The effects of divergent selection for reproduction and sex on quantitative and qualitative slaughter traits in Merinos

The impact of selection of sheep for reproduction on meat traits are not evident, while genetic parameters for meat traits is absent for South African pure breeds. Quantitative and qualitative meat traits were therefore studied in progeny of two Merino selection lines that were divergently selected for number of lambs weaned per ewe joined (NLW) since 1986. The historic divergent selection resulted in two lines (High (H) and Low (L)) differing widely for NLW. Slaughter data were recorded during the routine slaughter of surplus 14-month-old ram and ewe hoggets from these lines and assessed for selection line and sex. Single-trait heritability estimates were derived for meat traits by average information restricted maximum likelihood methods. Depending on the trait, data were available for between 340 and 576 animals that were recorded between 2015 and 2018. Hoggets from the H line were heavier than their L line contemporaries, with a slightly lower ultimate pH after 48 h in the cooler and slightly darker meat. H line ewes had, on average, redder meat than the other selection line x sex groups. Single-trait heritability estimates amounted to 0.44 ± 0.16 for slaughter weight, 0.63 ± 0.15 for carcass weight, 0.34 ± 0.15 for dressing percentage, 0.25 ± 0.11 for fat depth at the 13th rib, 0.29 ± 0.11 for fat depth at the rump, 0.12 ± 0.11 for ultimate pH, 0.32 ± 0.12 for lightness, 0.11 ± 0.09 for redness, 0.04 ± 0.06 for yellowness, 0.05 ± 0.08 for cooking loss and 0.06 ± 0.07 for drip loss. Parameter estimates for initial pH and shear force of the meat went to the boundary of parameter space and were not estimable. It was concluded that selection for NLW did not compromise any of the meat traits and that most quantitative meat traits were heritable and variable, making selection for improvement of these traits feasible. Additional research is indicated on the qualitative meat traits studied.Cape Wools SA, Red Meat Research and Development SA, the Research Technology Fund, as well as from the Western Cape Agricultural Research Trust.https://www.elsevier.com/locate/smallrumreshj2022Animal and Wildlife Science

Searching for SNPs that affect sheep robustness: CYP17 SNP affects behavioural responses to psychological stress

The ability of animals to adapt to stress is not only an animal health and welfare concern, but also influences reproduction potential and robustness. An important pathway involved in the stress response is the hypothalamic-pituitary-adrenal axis (HPAA) that results in the release of cortisol from the adrenal gland. In this study the cortisol responses of South African Merinos were measured to assess HPAA responsiveness to stress and relate it to behavioural stress responses to flock-isolation. The experiment was structured according to a 2×2 statistical design, with CYP17 genotype (WT1/WT1 vs. WT1/WT2) and selection line (H-line vs. L-line) as factors. Selection line criteria was based on divergent selection for (H-line) or against (L-line) maternal multiple rearing ability, where the H-line generally outperformed the L-line in terms of reproduction, animal welfare and resistance to certain pathogens. The CYP17 genotype is involved in the biosynthesis pathway of cortisol. In the present study the CYP17 genotype showed a significant influence on behavioural stress responses, where three parameters of the flock-isolation test were affected (P<0.05), namely the number of bleats uttered, the urinating frequency and the average distance from a human operator. It is suggested that the CYP17 genotype affects behavioural responses via its effects on cortisol production, and that the SNPs located within the CYP17 genotype may have application in marker-assisted selection of sheep

Sensory and objective mutton quality characteristics of SA Merino sheep selected for and against reproductive fitness

: The effect of divergent selection for ewe multiple-rearing ability over a 15-year period on mutton quality was examined on the M. longissimus dorsi and M. semimembranosus of entire rams (R) and ewes (E) derived from two Merino lines. The selection of these two lines was based on maternal ranking values for multiple-rearing ability in a positive (P) and negative (N) line. In the negative line the replacements were based on the progeny of ewes that rear less than one lamb per joining or lambing opportunity (i.e. failed to lamb or lost all progeny born at least once). Progeny of ewes that reared more than one lamb per joining (i.e. reared twins at least once) were selected for the positive line. Ratings of sensory quality characteristics on the M. semimembranosus of the different genotype lines were obtained from a trained taste panel and related to data on physical and chemical characteristics. The moisture, total lipids, protein, ash, mineral concentrations and fatty acid composition of the M. semimembranosus were obtained. Physical parameters measured on the M. longissimus dorsi were: ultimate pH (pH48), drip loss, cooking loss and Warner-Bratzler shear force (WBS). The influence of selection line on the sensory characteristics of the mutton was generally negligible, with the exception of the sensory attribute of first bite, where meat derived from the positive line was rated to be less tender than that of the negative line contemporaries. Chemically, the meat derived from the four different groups differed significantly in moisture and lipid content. The Positive Ram (PR) group had the highest moisture (ca. 76%) and the lowest lipid (ca. 7%) concentrations, whereas the Negative Ewe (NE) group had the lowest moisture (ca. 70%) and the highest lipid (ca. 10%) concentration. No significant differences were detected in the proximate chemical composition between the P and N lines. The pH48 and WBS values showed significant differences between the four groups. The Negative Ram (NR) group had the highest pH48 and the lowest WBS values. Results indicated a line effect on WBS tenderness. Meat derived from the positive line was less tender compared to the meat from the negative line. Differences between the reproductive lines pertaining to the mineral and fatty acid composition were also noted. A general tendency found, was for the Positive Ewes (PE) to have the highest and the NE to have the lowest mineral concentration. Significant differences were detected between the four groups in the individual fatty acids arachidic acid (C20:0), lignoceric acid (C24:0), eicosenoic acid (C20:1n-9), linoleic acid (C18:2n-6), homo-g-linolenic acid (C20:3n-6), arachidonic acid (C20:4n-6), eicosapentaenoic acid (C20:5n-3), docosadienoic acid (C22:2n-6), docosapentaenoic acid (C22:5n-3) and docosahexaenoic acid (C22:6n-3) concentrations. The PR group had the highest total PUFA (polyunsaturated fatty acids) content (ca. 8%) and the NR the lowest PUFA content (ca. 6%). The positive group had significantly higher C18:2n-6, C20:3n-6, C20:4n-6, C20:5n-3, C22:5n-3 and C22:6n-3 concentrations in comparison with the negative line. Significant difference in the total PUFA composition was also detected between lines, with the positive line showing a higher concentration (ca. 7%) compared to the negative line (ca. 6%)

Behaviour of Merinos divergently selected for multiple rearing ability in response to external stimuli

Data of Merino sheep descended from two divergent selection lines for (H line) and against (L line) multiple rearing ability were collected in three different trials to compare response to stressful situations between lines. Trial 1: the behaviour of 138, 20-month-old sheep born in 2001 and 124, 8-month-old sheep born in 2002 were observed in an arena test. Experimental animals amounted to 111 and 101 for the H line in the respective years. Corresponding numbers for the L line were 27 and 23, respectively. Progeny born in 2001 were accustomed to handling, as the tests were performed shortly after routine performance recording. Lambs born in 2002 were unaccustomed to handling when tested. Progeny from the H line born in 2002 allowed a smaller (P < 0.01) minimum distance between them and the human operator seated between them and a group of similar aged sheep than L line contemporaries. Progeny born in the L line during 2002 defecated more (P < 0.05) frequently than H line contemporaries. No line differences were found in progeny born during 2001. Trials 2 and 3 were designed to test whether the putative line difference in stress response would impact on physical meat quality. Trial 2: a total of 103, 18-20-month-old progeny from the two selection lines were slaughtered during 2001, 2002-2003. Mean slaughter weight, dressing percentage and carcass weight of H line animals were between 5 and 13% higher (P < 0.05) than that of L line animals. There were no differences in cooking loss, drip loss and tenderness between the two lines. As pertaining to the meat colour, L line progeny had less vivid red meat, as seen by their lower (P < 0.05) a* colour reading and chroma values. The L line animals had lower (P < 0.01) initial pH values as measured in the Musculus longissimus dorsi. These results are indicative of susceptibility to acute, short-term stress. Trial 3: 20 progeny of the two selection lines were slaughtered and their muscle pH measured at 11 intervals from 45 min to 48 h post slaughter. As in Trial 1, the initial pH of the L line animals were lower (P < 0.05) than that of H line animals. An opposite tendency was observed by 48 h post slaughter. The obtained results support a hypothesis that divergent selection for ewe multiple ability resulted in adaptations to cope with unfamiliar conditions. © 2005 Elsevier B.V. All rights reserved.Articl

Terminal crossbreeding of Dorper ewes to Ile de France, Merino Landsheep and SA Mutton Merino sires: Ewe production and lamb performance

The effect of crossing Dorper ewes with Ile de France (IdF), Merino Landsheep (ML) and SA Mutton Merino (SAMM) rams in a terminal crossbreeding program was investigated during 2000 and 2001. Expressed as percentage of purebred Dorper lambs, the average birth weights of IdF cross and ML cross lambs were increased by 12 and 7%, respectively. Crossbred progeny of IdF sires were 10% heavier than purebred Dorpers at weaning. A corresponding difference in favour of ML sires amounted to 5%. IdF crossbred lambs reached slaughter weight earlier than the other cross lambs and purebred Dorper lambs. Lamb mortality prior to weaning was unaffected by the breed of the service sire, and ranged between 0.13 for lambs sired by IdF rams to 0.22 for purebred Dorper lambs. Ewe joining weight, as well as number of lambs born and weaned per ewe lambed was independent of the breed of the service sire. When weight of lamb weaned per ewe lambed was considered, there was a tendency towards higher lamb outputs in ewes that were joined to IdF and SAMM sires. Estimated repeatability derived for joining weight and the various measures of reproduction generally accorded with literature estimates. Backfat depth (taken at the 13th rib, 25 mm from the midline) indicated that purebred Dorper lambs had a thicker fat cover compared to all combinations of crossbred lambs. Corresponding sire breed differences were found between the 3rd and 4th lumbar vertebra, 25 mm from the midline. Terminal crossbreeding of Dorper ewes with IdF, ML and SAMM sires did not deleteriously affect lamb growth and survival or ewe reproduction. Terminal crossbreeding could thus be implemented in commercial Dorper flocks, without compromising productivity and/or product quality. © 2005 Elsevier B.V. All rights reserved.Articl

Sensory and objective mutton quality characteristics of SA Merino sheep selected for and against reproductive fitness

The effect of divergent selection for ewe multiple-rearing ability over a 15-year period on mutton quality was examined on the M. longissimus dorsi and M. semimembranosus of entire rams (R) and ewes (E) derived from two Merino lines. The selection of these two lines was based on maternal ranking values for multiple-rearing ability in a positive (P) and negative (N) line. In the negative line the replacements were based on the progeny of ewes that rear less than one lamb per joining or lambing opportunity (i.e. failed to lamb or lost all progeny born at least once). Progeny of ewes that reared more than one lamb per joining (i.e. reared twins at least once) were selected for the positive line. Ratings of sensory quality characteristics on the M. semimembranosus of the different genotype lines were obtained from a trained taste panel and related to data on physical and chemical characteristics. The moisture, total lipids, protein, ash, mineral concentrations and fatty acid composition of the M. semimembranosus were obtained. Physical parameters measured on the M. longissimus dorsi were: ultimate pH (pH48), drip loss, cooking loss and Warner-Bratzler shear force (WBS). The influence of selection line on the sensory characteristics of the mutton was generally negligible, with the exception of the sensory attribute of first bite, where meat derived from the positive line was rated to be less tender than that of the negative line contemporaries. Chemically, the meat derived from the four different groups differed significantly in moisture and lipid content. The Positive Ram (PR) group had the highest moisture (ca. 76%) and the lowest lipid (ca. 7%) concentrations, whereas the Negative Ewe (NE) group had the lowest moisture (ca. 70%) and the highest lipid (ca. 10%) concentration. No significant differences were detected in the proximate chemical composition between the P and N lines. The pH48 and WBS values showed significant differences between the four groups. The Negative Ram (NR) group had the highest pH48 and the lowest WBS values. Results indicated a line effect on WBS tenderness. Meat derived from the positive line was less tender compared to the meat from the negative line. Differences between the reproductive lines pertaining to the mineral and fatty acid composition were also noted. A general tendency found, was for the Positive Ewes (PE) to have the highest and the NE to have the lowest mineral concentration. Significant differences were detected between the four groups in the individual fatty acids arachidic acid (C20:0), lignoceric acid (C24:0), eicosenoic acid (C20:1n-9), linoleic acid (C18:2n-6), homo-γ-linolenic acid (C20:3n-6), arachidonic acid (C20:4n-6), eicosapentaenoic acid (C20:5n-3), docosadienoic acid (C22:2n-6), docosapentaenoic acid (C22:5n-3) and docosahexaenoic acid (C22:6n-3) concentrations. The PR group had the highest total PUFA (polyunsaturated fatty acids) content (ca. 8%) and the NR the lowest PUFA content (ca. 6%). The positive group had significantly higher C18:2n-6, C20:3n-6, C20:4n-6, C20:5n-3, C22:5n-3 and C22:6n-3 concentrations in comparison with the negative line. Significant difference in the total PUFA composition was also detected between lines, with the positive line showing a higher concentration (ca. 7%) compared to the negative line (ca. 6%).Articl

A comparison between slaughter traits and meat quality of various sheep breeds: Wool, dual-purpose and mutton

The slaughter and meat quality traits of 20-month-old wool (Merino), dual-purpose (Dohne Merino and South African Mutton Merino [SAMM]) and mutton (Dormer) type sheep were compared. Average live weights of SAMM and Dormer sheep were 23% heavier than those of Dohne Merinos which were 28% heavier than Merinos. Fat depths at the thirteenth rib and lumbar regions of Merino and Dohne Merino sheep were lower than those of SAMM and Dormer sheep. The cooking loss, drip loss and shearing value from the M. longissimus dorsi did not differ between breeds. The initial juiciness and sustained juiciness of meat from Merinos were rated significantly lower by sensory analysis. Meat from Dohne Merino was rated significantly more tender for the attribute first bite. It was demonstrated that Dormer and SAMM sheep had heavier but fatter carcasses than Merinos and Dohne Merinos, with differences in meat quality between breeds. © 2012 Elsevier Ltd

Effect of production system on the growth rate, carcass characteristics and carcass composition of Dorper lambs

An investigation on the effect of production system on growth rate, carcass characteristics and carcass composition of Dorper lambs was carried out under South African conditions. The study involved sixty lambs which were divided into two production/feeding groups (feedlot (FL) vs. free-range (FR)) at weaning. Each group consisted of 10 lambs from three gender classes (ewes, rams and castrates). The FL-lambs were fed a commercial pelleted ration while the FR-group grazed natural pastures. The production system x gender interaction indicated that male lambs (castrates and rams) grew twice as fast as ewes under FL-conditions. Gender groups were less pronounced in FR-lambs. FL-lambs produced heavier carcasses, higher dressing percentages and greater carcass fatness levels than FR-lambs. Ram lambs attained heavier slaughter weights and produced heavier carcasses than ewe lambs with castrates being intermediate. Production system did not have an effect on intramuscular lipid concentration, barring a tendency in the Biceps femoris muscle. It was concluded that the FL-diets were generally associated with better lamb growth rates, greater carcass fatness and an improved dressing percentage. Although animal performance was generally improved in the more intensive FL-system, it was also concluded that discerning consumers will likely prefer the more natural (and therefore more ethically acceptable) FR-meat