Análise energética do novo sistema de produção de maracujá amarelo na região de Marília-SP

Objetivou-se avaliar indicadores energéticos de um novo sistema de produção do maracujá amarelo na região de Marília-SP. Foram analisadas as "entradas" de energia de origem biológica, fóssil e industrial e a "saída" energética na forma de frutos produzidos por unidade de área, bem como cinco indicadores energéticos. A entrada energética totalizou 155.810,13 MJ/ha. Os adubos químicos e defensivos responderam por 71% e 24% da energia indireta industrial, respectivamente. A atividade gerou, por ciclo/ha, 587.700,00MJ, sendo a produtividade cultural 0,19MJ kg-1. A eficiência cultural foi de 3,77. A energia cultural líquida totalizou 431.889,87 MJ/ha. A eficiência energética (4.17) e o balanço energético (550.312,91MJ ha-1) foram positivos. Concluiu-se que o consumo de energia direta de origem fóssil, mesmo que significativo, ainda permite a produção do fruto de maneira ambientalmente sustentável. Os adubos químicos e defensivos foram os itens mais representativos da energia indireta industrial, em decorrência da intensificação da adubação como forma de prevenção de doenças

Heat And Phosphate Starvation Effects On The Proteome, Morphology And Chemical Composition Of The Biomining Bacteria Acidithiobacillus Ferrooxidans

Acidithiobacillus ferrooxidans is a Gram negative, acidophilic, chemolithoautotrophic bacterium that plays an important role in metal bioleaching. During bioleaching, the cells are subjected to changes in the growth temperature and nutrients starvation. The aim of this study was to gather information about the response of the A. ferrooxidans Brazilian strain LR to K2HPO4 starvation and heat stress through investigation of cellular morphology, chemical composition and differential proteome. The scanning electron microscopic results showed that under the tested stress conditions, A. ferrooxidans cells became elongated while the Fourier transform infrared spectroscopy (FT-IR) analysis showed alterations in the wavenumbers between 850 and 1,275 cm-1, which are related to carbohydrates, phospholipids and phosphoproteins. These findings indicate that the bacterial cell surface is affected by the tested stress conditions. A proteomic analysis, using 2-DE and tandem mass spectrometry, enabled the identification of 44 differentially expressed protein spots, being 30 due to heat stress (40°C) and 14 due to K2HPO4 starvation. The identified proteins belonged to 11 different functional categories, including protein fate, energy metabolism and cellular processes. The upregulated proteins were mainly from protein fate and energy metabolism categories. The obtained results provide evidences that A. ferrooxidans LR responds to heat stress and K2HPO4 starvation by inducing alterations in cellular morphology and chemical composition of the cell surface. Also, the identification of several proteins involved in protein fate suggests that the bacteria cellular homesostasis was affected. 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