Mineral Type and Solution Chemistry Affect the Structure and Composition of Actively Growing Bacterial Communities as Revealed by Bromodeoxyuridine Immunocapture and 16S rRNA Pyrosequencing

© 2016, Springer Science+Business Media New York. Understanding how minerals affect bacterial communities and their in situ activities in relation to environmental conditions are central issues in soil microbial ecology, as minerals represent essential reservoirs of inorganic nutrients for the biosphere. To determine the impact of mineral type and solution chemistry on soil bacterial communities, we compared the diversity, composition, and functional abilities of a soil bacterial community incubated in presence/absence of different mineral types (apatite, biotite, obsidian). Microcosms were prepared containing different liquid culture media devoid of particular essential nutrients, the nutrients provided only in the introduced minerals and therefore only available to the microbial community through mineral dissolution by biotic and/or abiotic processes. By combining functional screening of bacterial isolates and community analysis by bromodeoxyuridine DNA immunocapture and 16S rRNA gene pyrosequencing, we demonstrated that bacterial communities were mainly impacted by the solution chemistry at the taxonomic level and by the mineral type at the functional level. Metabolically active bacterial communities varied with solution chemistry and mineral type. Burkholderia were significantly enriched in the obsidian treatment compared to the biotite treatment and were the most effective isolates at solubilizing phosphorous or mobilizing iron, in all the treatments. A detailed analysis revealed that the 16S rRNA gene sequences of the OTUs or isolated strains assigned as Burkholderia in our study showed high homology with effective mineral-weathering bacteria previously recovered from the same experimental site

Use of Biomarkers in the Evaluation and Treatment of Hypertensive Patients

The current definition of hypertension is based on blood pressure values, and blood pressure also drives treatment decisions, is the most important treatment monitoring tool and helps estimating risk of hypertension related organ damage. In an era of precision medicine additional biomarkers are needed in the diagnosis and management of patients with hypertension. In this review we outline the areas in which functional, imaging and circulating biomarkers could help in a more individualised definition of hypertension and associated risk. We will cover biomarkers for diagnosis; of pathophysiology and prediction of hypertension; response to treatment, organ damage; and to monitor treatment. A clear focus is on the vasculature, the heart and the kidneys, whereas we see a need to further develop biomarkers of cerebral function in order to diagnose cognition deficits and monitor changes in cognition in the future to support addressing the growing burden of hypertension associated vascular dementia

Uso de álcool, tabaco e outras drogas por adolescentes escolares em município do Sul do Brasil Alcohol, tobacco, and other drug use by teenage students in a city in Southern Brazil

Este trabalho teve como objetivo estudar o comportamento dos estudantes do ensino público municipal de Gravataí, Rio Grande do Sul, Brasil, em relação ao consumo de álcool, tabaco e outras drogas, investigando fatores associados. Foi utilizado delineamento transversal com amostra aleatória representativa de 1.170 escolares da 7ª série e estratificada por região do município. As associações entre desfechos e fatores em estudo foram testadas com regressão de Cox bivariada, modificada para estudos transversais. Foram encontrados, respectivamente, 60,7%, 16,9% e 2,4% de uso na vida de bebida alcoólica, tabaco e outras drogas. Nos últimos trinta dias, o álcool também apresentou a maior prevalência (33%), seguido pelo tabaco (4,4%) e outras drogas (0,6%). O estudo revelou a importância da família e dos amigos na experimentação das substâncias pelos jovens. A prevalência do uso de tabaco e álcool nos últimos trinta dias esteve associada à presença de sentimentos de tristeza, solidão, dificuldade para dormir e ideação suicida. O consumo de outras drogas esteve associado ao sentimento de solidão e ideação suicida.<br>This study analyzes alcohol, tobacco, and other drug use by seventh-graders in municipal schools in the city of Gravataí, Rio Grande do Sul State, Brazil, and associated factors. A cross-sectional school survey was conducted in a representative random sample of 1,170 seventh-graders from municipal schools. The bivariate Cox regression model, modified for cross-sectional studies, was used to evaluate associations between specific factors and outcomes. Prevalence rates for lifetime consumption of alcohol, tobacco, and other drugs were 60.7%, 16.9%, and 2.4%, respectively. Consumption rates in the previous 30 days were: alcohol, 33%; tobacco, 4.4%; and other drugs, 0.6%. The study showed an association with substance use by family and friends. Prevalence of tobacco and alcohol use during the previous 30 days was associated with feelings of sadness, loneliness, insomnia, and suicidal ideation. Use of other drugs was associated with loneliness and suicidal ideation