilloidea is a poorly understood clade within Caviomorpha, from an evolutionary and phylogenetic perspective. It includes the extant families Chinchillidae and Dinomyidae, the extinct Neoepiblemidae and Cephalomyidae, and several extinct chinchilloids without a clear phylogenetic position, like Eoincamys, Borikenomys, Chambiramys, Ucayalimys, Incamys, Saremmys, Garridomys and Scotamys. The family Chinchillidae includes the extant Chinchilla and Lagidium, grouped in Chinchillinae, and the only living Lagostominae, Lagostomus maximus. Among extinct chinchillids, Eoviscaccia (early Oligocene-early Miocene of Argentina, Bolivia and Chile), Prolagostomus (early-middle Miocene of Argentina, Bolivia and Chile) and Pliolagostomus (early-middle Miocene of Argentina) are the only genera originally described as members of the family. Based on the study of specimens with unworn or little-worn cheek teeth, belonging to extinct and extant taxa, we propose homologies of the cheek teeth structures and perform a combined molecular and morphological phylogenetic analysis including extinct and extant taxa of all families of Chinchilloidea and all genera of Chinchillidae. Our phylogenetic analysis recovered three major lineages in the evolutionary history of Chinchilloidea. The first major lineage is composed of the extant taxa Chinchilla, Lagidium and Lagostomus, and the extinct genera Eoviscaccia, Prolagostomus, Pliolagostomus, Garridomys, Incamys, Loncolicu and Saremmys. Cephalomyid (Banderomys, Cephalomys, Litodontomys, Soriamys) and neoepiblemid (Neoepiblema, Perimys, Phoberomys, Scotamys) genera are part of the second major lineage, while dinomyids such as Dinomys, Drytomomys, Scleromys, ‘Scleromys’ and Tetrastylus constitute the third major lineage within Chinchilloidea. The phylogenetic position of some taxa previously considered as incertae sedis chinchilloids or without a clear suprageneric group (i.e. Incamys, Saremmys, Garridomys and Loncolicu) show that they belong to pan-Chinchillidae and conform the stem Chinchillidae along with Eoviscaccia. The euhypsodont crown Chinchillidae includes the living subfamilies Chinchillinae and Lagostominae. Dinomyidae and Eoincamys pascuali are recovered as the sisters of a major clade composed by ‘Cephalomyidae’+Neopiblemidae and pan-Chinchillidae, and Chambiramys sylvaticus occupies a basal position to the same clade. Four major radiation events are identified in the evolutionary history of Chinchilloidea. The analysis of new morphological characters linked with molecular evidence as well as the addition of taxa of uncertain or unstable phylogenetic position or not considered in previous studies allowed us resolve part of the relationships within Chinchilloidea, particularly that of Chinchillidae, supporting preceding morphological hypotheses.Fil: Rasia, Luciano Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Paleontología Vertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Candela, Adriana Magdalena. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Paleontología Vertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentin

A matter of weight: Critical comments on the basic data analysed by Maestri et al. (2016) in Journal of Biogeography, 43, 1192–1202

Recently, Maestri, Luza, et al. () assessed the effect of ecology and phylogeny on body size variation in communities of South American Sigmodontinae rodents. Regrettably, a cursory analysis of the data and the phylogeny used to address this question indicates that both are plagued with inaccuracies. We urge “big data” users to give due diligence at compiling data in order to avoid developing hypotheses based on insufficient or misleading basic information.Fil: Pardiñas, Ulises Francisco J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Salazar Bravo, Jorge. Texas Tech University; Estados Unido

The availability, authorships and dates of tribal names in the Sigmodontinae (Rodentia, Cricetidae) current classification

SIGMODONTINAE, a diverse subfamily including 106 genera of American Rodents, is currently divided in eleven tribes that still need to be refined based on recently generated phylogenetic hypotheses. Several published names of tribes do not conform to one or more Rules of the International Code of Zoological Nomenclature. In particular, the first arrangement of SIGMODONTINAE genera in tribes has been overlooked, the different requirements for availability of names proposed before 1931 and after 1930 were not taken into account for at least five names, the requirement that a family-group name be used as valid in the publication where proposed, and the unavailability of names proposed conditionally after 1960 were also ignored on several occasions. Our analysis tries to disclose and fix such nomenclatural problems keeping current usage as much as possible. A new name is proposed for a tribe that currently has been designated only by an unavailable name.Fil: Cazzaniga, Néstor Jorge. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Pardiñas, Ulises Francisco J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina. Instituto Nacional de Biodiversidad; Ecuado

Phylogeny of the tribe Abrotrichini (Cricetidae, Sigmodontinae): Integrating morphological and molecular evidence into a new classification

The tribe Abrotrichini (five genera and 14 living species) is a small clade within the speciose subfamily Sigmodontinae (Rodentia, Cricetidae), representing one of the extant successful radiations of mammals at southern high latitudes of the Neotropics. Its distribution is mostly Andean, reaching its greatest diversity in southern Argentina and Chile. We evaluate the phylogenetic relationships within this tribe through parsimony and Bayesian approaches based on 99 morphological characters (including 19 integumental characters, 38 skull characters, 31 dental characters, three postcranial skeletal characters, seven from the male accessory glands and phallus and one from the digestive system) and six molecular markers (one mitochondrial and five nuclear). We include representatives of all, except one, of the currently recognized species of living Abrotrichini plus one fossil form. Based on total evidence, we recovered a primary division between the genus Abrothrix and a group including the long-clawed Abrotrichini, Chelemys, Geoxus, Notiomys and Pearsonomys. Both clades are recognized and named here as subtribes. The large degree of morphological variation observed within Abrothrix suggests that species in the genus fall into four groups, which we recognize as subgenera. In addition, the two known species of Chelemys do not form a monophyletic group, and Geoxus was recovered as paraphyletic with respect to Pearsonomys. To reconcile classification and phylogenetics, we describe a new genus for Chelemys macronyx and include Pearsonomys as a junior synonym of Geoxus. Our results highlight the importance of both morphology and molecules in resolving the phylogenetic relationships within this tribe. Based on biogeographical analyses, we hypothesize that Abrotrichini originated in south-western South America by vicariance and then diversified mostly by successive dispersal events.Fil: Teta, Pablo Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Patterson, Bruce. Field Museum of Natural History; Estados UnidosFil: Pardiñas, Ulises Francisco J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentin

Molecular detection of Bartonella in fleas (Hexapoda, Siphonaptera) collected from wild rodents (Cricetidae, Sigmodontinae) from Argentina

Bartonella are facultative intracellular Gram-negative bacteria, transmitted mainly by hematophagous arthropods, and the rodents act as a natural reservoir. Different species of Bartonella associated with rodents have been implicated as causing human disease. Studies from Argentina are scarce and no Bartonella from fleas have been reported previously. The present study investigated the presence of Bartonella spp. in fleas associated with sigmodontine rodents in four localities of the Santa Cruz Province, Argentina. In total, 51 fleas (four species) were analysed of which 41.2% were found to be positive for the gltA gene fragment via a nested polymerase chain reaction. All positive fleas were of the species Neotyphloceras crackensis from three different localities. Eight of the 21 amplified samples were sequenced, and the presence of three different genotypes was detected with an identity of 95.5–98.8% amongst themselves. Bartonella genotypes from American rodents and rodent fleas were recovered in a monophyletic group. Similarly, most of the Peruvian and all Argentinean variants constitute a natural group sister of the American remainder. The importance of the Bartonella spp. with respect to public health is unknown, although future studies could provide evidence of the possible involvement of N. crackensis in the Bartonella transmission cycles.Fil: Cicuttin, Gabriel Leonardo. Instituto de Zoonosis Luis Pasteur; ArgentinaFil: de Salvo, María Nazarena. Instituto de Zoonosis Luis Pasteur; ArgentinaFil: Sánchez, Juliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentin

Phylogeography of Loxodontomys micropus with comments on the alpha taxonomy of Loxodontomys (Cricetidae: Sigmodontinae)

Loxodontomys micropus is a rodent that is widely distributed in Andean and Patagonian Argentina and Chile. This range covers a heterogeneous area that has been influenced by geologic and palaeoclimatic events, such as the glaciations during the Neogene. To investigate the genetic structure, phylogeographic pattern, and biogeographic history of this sigmodontine rodent we analyzed a 801-base-pair fragment of the mitochondrial genome (cytochrome-b gene) of 87 specimens from 24 localities from Argentina and Chile. Results indicate that L. micropus has a shallow genealogy that is geographically structured and is a taxon characterized by an historical population expansion. We discuss the distribution of the genetic variation of L. micropus in relation to population history and the concordance with other codistributed sigmodontine rodents. On the basis of molecular evidence, we suggest that the L. pikumche, corresponding to the second extant species of the genus, could be a junior synonym of L. micropusFil: Cañón Valenzuela, Carola Patricia. Universidad de Concepción; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: D Elía, Guillermo. Universidad Austral de Chile; ChileFil: Pardiñas, Ulises Francisco J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; ArgentinaFil: Lessa, Enrique P.. Universidad de la República; Urugua

A multilocus perspective on the phylogenetic relationships and diversification of rodents of the tribe Abrotrichini (Cricetidae: Sigmodontinae)

Abrotrichini is a recently defined and diagnosed tribe of Sigmodontinae with a complex taxonomy. Abrotrichine genera, Abrothrix (including Chroeomys), Chelemys, Geoxus, Notiomys and Pearsonomys, are mostly distributed in the central and southern Andes and adjacent lowlands and show terrestrial and fossorial habits. Recent studies have evidenced some incongruence between current taxonomy and abrotrichine phylogeny, such as the polyphyly of Chelemys and paraphyly of Geoxus respect to Pearsonomys. We used DNA sequence data of six loci (one mitochondrial and five nuclear) to resolve the relationships within the tribe. Independent and combined analyses of these loci were carried out using parsimony, maximum likelihood and Bayesian inference. Estimates of divergence time of the main lineages of abrotrichines were calculated with a molecular clock using as calibration, a fossil recently found. The concatenated data set increased the resolution and defined the relationships within the tribe. Our phylogenetic analyses corroborate that Abrothrix is sister of a clade containing the long-clawed abrotrichines. We recovered two main clades within Abrothrix that match morphologic variation and geographic distribution of its species. In addition, we corroborated the lack of monophyly of Chelemys and the lack of monophyly of Geoxus. We discuss different taxonomic scenarios to abrotrichine classification reflects the phylogenetic relationships obtained in this study. Our molecular clock estimated the Abrotrichini crown age to be around the early Pliocene (4.4 Ma) and suggest that the tribe diversified over a short period of time.Fil: Cañón Valenzuela, Carola Patricia. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanografía. Departamento de Zoología; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mir, Daiana. Universidad de la República. Facultad de Ciencias; UruguayFil: Pardiñas, Ulises Francisco J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; ArgentinaFil: Lessa, Enrique P.. Universidad de la República. Facultad de Ciencias; UruguayFil: D´Elía, Guillermo. Universidad Austral de Chile; Chil

Morphological disparity in a hyperdiverse mammal clade: A new morphotype and tribe of Neotropical cricetids

Rhagomys is a genus of South American cricetids composed of three living species. They occur in eastern Andean and lowland tropical forests. Since description of this genus more than a century ago, it was treated as an enigmatic form within Sigmodontinae, varyingly thought to be allied to different suprageneric groups, with several scholars highlighting their presumptive autapomorphies. However, this morphologically based controversy was resolved with the advent of molecular data, indicating a weak affiliation to Thomasomyini. Here we resume the anatomical scrutiny of Rhagomys based on new evidence. New data, including CT-scan craniodental exploration and an ICAMER molar analysis, reinforce the uniqueness of this cricetid in the subfamily. In addition, we have enlarged and reworked the genetic data, composed of mitochondrial and nuclear markers, supporting not only the monophyly of Rhagomys, but also its resolution as a distinct branch of the sigmodontine radiation. To accommodate these results, we describe a new tribe for Rhagomys. The recognized clade is the most phenotypically differentiated of the Oryzomyalia and inaugurates a new arboreal morphotype. We hypothesize that Rhagomys has a set of anatomical features used to exploit understory resources by primarily insectivorous mice.Fil: Pardiñas, Ulises Francisco J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; Argentina. Instituto Nacional de Biodiversidad; EcuadorFil: Tinoco, Nicolás. Pontificia Universidad Catolica del Ecuador. Facultad de Cs Exactas y Naturales; EcuadorFil: Barbiere, Franck. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ronez, Christophe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Cañón Valenzuela, Carola Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Diversidad y Evolución Austral; ArgentinaFil: Lessa, Gisele. Universidade Federal de Viçosa; BrasilFil: Koch, Claudia. Zoologisches Forschungsmuseum Alexander Koenig; AlemaniaFil: Brito, Jorge. Instituto Nacional de Biodiversidad; Ecuado