Studying the Impact of Trained Staff on Evacuation Scenarios by Agent-Based Simulation

Human evacuation experiments can trigger distress, be unethical and present high costs. As a solution, computer simulations can predict the effectiveness of new emergency management procedures. This paper applies multi-agent simulation to measure the influence of staff members with diverse training levels on evacuation time. A previously developed and validated model was extended with explicit mechanisms to simulate staff members helping people to egress. The majority of parameter settings have been based on empirical data acquired in earlier studies. Therefore, simulation results are expected to be realistic. Results show that staff are more effective in complex environments, especially when trained. Not only specialised security professionals but, especially, regular workers of shopping facilities and offices play a significant role in evacuation processes when adequately trained. These results can inform policy makers and crowd managers on new emergency management procedures

Broader implications of SILAC-based proteomics for dissecting signaling dynamics in cancer

Large-scale transcriptome and epigenome analyses have been widely utilized to discover gene alterations implicated in cancer development at the genetic level. However, mapping of signaling dynamics at the protein level is likely to be more insightful and needed to complement massive genomic data. Stable isotope labeling with amino acids in cell culture (SILAC)-based proteomic analysis represents one of the most promising comparative quantitative methods that has been extensively employed in proteomic research. This technology allows for global, robust and confident identification and quantification of signal perturbations important for the progress of human diseases, particularly malignancies. The present review summarizes the latest applications of in vitro and in vivo SILAC-based proteomics in identifying global proteome/phosphoproteome and genome-wide protein-protein interactions that contribute to oncogenesis, highlighting the recent advances in dissecting signaling dynamics in cancer