Anomalia megadolicobasilar: a propósito de 5 casos diagnosticados angiograficamente

Após breves considerações sobre a sintomatologia e etiopatogenia da megadolicobasilar, são apresentados 5 casos desta anomalia vascular. Concordando com a maioria dos trabalhos sobre o assunto, a idade dos doentes desta série situava-se entre a quarta e sexta décadas da vida, apresentando todos hipertensão arterial de longa duração e aterosclerose avançada; o quadro neurológico deste doentes mostrou-se variável, tendo em comum o fato de ter se iniciado abruptamente. O estudo angiográfico do sistema vértebro-basilar foi o exame que possibilitou o diagnóstico em todos os casos

Serodiagnosis of Babesia equi in horses submitted to exercise stress Diagnóstico sorológico de infestação por Babesia equi em eqüinos submetidos ao estresse do exercício

A complement fixation test (CFT), performed in microtitre plates, based upon the use of crude antigenic preparation of Babesia equi was adapted for the detection of antibodies in serum of infected horses. The indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) were also used for the immunodiagnosis of B. equi. Serum samples from 15 apparently healthy horses, previously conditioned to a high-speed equine treadmill, were taken before and after exercise. All the samples analyzed were positive for B. equi infection. There were no significant differences (P<0.01) between these 3 tests, or the condition of rest or stress. The combined use of CFT and IFAT or ELISA should be recommended in order to enable veterinary services to more efficiently prevent introduction of infected horses into disease-free areas.<br>A reação de fixação do complemento (RFC), realizada em microplacas, utilizando-se antígeno bruto de Babesia equi, foi adaptada para a detecção de anticorpos em soros de eqüinos infectados. A reação de imunofluorescência indireta (RIFI) e o ensaio imunoenzimático (ELISA) também foram utilizados para o imunodiagnóstico de B. equi. Amostras de soro foram obtidas de 15 eqüinos aparentemente sadios, submetidos a treinamento físico em esteira rolante de alto desempenho, sendo as amostras colhidas antes e após os exercícios. Todas as amostras testadas foram positivas para B. equi. Não houve diferença significativa (P<0,01) entre estes 3 testes sorológicos, ou entre a condição de estresse e repouso. A combinação da RFC com a RIFI ou ELISA pode ser recomendada a fim de evitar a entrada de eqüinos portadores em áreas consideradas livres da doença

Quantitative trait locus analysis of wheat quality traits

Milling and baking quality traits in wheat (Triticum aestivum L.) were studied by QTL analysis in the ITMI population, a set of 114 recombinant inbred lines (RILs) generated from a synthetic-hexaploid (W7985) x bread-wheat (Opata 85) cross. Grain from RILs grown in U.S., French, and Mexican wheat-growing regions was assayed for kernel-texture traits, protein concentration and quality, and dough strength and mixing traits. Only kernel-texture traits showed similar genetic control in all environments, with Opata ha alleles at the hardness locus Ha on chromosome arm 5DS increasing grain hardness, alkaline water retention capacity, and flour yield. Dough strength was most strongly influenced by Opata alleles at 5DS loci near or identical to Ha. Grain protein concentration was associated not with high-molecular-weight glutenin loci but most consistently with the Gli-D2 gliadin locus on chromosome arm 6DS. In Mexican-grown material, a 2DS locus near photoperiod-sensitivity gene Ppd1 accounted for 25% of variation in protein, with the ppd1-coupled allele associated with higher (1.1%) protein concentration. Mixogram traits showed most influence from chromosomal regions containing gliadin or low-molecular-weight glutenin loci on chromosome arms 1AS, 1BS, and 6DS, with the synthetic hexaploid contributing favorable alleles. Some RI lines showed quality values consistently superior to those of the parental material, suggesting the potential of further evaluating new combinations of alleles from diploid and tetraploid relatives, especially alleles of known storage proteins, for improvement of quality traits in wheat cultivars