Using in situ technique to estimate nutrient availability of six barley varieties for ruminants

Non-Peer ReviewedBarley grains are as a main source of feed for ruminants in Canada. Although barley varieties have similar chemical composition, they exhibit different rumen degradation characteristics and nutrient availability. The objective of this study was to estimate nutrient availability of the six barley varieties using nylon bags incubated for 0, 2, 4, 8, 12, 24 and 48 h in the rumen of dairy cow and determine the rumen degradation parameters of DM, CP and starch according to the first order degradation kinetics equation. The results indicated that the barley varieties showed different degradation rate (Kd) of DM, CP and starch during incubation period. CDC Trey was the highest (P<0.05) in effective degradability of DM (498.04g/kg) while AC Metcalfe showed the highest (P<0.05) effective degradability of CP (63.06g/kg) and starch (344.82g/kg) compared with other barley varieties. CDC Helgason had the largest size of rumen undegradable fraction of DM (640.21g/kg), CP (83.85g/kg, NRC 2001 model) and starch (321.85g/kg). Feeds degradation features from this study involved nutrients utilization in ruminant digestive systems. Further study to investigate and compare the structural/chemical make-up characteristics between barley varieties may provide more information as to why barley varieties exhibit different biodegradation behaviors

Using synchrotron-based FTIR microspectroscopy (SFTIRM) to reveal the differences of endosperm structural and chemical make-up among six barley varieties

Non-Peer ReviewedBarley grains are widely used for malting and feeding purposes in Canada. Although barley varieties have similar chemical composition, they exhibit different rumen degradation characteristics and nutrient availability. The objectives of this study was to determine structural make-up features and identify the structural differences in chemical functional groups in endosperm tissue among the six barley varieties using ultra-spatially resolved synchrotron-based FTIR microspectroscopy (SFTIRM). The results indicated that the barley varieties showed significant differences in terms of peak area intensities and the peak ratios of the amide I (1650 cm-1) and amide II (1550 cm-1), cellulosic compounds (ca. 1240 cm-1), and non-structural carbohydrates (NSC, starch) peak (1025 cm-1). The synchrotron-based FTIR spectroscopic information associated with structural and chemical make-up characteristics of barley grains may provide more information as to why barley varieties exhibit different biodegradation behaviors

Characteristics and utilization of canola seed fractions in ruminant feeds a processing and value added research

Non-Peer Reviewe

Hulless barley (Hordeum vulgare l.) with altered starch traits: rumen degradation kinetics

Non-Peer ReviewedThe objective of this study was to determine the effect of new hulless barley lines (zero-amylose waxy, CDC Fibar; 5%-amylose waxy, CDC Rattan; normal-amylose, CDC McGwire and high-amylose, HB08302) on rumen degradation kinetics and in vitro intestinal nutrient digestion with CDC Copeland included as a hulled control. Three dry Holstein cows fitted with rumen cannula were used for measuring rumen degradation of barley varieties. The estimation of intestinal digestion was determined by a modification of the three-step in vitro procedure described by Calsamiglia and Stern with duplicate of each in situ residue. Among the hulless barley lines, CDC Rattan was greater (P<0.05) in effectively degradable starch (EDST: 461 g/kg DM) but reduced (P<0.05) in rumen undegradable protein (RUP: 55g/kg DM) while CDC Fibar was greater (P<0.05) in effectively degradable crude protein (EDCP: 90 g/kg DM) and total digestible protein (TDP: 147 g/kg DM) than other hulless barley lines. CDC McGwire showed greater (P<0.05) in total digestible bypass starch (TDST: 590 g/kg DM), effectively degradable CHO (EDCHO: 581 g/kg DM) and total digestible CHO (TDCHO: 600 g/kg DM) than other hulless barley lines but no significant difference compared to CDC Copeland (P>0.05). HB08302 was greater (P<0.05) in rumen bypass starch (BST: 218 g/kg DM vs. 146 g/kg DM and 155 g/kg DM) and effective degradable NDF (EDNDF: 74 g/kg DM vs. 49 g/kg DM and 52 g/kg DM) than CDC Fibar and CDC Rattan. HB08302 was also greater in total digestible NDF (TDNDF: 93 g/kg DM vs. 62 g/kg DM and 67 g/kg DM) and intestinal digestible rumen bypass starch (IDBST: 180 g/kg DM vs. 122 g/kg DM and 130 g/kg DM) compared to CDC Fibar and CDC McGwire. HB08302 also showed greater (P<0.05) in bypass CHO (BCHO: 179 g/kg DM) and intestinal digestible bypass CHO (IDBCHO: 31 g/kg DM) than other hulless barley lines. Amylose and amylopectin were significantly correlated (P<0.001) to EDCP (r=-0.71, r=0.64) and TDP (r=-0.85; r=0.77), while amylose, ratio of amylose and amylopectin were positively correlated (P<0.01) to EDNDF, TDNDF, BST, IDBST and TDCHO (P<0.05). BCP, IDP, TDP, BNDF were positively correlated to beta-glucan levels (P<0.001) while EDST, TDST, EDCHO and TDCHO were negatively correlated to beta-glucan levels in hulless barley lines (P<0.01). in conclusion hulless barley lines with altered carbohydrate traits have the potential to increase rumen and intestinal nutrient availability to ruminants. Altered beta-glucan levels had a greater effect on rumen carbohydrates and crude protein degradation than altered starch traits

Actinomyces species: A Danish Survey on Human Infections and Microbiological Characteristics

This study compared phenotypic and genotypic identification of Actinomyces strains, tested susceptibility to antibiotics and evaluated their clinical importance. Thirty-four Actinomyces strains were examined; sixteen type strains, and 18 clinical strains from different hospitals in Denmark from the period 2003-2005. Partial 16S rDNA sequencing using a stretch of 526 bases was used for genotypic identification. Susceptibility testing was done by E-test. The antibiotics examined were: benzylpenicillin, piperacillin with tazobactam, ceftriaxone, meropenem, erythromycin, clindamycin, linezolid, moxifloxacin, tetracycline and tigecycline. Clinical parameters were obtained by reviewing patient records. There was poor agreement between the phenotypic and genotypic identification. Phenotypic tests were helpful in identifying strains closely related by DNA sequences. The strains were sensitive to the examined antibiotics except for moxifloxacin to which most strains were resistant, and a few strains were resistant to meropenem and tetracycline. The clinical strains were from many different types of infections and locations. None of the patients was described as having typical actinomycetic lesions, and an apparently good outcome was obtained with different treatment regimens

Cloud-Based Speech Technology for Assistive Technology Applications (CloudCAST)

The CloudCAST platform provides a series of speech recognition services that can be integrated into assistive technology applications. The platform and the services provided by the public API are described. Several exemplar applications have been developed to demonstrate the platform to potential developers and users

An innovative speech-based user interface for smarthomes and IoT solutions to help people with speech and motor disabilities

A better use of the increasing functional capabilities of home automation systems and Internet of Things (IoT) devices to support the needs of users with disability, is the subject of a research project currently conducted by Area Ausili (Assistive Technology Area), a department of Polo Tecnologico Regionale Corte Roncati of the Local Health Trust of Bologna (Italy), in collaboration with AIAS Ausilioteca Assistive Technology (AT) Team. The main aim of the project is to develop experimental low cost systems for environmental control through simplified and accessible user interfaces. Many of the activities are focused on automatic speech recognition and are developed in the framework of the CloudCAST project. In this paper we report on the first technical achievements of the project and discuss future possible developments and applications within and outside CloudCAST

Alpha oscillations related to self-other integration and distinction during live orchestral performance: a naturalistic case study

Ensemble music performance requires musicians to achieve precise interpersonal coordination while maintaining autonomous control over their own actions. To do so, musicians dynamically shift between integrating other performers’ actions into their own action plans and maintaining a distinction between their own and others’ actions. Research in laboratory settings has shown that this dynamic process of self-other integration and distinction is indexed by sensorimotor alpha oscillations. The purpose of the current descriptive case study was to examine oscillations related to self-other integration and distinction in a naturalistic performance context. We measured alpha activity from four violinists during a concert hall performance of a 60-musician orchestra. We selected a musical piece from the orchestra’s repertoire and, before analyzing alpha activity, performed a score analysis to divide the piece into sections that were expected to strongly promote self-other integration and distinction. In line with previous laboratory findings, performers showed suppressed and enhanced alpha activity during musical sections that promoted self-other integration and distinction, respectively. The current study thus provides preliminary evidence that findings from carefully controlled laboratory experiments generalize to complex real-world performance. Its findings also suggest directions for future research and potential applications of interest to musicians, music educators, and music therapists