Etude preliminaire de l'association isoniazide-epiroprim dans un modele murin de tuberculose

International audienceThe purpose of this study regarding isoniazid-epiroprim's association applied to antituberculosis chemotherapy, carried through murine model, initiated into Institut Pasteur of Côte d'Ivoire and worked out at Institut Pasteur of Paris was to evaluate the epiroprim's effect alone and associated with isoniazid on Mycobacterium tuberculosis. Sixteen mouses (lineage C57Bl/6) were inoculated by venous way with 10(5) viable bacillus (strain H37Rv) suspended in 500 microliters sterile physiological aqueous solution and were shared out into 4 sets. Fifteen days later the sets have been submitted or not to a daily treatment by gavage during three weeks (epiroprim, isoniazid, isoniazid plus epiroprim). The mouses were euthanasied, spleen and lung were removed from each animal. The titres of determined bacillus into those organs prove that isoniazid and epiroprim associated seem more efficacious than the isoniazid monotherapy for mouses pulmonary tuberculosis. Bacillus obtained are sensitive to isoniazid.Cette étude de chimiothérapie antituberculeuse expérimentale de l'association isoniazide-épiroprim dans un modèle murin initiée à l'Institut Pasteur de Côte d'Ivoire et réalisée à l'Institut Pasteur à Paris avait pour objectif d'évaluer l'effet de l'épiroprim, un inhibiteur de la dihydrofolate reductase seul et en association avec l'isoniazide sur Mycobacterium tuberculosis. 16 souris (lignée C57Bl/6) recevant chacune par voie veineuse un inoculum contenant 10 5 bacilles viables (souche H37RV) en suspension dans 500 µl de soluté physiologique stérile ont été réparties en quatre lots. 15 jours après l'inoculation, les lots ont été pendant trois semaines soumis ou non à un traitement journalier (épiroprim, isoniazide, isoniazide épiroprim) par gavage. Au terme du traitement, les souris ont été euthanasiées, la rate et le poumon prélevés chez chaque animal. Les titres de bacilles déterminés dans ces organes montrent que l'association isoniazide-épiroprim semble plus efficace que la monothérapie à l'isoniazide pour le traitement de la tuberculose pulmonaire de la souris. Les bacilles obtenus à partir de ces organes sont sensibles à l'isoniazide

Development of the rumen digestive functions in lambs placed in a sterile isolator a few days after birth

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Push–pull organic dyes and dye-catalyst assembly featuring a benzothiadiazole unit for photoelectrochemical hydrogen production

International audienceIn this work, we report the design and the preparation of two new dyes and a molecular dyad for the photoelectrochemical hydrogen production from water in a dye-sensitized photoelectrochemical cell (DSPEC). We designed dyes that include a benzothiadiazole (BTD) and an indacenodithiophene (IDT) units, and we obtained a new molecular dyad by covalent coupling with the cobalt diimine–dioxime catalyst. The introduction of the benzothiadiazole core in the structure improves the absorption properties and leads to an extension of the spectrum in the visible range up to 650 nm. The photoelectrochemical properties of the new dyad were evaluated on pristine and lithium-doped NiO electrodes. We demonstrate that increasing the light harvesting efficiency of the dyad by introducing a IDT–BTD chromophore is clearly beneficial for the photoelectrochemical activity. We also demonstrate that lithium doping of NiO, which improves the electronic conductivity of the mesoporous film, leads to a significant increase in performance, in terms of TON and F.E., more than doubled with our new dyad. This BTD-based molecular system outperforms the results of previously reported dyads using the same catalys