82 research outputs found
Shelf life determination of durian (Durio zibethinus) paste and pulp upon highpressure processing
Effect of high pressure processing on the microbiological, physicochemical and enzymatic properties of jackfruit (Artocarpus heterophyllus L.) bulb
The holographic superconductors in higher-dimensional AdS soliton
We explore the behaviors of the holographic superconductors at zero
temperature for a charged scalar field coupled to a Maxwell field in
higher-dimensional AdS soliton spacetime via analytical way. In the probe
limit, we obtain the critical chemical potentials increase linearly as a total
dimension grows up. We find that the critical exponent for condensation
operator is obtained as 1/2 independently of , and the charge density is
linearly related to the chemical potential near the critical point.
Furthermore, we consider a slightly generalized setup the
Einstein-Power-Maxwell field theory, and find that the critical exponent for
condensation operator is given as in terms of a power parameter
of the Power-Maxwell field, and the charge density is proportional to the
chemical potential to the power of .Comment: LaTeX, 16 pages, 5 figures, typos corrected, one reference added,
version to appear in European Physical Journal
Geometries with Killing Spinors and Supersymmetric AdS Solutions
The seven and nine dimensional geometries associated with certain classes of
supersymmetric and solutions of type IIB and D=11 supergravity,
respectively, have many similarities with Sasaki-Einstein geometry. We further
elucidate their properties and also generalise them to higher odd dimensions by
introducing a new class of complex geometries in dimensions, specified
by a Riemannian metric, a scalar field and a closed three-form, which admit a
particular kind of Killing spinor. In particular, for , we show that
when the geometry in dimensions is a cone we obtain a class of
geometries in dimensions, specified by a Riemannian metric, a scalar
field and a closed two-form, which includes the seven and nine-dimensional
geometries mentioned above when , respectively. We also consider various
ansatz for the geometries and construct infinite classes of explicit examples
for all .Comment: 28 page
Charmless hadronic decays and new physics effects in the general two-Higgs doublet models
Based on the low-energy effective Hamiltonian with the generalized
factorization, we calculate the new physics contributions to the branching
ratios of the two-body charmless hadronic decays of and mesons
induced by the new gluonic and electroweak charged-Higgs penguin diagrams in
the general two-Higgs doublet models (models I, II and III). Within the
considered parameter space, we find that: (a) the new physics effects from new
gluonic penguin diagrams strongly dominate over those from the new -
and - penguin diagrams; (b) in models I and II, new physics contributions
to most studied B meson decay channels are rather small in size: from -15% to
20%; (c) in model III, however, the new physics enhancements to the
penguin-dominated decay modes can be significant, , and
therefore are measurable in forthcoming high precision B experiments; (d) the
new physics enhancements to ratios {\cal B}(B \to K \etap) are significant in
model III, , and hence provide a simple and plausible new
physics interpretation for the observed unexpectedly large B \to K \etap
decay rates; (e) the theoretical predictions for and
in model III are still consistent with the data
within errors; (f) the significant new physics enhancements to the
branching ratios of and decays are helpful to improve the
agreement between the data and the theoretical predictions; (g) the theoretical
predictions of in the 2HDM's are generally
consistent with experimental measurements and upper limits ()Comment: 55 pages, Latex file, 17 PS and EPS figures. With minor corrections,
final version to be published in Phys.Rev. D. Repot-no: PKU-TH-2000-4
Characterization of anticoagulant heparinoids by immunoprofiling
Heparinoids are used in the clinic as anticoagulants. A specific pentasaccharide in heparinoids activates antithrombin III, resulting in inactivation of factor Xa and–when additional saccharides are present–inactivation of factor IIa. Structural and functional analysis of the heterogeneous heparinoids generally requires advanced equipment, is time consuming, and needs (extensive) sample preparation. In this study, a novel and fast method for the characterization of heparinoids is introduced based on reactivity with nine unique anti-heparin antibodies. Eight heparinoids were biochemically analyzed by electrophoresis and their reactivity with domain-specific anti-heparin antibodies was established by ELISA. Each heparinoid displayed a distinct immunoprofile matching its structural characteristics. The immunoprofile could also be linked to biological characteristics, such as the anti-Xa/anti-IIa ratio, which was reflected by reactivity of the heparinoids with antibodies HS4C3 (indicative for 3-O-sulfates) and HS4E4 (indicative for domains allowing anti-factor IIa activity). In addition, the immunoprofile could be indicative for heparinoid-induced side-effects, such as heparin-induced thrombocytopenia, as illustrated by reactivity with antibody NS4F5, which defines a very high sulfated domain. In conclusion, immunoprofiling provides a novel, fast, and simple methodology for the characterization of heparinoids, and allows high-throughput screening of (new) heparinoids for defined structural and biological characteristics
Integrated multi-omics for rapid rare disease diagnosis on a national scale
Published online: 8 June 2023Critically ill infants and children with rare diseases need equitable access to rapid and accurate diagnosis to direct clinical management. Over 2 years, the Acute Care Genomics program provided whole-genome sequencing to 290 families whose critically ill infants and children were admitted to hospitals throughout Australia with suspected genetic conditions. The average time to result was 2.9 d and diagnostic yield was 47%. We performed additional bioinformatic analyses and transcriptome sequencing in all patients who remained undiagnosed. Long-read sequencing and functional assays, ranging from clinically accredited enzyme analysis to bespoke quantitative proteomics, were deployed in selected cases. This resulted in an additional 19 diagnoses and an overall diagnostic yield of 54%. Diagnostic variants ranged from structural chromosomal abnormalities through to an intronic retrotransposon, disrupting splicing. Critical care management changed in 120 diagnosed patients (77%). This included major impacts, such as informing precision treatments, surgical and transplant decisions and palliation, in 94 patients (60%). Our results provide preliminary evidence of the clinical utility of integrating multi-omic approaches into mainstream diagnostic practice to fully realize the potential of rare disease genomic testing in a timely manner.Sebastian Lunke ... Peer Arts ... Christopher P. Barnett ..., Chirag V. Patel ... Hamish S. Scott ... Karin S. Kassahn ... et al
Splenic lymphoma with villus lymphocytes - An uncommon cause for lymphocytosis
Singapore Medical Journal389395-398SIMJ
A novel nuclease-ATPase (Nar71) from archaea is part of a proposed thermophilic DNA repair system
We have identified a novel structure-specific nuclease in highly fractionated extracts of the thermophilic archaeon Methanothermobacter thermautotrophicus (Mth). The 71 kDa protein product of open reading frame mth1090 is a nuclease with ATPase activity, which we call Nar71 (Nuclease-ATPase in Repair, 71 kDa). The nar71 gene is located in a gene neighbourhood proposed by genomics to encode a novel DNA repair system conserved in thermophiles. The biochemical characterization of Nar71 presented here is the first analysis from within this neighbourhood, and it supports the insight from genomics. Nuclease activity of Nar71 is specific for 3' flaps and flayed duplexes, targeting single-stranded DNA (ssDNA) regions. This activity requires Mg2+ or Mn2+ and is greatly reduced in ATP. In ATP, Nar71 displaces ssDNA, also with high specificity for 3' flap and flayed duplex DNA. Strand displacement is weak compared with nuclease activity, but in ATPS it is abolished, suggesting that Nar71 couples ATP hydrolysis to DNA strand separation. ATPase assays confirmed that Nar71 is stimulated by ssDNA, though not double-stranded DNA. Mutation of Lys-117 in Nar71 abolished ATPase and nuclease activity, and we describe a separation-of-function mutant (K68A) that has lost ATPase activity but retains nuclease activity. A model of possible Nar71 function in DNA repair is presented
Analysis of priority substituted phenols by micellar electrokinetic chromatography
Environmental Monitoring and Assessment191-393-103EMAS
- …