11 research outputs found
TiF4 and NaF varnishes as anti-erosive agents on enamel and dentin erosion progression in vitro
Objective This study assessed the effect of fluoride varnishes on the progression of tooth erosion in vitro. Material and Methods: Forty-eight enamel and 60 root dentin samples were previously demineralized (0.1% citric acid, pH 2.5, 30 min), leading to a baseline and erosive wear of 12.9 and 11.4 µm, respectively. The samples were randomly treated (6 h) with a 4% TiF4 varnish (2.45%F-, pH 1.0), a 5.42% NaF varnish (2.45%F-, pH 5.0), a placebo varnish and no varnish (control). The samples were then subjected to erosive pH cycles (4x90 s/day in 0.1% citric acid, intercalated with artificial saliva) for 5 days. The increment of the erosive tooth wear was calculated. In the case of dentin, this final measurement was done with and without the demineralized organic matrix (DOM). Enamel and dentin data were analyzed using ANOVA/Tukey’s and Kruskal-Wallis/Dunn tests, respectively (
TiF4 and NaF varnishes as anti-erosive agents on enamel and dentin erosion progression in vitro
Comparison between the effect of TiF4 and NaF varnishes and solutions on bovine enamel demineralization and remineralization in situ
Este estudo teve como objetivo avaliar do efeito da aplicação tópica de um verniz e solução experimental de TiF4, comparando-os ao verniz e solução de NaF, sobre o processo de des-remineralização do esmalte dentário in situ. Para tal, 132 blocos de esmalte bovino (4x4mm) foram selecionados pela microdureza de superfície, sendo que em metade deles foi produzida lesão de cárie artificial em solução desmineralizante (pH 5,0, 6 dias). Na seqüência, foi determinada a microdureza de superfície pós-lesão. A outra metade dos blocos permaneceu hígida. Onze voluntários participaram de 3 fases cruzadas (14 dias cada, washout de 7 dias), boca-dividida e cegas, nas quais utilizaram aparelhos palatinos com 4 amostras de esmalte por fase (2 hígidas e 2 desmineralizadas), sendo que cada tipo de amostra recebeu um tipo de tratamento (verniz ou solução). Em todas as amostras permitiu-se o acúmulo de placa dentária. Em uma fase (Fase A), foram aplicados agentes a base de NaF a 5,42% (2,45% F- e pH 5,0), em outra fase (Fase B), foram utilizados agentes a base de TiF4 a 4% (2,45% F- e pH 1,0), e na última fase (Fase C) foram utilizados agentes placebos (verniz sem flúor pH 5,0 e sem tratamento). Os tratamentos foram realizados in vitro (verniz-6 h e solução-1 minuto). Durante o período in situ, os voluntários aplicaram solução de sacarose a 20%, 8x5min ao dia, sobre os blocos hígidos, para provocar a desmineralização. Já para os blocos desmineralizados nenhum procedimento foi realizado, para permitir a remineralização. A microdureza de superfície final foi realizada, para o cálculo da porcentagem de variação de microdureza de superfície, assim como a microdureza longitudinal e microradiografia transversal, para o cálculo das perdas de microdureza e volume mineral integradas, respectivamente. Os dados foram submetidos à ANOVA-RM/ Tukey-Kramer, teste t pareado e ANOVA a dois critérios/Bonferroni (p<0,05). Houve uma redução significativa da desmineralização superficial e um aumento significativo da remineralização superficial do esmalte in situ pelos tratamentos com verniz e solução de TiF4, em comparação aos controles. Pôde-se constatar ainda que os tratamentos com o verniz de NaF, verniz de TiF4 e solução de TiF4 foram efetivos na redução da desmineralização subsuperficial in situ. No entanto, observou-se que o protocolo experimental não permitiu uma adequada remineralização de subsuperfície in situ. Com base nos resultados, pode-se concluir que o verniz e a solução de TiF4 parecem ser promissores na prevenção da desmineralização, porém em relação à remineralização mais estudos in situ são necessários.The aim of this study was to evaluate the effect of the experimental TiF4 varnish and solution, comparing with NaF solution and varnish, on dental enamel de-remineralization in situ. For that, 132 bovine enamel samples (4x4 mm) were selected using surface microhardness, and half of them were submitted to artificial caries lesion formation using a demineralizing solution (pH 5.0, 6 days). The surface microhardness after the demineralization was measured. The other half of the samples remained sound. Eleven volunteers took part in this cross-over, split-mouth and double-blind study performed in 3 phases of each 14 days (washout of 7 days). Four enamel samples were worn in palatal appliances (2 sound and 2 demineralized), being each type of sample submitted to one treatment (varnish or solution). The dental plaque accumulation was allowed on all samples. In phase A, 5.42% NaF varnish/solution was applied (2.45% F-, pH 5.0); in phase B, 4% TiF4 varnish/solution was used (2.45% F-, pH 1.0) and at the last phase (phase C) the placebo treatments were performed (placebo varnish pH 5.0 and no-treatment). The treatments were performed in vitro (varnish-6h and solution-1min). During the in situ phase, the volunteers dripped a solution containing 20% weight/volume (w/v) sucrose on the sound samples for 5 min, 8 times a day, to provoke demineralization. While on the demineralized samples nothing was done, in order to allow remineralization. The final surface microhardness was analyzed for the calculation of the % of surface microhardness change, as well as the cross-sectional hardness and the transversal microradiography, for the calculation of the integrated loss of microhardness and mineral volume, respectively. The data were submitted to RM-ANOVA/ Tukey-Kramer, t test and 2-way ANOVA/ Bonferroni (p<0.05). There was a significant reduction of the surface enamel demineralization and increase of surface remineralization by the treatments with TiF4 varnish and solution compared to control in situ. The treatments with NaF varnish, TiF4 varnish and solution were able to reduce the subsurface demineralization in situ. However, the experimental protocol did not allow the subsurface remineralization in situ. Based on the results, it can be concluded that the TiF4 varnish and solution seem to be promising products for preventing demineralization, but for the remineralization more studies need to be conducted
Evaluation of fluoride release from experimental TiF4 and NaF varnishes in vitro
Fluoride varnishes play an important role in the prevention of dental caries, promoting the inhibition of demineralization and the increase of remineralization. Objective: This study aimed to analyze the amount of fluoride released into water and artificial saliva from experimental TiF4 and NaF varnishes, with different concentrations, for 12 h. Material and Methods: Fluoride varnishes were applied on acrylic blocks and then immersed in 10 ml of deionized water and artificial saliva in polystyrene bottles. The acrylic blocks were divided in seven groups (n=10): 1.55% TiF4 varnish (0.95% F, pH 1.0); 3.10% TiF4 varnish (1.90% F, pH 1.0); 3.10% and 4% TiF4 varnish (2.45% F, pH 1.0); 2.10% NaF varnish (0.95% F, pH 5.0); 4.20% NaF varnish (1.90% F, pH 5.0); 5.42% NaF varnish (2.45% F, pH 5.0) and control (no treatment, n=5). The fluoride release was analyzed after 1/2, 1, 3, 6, 9 and 12 h of exposure. The analysis was performed using an ion-specific electrode coupled to a potentiometer. Two-way ANOVA and Bonferroni's test were applied for the statistical analysis (
The erosion and abrasion-inhibiting effect of TiF4 and NaF varnishes and solutions on enamel in vitro
Objective. Previous in vitro study has shown that TiF(4) varnish might reduce enamel erosion. No data regarding the effect of this experimental varnish on enamel erosion plus abrasion, however, are available so far. Thus, this in vitro study aimed to analyse the effect of TiF4 compared with NaF varnishes and solutions, to protect against enamel erosion with or without abrasion. Methods. Enamel specimens were pre-treated with experimental-TiF4 (2.45% F), experimentalNaF (2.45% F), NaF-Duraphat (2.26% F), and placebo varnishes; NaF (2.26% F) and TiF4 (2.45% F) solutions. Controls remained untreated. The erosive challenge was performed using a soft drink (pH 2.6) 4 u 90 s / day (ERO) and the toothbrushing abrasion (ERO+ ABR) 2 u 10 s / day, for 5 days. Between the challenges, the specimens were exposed to artificial saliva. Enamel loss was measured profilometrically (lm). Results. Kruskal-Wallis / Dunn tests showed that all fluoridated varnishes (TiF4-ERO: 0.53 +/- 0.20, ERO+ ABR: 0.65 +/- 0.19/ NaF-ERO: 0.94 +/- 0.18, ERO+ ABR: 1.74 +/- 0.37 / Duraphat-ERO: 1.00 +/- 0.37, ERO+ ABR: 1.72 +/- 0.58) were able to significantly reduce enamel loss when compared with placebo varnish (ERO: 3.45 +/- 0.41 / ERO+ ABR: 3.20 +/- 0.66) (P < 0.0001). Placebo varnish, control (ERO: 2.68 +/- 0.53 / ERO+ ABR: 3.01 +/- 0.34), and fluoridated (NaF-ERO: 2.84 +/- 0.09 / ERO+ ABR: 2.40 +/- 0.21 / TiF4-ERO: 3.55 +/- 0.59 / ERO+ ABR: 4.10 +/- 0.38) solutions did not significantly differ from each other. Conclusion. Based on the results, it can be concluded that the TiF4 varnish seems to be a promising treatment to reduce enamel loss under mild erosive and abrasive conditions in vitro.FAPESPFAPESP [Proc. 08/07105-4, 08/03727-0
TiF4 and NaF varnishes as anti-erosive agents on enamel and dentin erosion progression in vitro
Objective This study assessed the effect of fluoride varnishes on the progression of tooth erosion in vitro. Material and Methods: Forty-eight enamel and 60 root dentin samples were previously demineralized (0.1% citric acid, pH 2.5, 30 min), leading to a baseline and erosive wear of 12.9 and 11.4 µm, respectively. The samples were randomly treated (6 h) with a 4% TiF4 varnish (2.45%F-, pH 1.0), a 5.42% NaF varnish (2.45%F-, pH 5.0), a placebo varnish and no varnish (control). The samples were then subjected to erosive pH cycles (4x90 s/day in 0.1% citric acid, intercalated with artificial saliva) for 5 days. The increment of the erosive tooth wear was calculated. In the case of dentin, this final measurement was done with and without the demineralized organic matrix (DOM). Enamel and dentin data were analyzed using ANOVA/Tukey’s and Kruskal-Wallis/Dunn tests, respectively (p<0.05). Results The TiF4 (mean±s.d: 1.5±1.1 µm) and NaF (2.1±1.7 µm) varnishes significantly reduced enamel wear progression compared to the placebo varnish (3.9±1.1 µm) and control (4.5±0.9 µm). The same differences were found for dentin in the presence and absence of the DOM, respectively: TiF4 (average: 0.97/1.87 µm), NaF (1.03/2.13 µm), placebo varnish (3.53/4.47 µm) and control (3.53/4.36 µm). Conclusion The TiF4 and NaF varnishes were equally effective in reducing the progression of tooth erosion in vitro
Effect of a Single Application of TiF(4) and NaF Varnishes and Solutions Combined with Nd:YAG Laser Irradiation on Enamel Erosion in Vitro
Objective: This in vitro study aimed to analyze the influence of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on the efficacy of titanium tetrafluoride (TiF(4)) and sodium fluoride (NaF) varnishes and solutions to protect enamel against erosion. Background data: The effect of Nd:YAG laser irradiation on NaF and AmF was analyzed; however, there is no available data on the interaction between Nd:YAG laser irradiation and TiF(4). Methods: Bovine enamel specimens were pre-treated with NaF varnish, TiF(4) varnish, NaF solution, TiF(4) solution, placebo varnish, Nd:YAG (84.9 J/cm(2)), Nd:YAG prior to or through NaF varnish, Nd:YAG prior to or through TiF(4) varnish, Nd:YAG prior to or through NaF solution, Nd:YAG prior to or through TiF(4) solution, and Nd:YAG prior to or through placebo varnish. Controls remained untreated. Ten specimens in each group were then subjected to an erosive demineralization (Sprite Zero, 4x90 s/day) and remineralization (artificial saliva, between the erosive cycles) cycling for 5 days. Enamel loss was measured profilometrically (mu m). Additionally, treated but non-eroded specimens were additionally analyzed by scanning electron microscope (SEM) (each group n-2). The data were statistically analyzed by ANOVA and Tukey's post-hoc test (p < 0.05). Results: Only TiF(4) varnish (1.8 +/- 0.6 mu m), laser prior to TiF(4) varnish (1.7 +/- 0.3 mu m) and laser prior to TiF(4) solution (1.4 +/- 0.3 mu m) significantly reduced enamel erosion compared to the control (4.1 +/- 0.6 mu m). SEM pictures showed that specimens treated with TiF(4) varnish presented a surface coating. Conclusions: Nd:YAG laser irradiation was not effective against enamel erosion and it did not have any influence on the efficacy of F, except for TiF(4) solution. On the other hand, TiF(4) varnish protected against enamel erosion, without the influence of laser irradiation.LELO-USP (Special Laboratory of Lasers in Dentistry-University of Sao Paulo), Sao Paulo, Brazil[FAPESP 2007/55497-0]Edimauro de AndradePIBIC-CNPq (The National Council for Scientific and Technological Development