A miniature bioassay for testing the acute phytotoxicity of photosystem II herbicides on seagrass

© 2015 Wilkinson et al. Photosystem II (PSII) herbicides have been detected in nearshore tropical waters such as those of the Great Barrier Reef and may add to the pressure posed by runoff containing sediments and nutrients to threatened seagrass habitats. There is a growing number of studies into the potential effects of herbicides on seagrass, generally using large experimental set-ups with potted plants. Here we describe the successful development of an acute 12-well plate phytotoxicity assay for the PSII herbicide Diuron using isolated Halophila ovalis leaves. Fluorescence images demonstrated Diuron affected the entire leaf surface evenly and responses were not influenced by isolating leaves from the plant. The optimum exposure duration was 24 h, by which time the inhibition of effective quantum yield of PSII (ΔF/Fm′) was highest and no deterioration of photosystems was evident in control leaves. The inhibition of ΔF/Fm′ by Diuron in isolated H. ovalis leaves was identical to both potted and hydroponically grown plants (with leaves remaining attached to rhizomes), indicating similar reductions in photosynthetic activity in these acute well-plate assays. The sensitivity of the assay was not influenced by irradiance (range tested 40 to 400 μmol photons m-2 s-1). High irradiance, however, caused photo-oxidative stress in H. ovalis and this generally impacted in an additive or sub-additive way with Diuron to damage PSII. The bioassay using isolated leaves is more rapid, uses far less biological material and does not rely on specialised aquarium facilities in comparison with assays using potted plants. The development and validation of this sensitive bioassay will be useful to reliably screen and monitor the phytotoxicity of existing and emerging PSII herbicides and contribute to risk assessments and water quality guideline development in the future

The latitudinal temperature gradient and its climate dependence as inferred from foraminiferal δ18O over the past 95 million years

The latitudinal temperature gradient is a fundamental state parameter of the climate system tied to the dynamics of heat transport and radiative transfer. Thus, it is a primary target for temperature proxy reconstructions and global climate models. However, reconstructing the latitudinal temperature gradient in past climates remains challenging due to the scarcity of appropriate proxy records and large proxy–model disagreements. Here, we develop methods leveraging an extensive compilation of planktonic foraminifera δ18O to reconstruct a continuous record of the latitudinal sea-surface temperature (SST) gradient over the last 95 million years (My). We find that latitudinal SST gradients ranged from 26.5 to 15.3 °C over a mean global SST range of 15.3 to 32.5 °C, with the highest gradients during the coldest intervals of time. From this relationship, we calculate a polar amplification factor (PAF; the ratio of change in >60° S SST to change in global mean SST) of 1.44 ± 0.15. Our results are closer to model predictions than previous proxy-based estimates, primarily because δ18O-based high-latitude SST estimates more closely track benthic temperatures, yielding higher gradients. The consistent covariance of δ18O values in low- and high-latitude planktonic foraminifera and in benthic foraminifera, across numerous climate states, suggests a fundamental constraint on multiple aspects of the climate system, linking deep-sea temperatures, the latitudinal SST gradient, and global mean SSTs across large changes in atmospheric CO2, continental configuration, oceanic gateways, and the extent of continental ice sheets. This implies an important underlying, internally driven predictability of the climate system in vastly different background states

Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations

Micronutrient malnutrition afflicts over three billion peopleworldwide and the numbers are continuously increasing. Developing genetically micronutrientenriched cereals, which are the predominant source of human dietary, is essential to alleviate malnutrition worldwide. Wheat chromosome 6B derived from wild emmerwheat [Triticum turgidum ssp. dicoccoides (Körn.) Thell] was previously reported to be a source for high Zn concentration in the grain. In the present study, recombinant chromosome substitution lines (RSLs), previously constructed for genetic and physical maps of Gpc-B1 (a 250-kb locus affecting grain protein concentration), were used to identify the effects of the Gpc-B1 locus on grain micronutrient concentrations. RSLs carrying the Gpc-B1 allele of T. dicoccoides accumulated on average 12% higher concentration of Zn, 18% higher concentration of Fe, 29% higher concentration of Mn and 38% higher concentration of protein in the grain as compared with RSLs carrying the allele from cultivated wheat (Triticum durum). Furthermore, the high grain Zn, Fe and Mn concentrations were consistently expressed in five different environments with an absence of genotype by environment interaction. The results obtained in the present study also confirmed the previously reported effect of the wild-type allele of Gpc-B1 on earlier senescence of flag leaves. We suggest that the Gpc-B1 locus is involved in more efficient remobilization of protein, zinc, iron and manganese from leaves to the grains, in addition to its effect on earlier senescence of the green tissues

Evolution of anisotropic turbulence in the fast and slow solar wind: Theory and Solar Orbiter measurements

Aims: Solar Orbiter (SolO) was launched on February 9, 2020, allowing us to study the nature of turbulence in the inner heliopshere. We investigate the evolution of anisotropic turbulence in the fast and slow solar wind in the inner heliosphere using the nearly incompressible magnetohydrodynamic (NI MHD) turbulence model and SolO measurements. Methods: We calculated the two dimensional (2D) and the slab variances of the energy in forward and backward propagating modes, the fluctuating magnetic energy, the fluctuating kinetic energy, the normalized residual energy, and the normalized cross-helicity as a function of the angle between the mean solar wind speed and the mean magnetic field (θUB), and as a function of the heliocentric distance using SolO measurements. We compared the observed results and the theoretical results of the NI MHD turbulence model as a function of the heliocentric distance. Results: The results show that the ratio of 2D energy and slab energy of forward and backward propagating modes, magnetic field fluctuations, and kinetic energy fluctuations increases as the angle between the mean solar wind flow and the mean magnetic field increases from θUB = 0 ◦ to approximately θUB = 90◦ and then decreases as θUB → 180◦ . We find that solar wind turbulence is a superposition of the dominant 2D component and a minority slab component as a function of the heliocentric distance. We find excellent agreement between the theoretical results and observed results as a function of the heliocentric distance

Designing theoretically-informed implementation interventions

Clinical and health services research is continually producing new findings that may contribute to effective and efficient patient care. However, the transfer of research findings into practice is unpredictable and can be a slow and haphazard process. Ideally, the choice of implementation strategies would be based upon evidence from randomised controlled trials or systematic reviews of a given implementation strategy. Unfortunately, reviews of implementation strategies consistently report effectiveness some, but not all of the time; possible causes of this variation are seldom reported or measured by the investigators in the original studies. Thus, any attempts to extrapolate from study settings to the real world are hampered by a lack of understanding of the effects of key elements of individuals, interventions, and the settings in which they were trialled. The explicit use of theory offers a way of addressing these issues and has a number of advantages, such as providing: a generalisable framework within which to represent the dimensions that implementation studies address, a process by which to inform the development and delivery of interventions, a guide when evaluating, and a way to allow for an exploration of potential causal mechanisms. However, the use of theory in designing implementation interventions is methodologically challenging for a number of reasons, including choosing between theories and faithfully translating theoretical constructs into interventions. The explicit use of theory offers potential advantages in terms of facilitating a better understanding of the generalisability and replicability of implementation interventions. However, this is a relatively unexplored methodological area

Comparison of self-reported health & healthcare utilisation between asylum seekers and refugees: an observational study

<p>Abstract</p> <p>Background</p> <p>Adult refugees and asylum seekers living in Western countries experience a high prevalence of mental health problems, especially post traumatic stress disorder (PTSD), depression and anxiety. This study compares and contrasts the prevalence of health problems, and potential risk factors as well as the utilisation of health services by asylum seekers and refugees in the Irish context.</p> <p>Methods</p> <p>Cross sectional study using validated self reported health status questionnaires of adult asylum seekers (n = 60) and refugees (n = 28) from 30 countries, living in Ireland. Outcome measures included: general health status (SF-36), presence of PTSD symptoms and anxiety/depression symptoms. Data on chronic conditions and pre or post migration stressors are also reported. The two groups are compared for utilisation of the health care system and the use of over the counter medications.</p> <p>Results</p> <p>Asylum seekers were significantly more likely than refugees to report symptoms of PTSD (OR 6.3, 95% CI: 2.2–17.9) and depression/anxiety (OR 5.8, 95% CI: 2.2–15.4), while no significant difference was found in self-reported general health. When adjusted by multivariable regression, the presence of more than one chronic disease (OR 4.0, 95%CI: 1.3–12.7; OR 3.4, 95% CI: 1.2–10.1), high levels of pre migration stressors (OR 3.6, 95% CI: 1.1–11.9; OR 3.3, 95% CI: 1.0–10.4) or post migration stressors (OR 17.3, 95% CI: 4.9–60.8; OR 3.9, 95% CI: 1.2–12.3) were independent predictors of self reported PTSD or depression/anxiety symptoms respectively, however, residence status was no longer significantly associated with PTSD or depression/anxiety. Residence status may act as a marker for other explanatory variables; our results show it has a strong relationship with post migration stressors (χ²= 19.74, df = 1, P < 0.001).</p> <p>In terms of health care utilisation, asylum seekers use GP services more often than refugees, while no significant difference was found between these groups for use of dentists, medication, hospitalisation or mental health services.</p> <p>Conclusion</p> <p>Asylum seekers have a higher level of self reported PTSD and depression/anxiety symptoms compared to refugees. However, residence status appears to act as a marker for post migration stressors. Compared to refugees, asylum seekers utilise GP services more often, but not mental health services.</p

Multi-spacecraft study of the solar wind at solar minimum: Dependence on latitude and transient outflows

Context: The recent launches of Parker Solar Probe, Solar Orbiter (SO), and BepiColombo, along with several older spacecraft, have provided the opportunity to study the solar wind at multiple latitudes and distances from the Sun simultaneously. Aims: We take advantage of this unique spacecraft constellation, along with low solar activity across two solar rotations between May and July 2020, to investigate how the solar wind structure, including the heliospheric current sheet (HCS), varies with latitude. Methods: We visualise the sector structure of the inner heliosphere by ballistically mapping the polarity and solar wind speed from several spacecraft onto the Sun’s source surface. We then assess the HCS morphology and orientation with the in situ data and compare this with a predicted HCS shape. Results: We resolve ripples in the HCS on scales of a few degrees in longitude and latitude, finding that the local orientations of sector boundaries were broadly consistent with the shape of the HCS but were steepened with respect to a modelled HCS at the Sun. We investigate how several CIRs varied with latitude, finding evidence for the compression region affecting slow solar wind outside the latitude extent of the faster stream. We also identified several transient structures associated with HCS crossings and speculate that one such transient may have disrupted the local HCS orientation up to five days after its passage. Conclusions: We have shown that the solar wind structure varies significantly with latitude, with this constellation providing context for solar wind measurements that would not be possible with a single spacecraft. These measurements provide an accurate representation of the solar wind within ±10° latitude, which could be used as a more rigorous constraint on solar wind models and space weather predictions. In the future, this range of latitudes will increase as SO’s orbit becomes more inclined

An Open-System Quantum Simulator with Trapped Ions

The control of quantum systems is of fundamental scientific interest and promises powerful applications and technologies. Impressive progress has been achieved in isolating the systems from the environment and coherently controlling their dynamics, as demonstrated by the creation and manipulation of entanglement in various physical systems. However, for open quantum systems, engineering the dynamics of many particles by a controlled coupling to an environment remains largely unexplored. Here we report the first realization of a toolbox for simulating an open quantum system with up to five qubits. Using a quantum computing architecture with trapped ions, we combine multi-qubit gates with optical pumping to implement coherent operations and dissipative processes. We illustrate this engineering by the dissipative preparation of entangled states, the simulation of coherent many-body spin interactions and the quantum non-demolition measurement of multi-qubit observables. By adding controlled dissipation to coherent operations, this work offers novel prospects for open-system quantum simulation and computation.Comment: Pre-review submission to Nature. For an updated and final version see publication. Manuscript + Supplementary Informatio