Pixel super-resolution in optical time-stretch microscopy using acousto-optic deflector

Session - Biosensing and Bio-Manipulation Techniques II (BW2A): paper BW2A.7Bio-Optics: Design and Application (BODA)We present experimental demonstration of pixel super-resolution time-stretch imaging by high-speed agile-beam-steering with the use of synchronized acousto-optic deflector--enabling high-resolution imaging rate of 1MHz whereas relaxing the stringent requirement on extreme data acquisition. © 2015 OSApostprin

Pixel super-resolution of time-stretch imaging by an equivalent-time sampling concept

Optical time-stretch imaging entails a stringent requirement of state-of-the-art high-speed data acquisition unit in order to preserve high image resolution at an ultrahigh frame rate-hampering the widespread application of such technology. We here propose a pixel super-resolution (pixel SR) technique tailored for time-stretch imaging that can relax the sampling rate requirement. It harnesses a concept of equivalent-time sampling, which effectively introduces sub-pixel shifts between frames. It involves no active opto-mechanical subpixel-shift control and any additional hardware. We present the system design rules and a proof-of-principle experiment which restores high-resolution images at a relaxed sampling rate of 5 GSa=s. © 2016 SPIE.published_or_final_versio

Pixel super-resolution in serial time-encoded amplified microscopy (STEAM)

Paper no. CTu3J.4We propose pixel super-resolution serial time-encoded amplified microscopy (STEAM) for achieves high speed and high-resolution imaging - relaxing the stringent requirement on the digitizer bandwidth while preserving the ultrahigh frame-rate (>MHz). © 2012 OSA.published_or_final_versio

Depth enhancement of optical scanning holography with a spiral phase plate

Poster Session (DW2A): no. DW2A.3A spiral phase plate is applied to the optical scanning holography system to improve the depth resolution of the reconstruction, the simulation results show that the depth interval can be resolved at a 0.4 mm with only a single hologram. © 2015 OSApostprin

Speed-dependent resolution analysis of ultrafast laser-scanning fluorescence microscopy

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Brief advice and active referral for smoking cessation services among community smokers: a study protocol for randomized controlled trial

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Revisit laser scanning fluorescence microscopy performance under fluorescence-lifetime-limited regime

Continuing desire for higher-speed laser scanning fluorescence microscopy (LSFM) and progressive advancement in ultrafast and sensitive photodetectors might imply that our conventional understanding of LSFM is not adequate when approaching to the intrinsic speed limit - fluorescence lifetime. In this regard, we here revisit the theoretical framework of LSFM and evaluate its general performance in lifetime-limited and noise-limited regimes. Our model suggests that there still exists an order-of-magnitude gap between the current LSFM speed and the intrinsic limit. An imaging frame rate of > 100 kHz could be viable with the emerging laser-scanning techniques using ultrafast wavelength-swept sources, or optical time-stretch. © 2014 SPIE

Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm

Quantitative phase imaging (QPI) has been proven to be a powerful tool for label-free characterization of biological specimens. However, the imaging speed, largely limited by the image sensor technology, impedes its utility in applications where high-throughput screening and efficient big-data analysis are mandated. We here demonstrate interferometric time-stretch (iTS) microscopy for delivering ultrafast quantitative phase cellular and tissue imaging at an imaging line-scan rate >20 MHz-orders-of-magnitude faster than conventional QPI. Enabling an efficient time-stretch operation in the 1-mum wavelength window, we present an iTS microscope system for practical ultrafast QPI of fixed cells and tissue sections, as well as ultrafast flowing cells (at a flow speed of up to 8 ms). To the best of our knowledge, this is the first time that time-stretch imaging could reveal quantitative morphological information of cells and tissues with nanometer precision. As many parameters can be further extracted from the phase and can serve as the intrinsic biomarkers for disease diagnosis, iTS microscopy could find its niche in high-throughput and high-content cellular assays (e.g., imaging flow cytometry) as well as tissue refractometric imaging (e.g., whole-slide imaging for digital pathology).published_or_final_versio

Optical time-stretch microscopy enabled by free-space angular-chirp-enhanced delay

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Study of Zγ events and limits on anomalous ZZγ and Zγγ couplings in pp̄ collisions at s=1.96TeV

We present a measurement of the Zγ production cross section and limits on anomalous ZZγ and Zγγ couplings for form-factor scales of Λ=750 and 1000 GeV. The measurement is based on 138 (152) candidates in the eeγ (μμγ) final state using 320(290)pb-1 of pp̄ collisions at s=1.96TeV. The 95% C.L. limits on real and imaginary parts of individual anomalous couplings are |h10,30Z|<0.23, |h20,40Z|<0.020, |h10,30γ|<0.23, and |h20,40γ|<0.019 for Λ=1000GeV. © 2005 The American Physical Society