Quantitative evaluation of motor function before and after engraftment of dopaminergic neurons in a rat model of Parkinson's disease

Although gait change is considered a useful indicator of severity in animal models of Parkinson's disease, systematic and extensive gait analysis in animal models of neurological deficits is not well established. The CatWalk-assisted automated gait analysis system provides a comprehensive way to assess a number of dynamic and static gait parameters simultaneously. In this study, we used the Catwalk system to investigate changes in gait parameters in adult rats with unilateral 6-OHDA-induced lesions and the rescue effect of dopaminergic neuron transplantation on gait function. Four weeks after 6-OHDA injection, the intensity and maximal area of contact were significantly decreased in the affected paws and the swing speed significantly decreased in all four paws. The relative distance between the hind paws also increased, suggesting that animals with unilateral 6-OHDA-induced lesions required all four paws to compensate for loss of balance function. At 8 weeks post-transplantation, engrafted dopaminergic neurons expressed tyrosine hydroxylase. In addition, the intensity, contact area, and swing speed of the four limbs increased and the distance between the hind paws decreased. Partial recovery of methamphetamine-induced rotational response was also noted

Variable Structure Control of a Hand-Launched Glider

Variable structure control system design methods are applied to the problem of aircraft spin recovery. A variable structure control law typically has two phases of operation. The reaching mode phase uses a nonlinear relay control strategy to drive the system trajectory to a pre-defined switching surface within the motion state space. The sliding mode phase involves motion along the surface as the system moves toward an equilibrium or critical point. Analysis results presented in this paper reveal that the conventional method for spin recovery can be interpreted as a variable structure controller with a switching surface defined at zero yaw rate. Application of Lyapunov stability methods show that deflecting the ailerons in the direction of the spin helps to insure that this switching surface is stable. Flight test results, obtained using an instrumented hand-launched glider, are used to verify stability of the reaching mode dynamics

The flux pinning force and vortex phase diagram of single crystal FeTe0.60Se0.40

The flux pinning force density (Fp) of the single crystalline FeTe0.60Se0.40 superconductor has been calculated from the magnetization measurements. The normalized Fp versus h (=H/Hirr) curves are scaled using the Dew-Hughes formula to underline the pinning mechanism in the compound. The obtained values of pinning parameters p and q indicate the vortex pinning by the mixing of the surface and the point core pinning of the normal centers. The vortex phase diagram has also been drawn for the first time for the FeTe0.60Se0.40, which has very high values of critical current density Jc ~ 1.10(5) Amp/cm2 and the upper critical field Hc2(0) = 65T, with a reasonably high transition temperature Tc =14.5K.Comment: 12 pages, 4 figure

Intelligent tracking control of a DC motor driver using self-organizing TSK type fuzzy neural networks

[[abstract]]In this paper, a self-organizing Takagi–Sugeno–Kang (TSK) type fuzzy neural network (STFNN) is proposed. The self-organizing approach demonstrates the property of automatically generating and pruning the fuzzy rules of STFNN without the preliminary knowledge. The learning algorithms not only extract the fuzzy rule of STFNN but also adjust the parameters of STFNN. Then, an adaptive self-organizing TSK-type fuzzy network controller (ASTFNC) system which is composed of a neural controller and a robust compensator is proposed. The neural controller uses an STFNN to approximate an ideal controller, and the robust compensator is designed to eliminate the approximation error in the Lyapunov stability sense without occurring chattering phenomena. Moreover, a proportional-integral (PI) type parameter tuning mechanism is derived to speed up the convergence rates of the tracking error. Finally, the proposed ASTFNC system is applied to a DC motor driver on a field-programmable gate array chip for low-cost and high-performance industrial applications. The experimental results verify the system stabilization and favorable tracking performance, and no chattering phenomena can be achieved by the proposed ASTFNC scheme.[[notice]]補正完畢[[incitationindex]]SCI[[booktype]]紙本[[booktype]]電子

Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey

<p>Abstract</p> <p>Background</p> <p>Orchids comprise one of the largest families of flowering plants and generate commercially important flowers. However, model plants, such as <it>Arabidopsis thaliana </it>do not contain all plant genes, and agronomic and horticulturally important genera and species must be individually studied.</p> <p>Results</p> <p>Several molecular biology tools were used to isolate flower-specific gene promoters from <it>Oncidium </it>'Gower Ramsey' (<it>Onc</it>. GR). A cDNA library of reproductive tissues was used to construct a microarray in order to compare gene expression in flowers and leaves. Five genes were highly expressed in flower tissues, and the subcellular locations of the corresponding proteins were identified using lip transient transformation with fluorescent protein-fusion constructs. BAC clones of the 5 genes, together with 7 previously published flower- and reproductive growth-specific genes in <it>Onc</it>. GR, were identified for cloning of their promoter regions. Interestingly, 3 of the 5 novel flower-abundant genes were putative trypsin inhibitor (<it>TI</it>) genes (<it>OnTI1</it>, <it>OnTI2 </it>and <it>OnTI3</it>), which were tandemly duplicated in the same BAC clone. Their promoters were identified using transient GUS reporter gene transformation and stable <it>A. thaliana </it>transformation analyses.</p> <p>Conclusions</p> <p>By combining cDNA microarray, BAC library, and bombardment assay techniques, we successfully identified flower-directed orchid genes and promoters.</p

Staggered flux and stripes in doped antiferromagnets

We have numerically investigated whether or not a mean-field theory of spin textures generate fictitious flux in the doped two dimensional $t-J$-model. First we consider the properties of uniform systems and then we extend the investigation to include models of striped phases where a fictitious flux is generated in the domain wall providing a possible source for lowering the kinetic energy of the holes. We have compared the energetics of uniform systems with stripes directed along the (10)- and (11)-directions of the lattice, finding that phase-separation generically turns out to be energetically favorable. In addition to the numerical calculations, we present topological arguments relating flux and staggered flux to geometric properties of the spin texture. The calculation is based on a projection of the electron operators of the $t-J$ model into a spin texture with spinless fermions.Comment: RevTex, 19 pages including 20 figure

A family case of fertile human 45,X,psu dic(15;Y) males

We report on a familial case including four male probands from three generations with a 45,X,psu dic(15;Y)(p11.2;q12) karyotype. 45,X is usually associated with a female phenotype and only rarely with maleness, due to translocation of small Y chromosomal fragments to autosomes. These male patients are commonly infertile because of missing azoospermia factor regions from the Y long arm. In our familial case we found a pseudodicentric translocation chromosome, that contains almost the entire chromosomes 15 and Y. The translocation took place in an unknown male ancestor of our probands and has no apparent effect on fertility and phenotype of the carrier. FISH analysis demonstrated the deletion of the pseudoautosomal region 2 (PAR2) from the Y chromosome and the loss of the nucleolus organizing region (NOR) from chromosome 15. The formation of the psu dic(15;Y) chromosome is a reciprocal event to the formation of the satellited Y chromosome (Yqs). Statistically, the formation of 45,X,psu dic(15;Y) (p11.2;q12) is as likely as the formation of Yqs. Nevertheless, it has not been described yet. This can be explained by the dicentricity of this translocation chromosome that usually leads to mitotic instability and meiotic imbalances. A second event, a stable inactivation of one of the two centromeres is obligatory to enable the transmission of the translocation chromosome and thus a stably reduced chromosome number from father to every son in this family

Seasonal variation and spatial distribution of carbonaceous aerosols in Taiwan

To investigate the physico-chemical properties of aerosols in Taiwan, an observation network was initiated in 2003. In this work, the measurements of the mass concentration and carbonaceous composition of PM(10) and PM(2.5) are presented. Analysis on the data collected in the first 5-years, from 2003 to 2007, showed that there was a very strong contrast in the aerosol concentration and composition between the rural and the urban/suburban stations. The five-year means of EC at the respective stations ranged from 0.9 +/- 0.04 to 4.2 +/- 0.1 mu gC m(-3). In rural areas, EC accounted for 2-3% of PM(10) and 3-5% of PM(2.5) mass loadings, comparing to 4-6% of PM(10) and 4-8% of PM(2.5) in the urban areas. It was found that the spatial distribution of EC was consistent with CO and NO(x) across the network stations, suggesting that the levels of EC over Taiwan were dominated by local sources. The measured OC was split into POC and SOC counterparts following the EC tracer method. Five-year means of POC ranged from 1.8 +/- 0.1 to 9.7 +/- 0.2 mu gC m(-3) among the stations. It was estimated that the POM contributed 5-17% of PM(10) and 7-18% of PM(2.5) in Taiwan. On the other hand, the five-year means of SOC ranged from 1.5 +/- 0.1 to 3.8 +/- 0.3 mu gC m(-3). The mass fractions of SOM were estimated to be 9-19% in PM(10) and 14-22% in PM(2.5). The results showed that the SOC did not exhibit significant urban-rural contrast as did the POC and EC. A significant cross-station correlation between SOC and total oxidant was observed, which means the spatial distribution of SOC in Taiwan was dominated by the oxidant mixing ratio. Besides, correlation was also found between SOC and particulate nitrate, implying that the precursors of SOA were mainly from local anthropogenic sources. In addition to the spatial distribution, the carbonaceous aerosols also exhibited distinct seasonality. In northern Taiwan, the concentrations of all the three carbonaceous components (EC, POC, and SOC) reached their respective minima in the fall season. POC and EC increased drastically in winter and peaked in spring, whereas the SOC was characterized by a bimodal pattern with the maximal concentration in winter and a second mode in summertime. In southern Taiwan, minimal levels of POC and EC occurred consistently in summer and the maxima were observed in winter, whereas the SOC peaked in summer and declined in wintertime. The discrepancies in the seasonality of carbonaceous aerosols between northern and southern Taiwan were most likely caused by the seasonal meteorological settings that dominated the dispersion of air pollutants. Moreover, it was inferred that the Asian pollution outbreaks could have shifted the seasonal maxima of air pollutants from winter to spring in the northern Taiwan, and that the increases in biogenic SOA precursors and the enhancement in SOA yield were responsible for the elevated SOC concentrations in summer

DDW Order and its Role in the Phase Diagram of Extended Hubbard Models

We show in a mean-field calculation that phase diagrams remarkably similar to those recently proposed for the cuprates arise in simple microscopic models of interacting electrons near half-filling. The models are extended Hubbard models with nearest neighbor interaction and correlated hopping. The underdoped region of the phase diagram features $d_{{x^2}-{y^2}}$ density-wave (DDW) order. In a certain regime of temperature and doping, DDW order coexists with antiferromagnetic (AF) order. For larger doping, it coexists with $d_{{x^2}-{y^2}}$ superconductivity (DSC). While phase diagrams of this form are robust, they are not inevitable. For other reasonable values of the coupling constants, drastically different phase diagrams are obtained. We comment on implications for the cuprates.Comment: 7 pages, 3 figure

Flux Compactifications on Calabi-Yau Threefolds

The presence of RR and NS three-form fluxes in type IIB string compactification on a Calabi-Yau orientifold gives rise to a nontrivial superpotential W for the dilaton and complex structure moduli. This superpotential is computable in terms of the period integrals of the Calabi-Yau manifold. In this paper, we present explicit examples of both supersymmetric and nonsupersymmetric solutions to the resulting 4d N=1 supersymmetric no-scale supergravity, including some nonsupersymmetric solutions with relatively small values of W. Our examples arise on orientifolds of the hypersurfaces in $WP^{4}_{1,1,1,1,4}$ and $WP^{4}_{1,1,2,2,6}$. They serve as explicit illustrations of several of the ingredients which have played a role in the recent proposals for constructing de Sitter vacua of string theory.Comment: 30 pages, harvmac big; refs and minor comments adde