Human papillomavirus detection and p16 methylation pattern in a case of esophageal papilloma

Esophageal cancer is a prevalent cancer worldwide. Some studies have reported the possible etiology of human papillomavirus (HPV) in benign and malignant papillomas of the esophagus but the conclusions are controversial. In the present study, we investigated an esophageal papilloma from a 30-year-old male patient presenting aphasia. HPV DNA was detected by generic PCR using MY09/11 primers, and restriction fragment length polymorphism revealed the presence of HPV54, usually associated with benign genital lesions. Hypermethylation of the pINK4A gene was also investigated due to its relation to malignant transformation, but no modification was detected in the host gene. Except for an incipient reflux, no risk factors such as cigarette smoking, alcohol abuse or an infected sexual partner were recorded. Since esophageal lesions may have a malignant potential, HPV detection and typing are useful tools for patient follow-up

Human papillomavirus infection among sexual partners attending a Sexually Transmitted Disease Clinic in Rio de Janeiro, Brazil

Cervical cancer is a major source of illness and death among women worldwide and genital infection with oncogenic human papillomavirus (HPV) its principal cause. There is evidence of the influence of the male factor in the development of cervical neoplasia. Nevertheless, the pathogenic processes of HPV in men are still poorly understood. It has been observed that different HPV types can be found among couples. The objective of the present study was to investigate HPV infections in female patients (n = 60 females/group) as well as in their sexual partners and to identify the concordance of HPV genotypes among them. By using the polymerase chain reaction, we detected a 95% prevalence of HPV DNA in women with cervical intraepithelial neoplasia (CIN) compared to 18.3% in women with normal cervical epithelium, with a statistically significant difference (P < 0.001). The HPV DNA prevalence was 50% in male partners of women with CIN and 16.6% in partners of healthy women. In the control group (healthy women), only 9 couples were simultaneously infected with HPV, and only 22.2% of them had the same virus type, showing a weak agreement rate (kappa index = 0.2). Finally, we observed that HPV DNA was present in both partners in 30 couples if the women had CIN, and among them, 53.3% shared the same HPV type, showing moderate agreement, with a kappa index of 0.5. This finding supports the idea of circulation and recirculation of HPV among couples, perpetuating HPV in the sexually active population, rather than true recurrences of latent infections

Human papillomavirus infection in Rio de Janeiro, Brazil: a retrospective study

There is considerable data to support a central role for human papillomavirus (HPV) in the etiology of cervical cancer. More than a 100 HPV types have been described, and 40 have been isolated from benign and malignant genital lesions. Consequently, there is strong motivation to evaluate HPV testing for cervical cancer screening. Few studies concerning the natural history of HPV infection have been conducted in the state of Rio de Janeiro. We determined the prevalence of HPV types in female genital lesions by using Hybrid Capture Assay (HCA) and we retrospectively analyzed the course of HPV infection. Our sample included 788 women attended at Laboratórios Sérgio Franco. The average age of the participants was 29.6 years. HPV prevalence and cytological diagnosis were determined. The overall prevalence of HPV DNA in the study group was 50.1% (395/788), ranging from 25% (NORMAL) to 100% in high-grade intraepithelial lesions (HSIL). High risk HPV was found in 12% inflammatory, 58.3% HPV, 63.2% LSIL and 100% HSIL. A retrospective analysis of 78 patients showed that 22 presented persistent lesions, 2 had progressive lesions, 4 had regressive lesions, 13 showed latent infections, 18 were transiently infected and 19 were submitted to curative treatment. No cases of cancer were registered in this population, which can afford private medical care and regular follow-up exams. We suggest that HCA be used in specific cases involving persistent and recurrent lesions

Human papillomavirus infection in Rio de Janeiro, Brazil: a retrospective study

There is considerable data to support a central role for human papillomavirus (HPV) in the etiology of cervical cancer. More than a 100 HPV types have been described, and 40 have been isolated from benign and malignant genital lesions. Consequently, there is strong motivation to evaluate HPV testing for cervical cancer screening. Few studies concerning the natural history of HPV infection have been conducted in the state of Rio de Janeiro. We determined the prevalence of HPV types in female genital lesions by using Hybrid Capture Assay (HCA) and we retrospectively analyzed the course of HPV infection. Our sample included 788 women attended at Laboratórios Sérgio Franco. The average age of the participants was 29.6 years. HPV prevalence and cytological diagnosis were determined. The overall prevalence of HPV DNA in the study group was 50.1% (395/788), ranging from 25% (NORMAL) to 100% in high-grade intraepithelial lesions (HSIL). High risk HPV was found in 12% inflammatory, 58.3% HPV, 63.2% LSIL and 100% HSIL. A retrospective analysis of 78 patients showed that 22 presented persistent lesions, 2 had progressive lesions, 4 had regressive lesions, 13 showed latent infections, 18 were transiently infected and 19 were submitted to curative treatment. No cases of cancer were registered in this population, which can afford private medical care and regular follow-up exams. We suggest that HCA be used in specific cases involving persistent and recurrent lesions

Is There Increase Of Std During Carnival? Time Series Of Diagnoses In A Std Clinic [há Aumento De Dst No Carnaval? Série Temporal De Diagnósticos Em Uma Clínica De Dst]

Objective. Sexually Transmitted Diseases (STD) are often the cause for search for medical assistance. Media campaigns on STD/Aids presume that exposure to sexual risk practices during Carnival is greater than in other periods of the year. The objective here is to study the temporal distribution of first consultations in a STD clinic, from January, 1993, to December, 2005, to verify whether there is a seasonal increase of STD after Carnival. Methods. Two thousand, six hundred and fifty six (2,656) records of patients who had received a diagnosis of gonorrhea, syphilisor trichomoniasis were selected. These patients at the time had looked for assistance at the STD Division of the Universidade Federal Fluminense, in Niterói, State of Rio de Janeiro, Brazil. The standardized mean of the number of consultations during those 13 years, month by month, and year by year was observed in the statistical analysis and also smoothed series by the Lowess method and by the deterministic method of the variable mean was considered. Results. July and August showed an increased number of diagnoses for gonorrhea and syphilis, while in June and July there was a concentration of trichomoniasis diagnoses. Gonorrhea presented its highest value in May, tending to a reduction until August. A constant number of syphilis diagnoses was noted between May and August, with a decreased number in January and February, reaching a peak in November. The seasonal behavior of trichomoniasis showed the greatest number of diagnoses in July, with a consistent reduction tendency until December, and an increase from January on. Conclusion. The Carnival period has no influence on the increase of gonorrhea, syphilis and trichomoniasis diagnoses in patients observed in a STD clinic in Niterói, Rio de Janeiro.564420427Passos, M.R.L., (2005) Deessetologia - DST-5, , 5 a ed. Rio de Janeiro: Cultura Médica(1999) Manual De Controle De Doenças Sexualmente Transmissíveis, , Brasil. Ministério da Saúde, 3 a ed. Brasília (DF): PN DST/AIDSMinistério da Saúde(2006) Manual De Controle De Doenças Sexualmente Transmissíveis, , Brasil. Ministério da Saúde, 4 a ed. Brasília (DF): PN DST/AIDSMinistério da Saúdehttp://www.aids.gov.br/data/pages, Brasil. Ministério da Saúde, PN-DST/AIDS, 2003, [citado 7 dez 2007]. Disponível emAdaora, A., Adimora, H.H., Sexually transmitted diseases (1994) Epidemiology of Sexually Transmitted Diseases, p. 436. , 2nd ed. New York: Mc Grawhil, Chapter 1Eng, T.R., Butler, W.T., (1997) The Hidden Epidemic: Confronting Sexually Transmitted Diseases, , Washington (DC): National Academy Press(2006) Disease Control Priorities in Developing Countries, , Jamison DT, Breman JG, Measham AR, Alleyne G, Claeson M, Evans DB, et al. editors, Washington (DC): World Bank PublicationsPassos, M.R.L., (2006) Deessetologia No Bolso. O Que Deve Saber Um Profissional Que Atende DST, , 2 a.ed. Piraí: RQVHolmes, K.K., (1999) Sexually Transmitted Diseases, , 3nd ed. New York: Mc GrawhillPassos, M.R.L., Ocorrênçia de doenças sexualmente transmissíveis antes e depois do carnaval no Rio de Janeiro (2002) DST J Bras Doenças Sex Transm, 14, pp. 38-42Araújo, H., (2003) Carnaval: Seis Milênios De Historia, , Rio de Janeiro: GriphusLima, C., Evoe: História do carnaval: Das tradições mitológicas ao trio elétrico (2003) Recife: Mart ShoppingDST Em Números, , http://www.aids.gov.br/data/pages/LUMISD1F318A3ITEMID55D35F0070A24175BB4DF9DD1832A658PTBRIE.htm, Ministério da Saúde, [citado 22 maio 2007]. Disponível emMensagem Recebida Por, , [email protected], Campanhas do carnaval ASCOM-PNDST/AIDS. 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