Little difference between minimum inhibitory concentrations of Mycobacterium tuberculosis wild-type organisms determined with BACTEC MGIT 960 and Middlebrook 7H10

AbstractThe MIC wild-type (WT) distribution for Mycobacterium tuberculosis in BACTEC 960 MGIT is not defined, which may result in poor reproducibility for drug susceptibility testing (DST), as several DST methods with different breakpoints are in use. In a comparison between MGIT and Middlebrook 7H10 medium of seven first- and second-line drugs, including 133 MIC determinations of 15 WT isolates, we found an agreement of 91.7% within ± one MIC dilution step. The results confirm the agreement in MIC testing between 7H10 and MGIT and indicate that breakpoints could be harmonized in order to avoid misclassification

Dimensional Reduction of Fermions in Brane Worlds of the Gross-Neveu Model

We study the dimensional reduction of fermions, both in the symmetric and in the broken phase of the 3-d Gross-Neveu model at large N. In particular, in the broken phase we construct an exact solution for a stable brane world consisting of a domain wall and an anti-wall. A left-handed 2-d fermion localized on the domain wall and a right-handed fermion localized on the anti-wall communicate with each other through the 3-d bulk. In this way they are bound together to form a Dirac fermion of mass m. As a consequence of asymptotic freedom of the 2-d Gross-Neveu model, the 2-d correlation length \xi = 1/m increases exponentially with the brane separation. Hence, from the low-energy point of view of a 2-d observer, the separation of the branes appears very small and the world becomes indistinguishable from a 2-d space-time. Our toy model provides a mechanism for brane stabilization: branes made of fermions may be stable due to their baryon asymmetry. Ironically, our brane world is stable only if it has an extreme baryon asymmetry with all states in this ``world'' being completely filled.Comment: 26 pages, 7 figure

The threshold at which substrate nanogroove dimensions may influence fibroblast alignment and adhesion.

Contains fulltext : 36616.pdf (publisher's version ) (Closed access)The differences in morphological behaviour between fibroblasts cultured on smooth and nanogrooved substrata (groove depth: 5-350 nm, width: 20-1000 nm) have been evaluated in vitro. The aim of the study was to clarify to what extent cell guidance occurs on increasingly smaller topographies. Pattern templates were made using electron beam lithography, and were subsequently replicated in polystyrene cell culture material using solvent casting. The replicates were investigated with atomic force microscopy (AFM). After seeding with fibroblasts, morphological characteristics were investigated using scanning electron microscopy (SEM) and light microscopy, in order to obtain qualitative and quantitative information on cell alignment. AFM revealed that the nanogroove/ridge widths were replicated perfectly, although at deeper levels the grooves became more concave. The smooth substrata had no distinguishable pattern other than a roughness amplitude of 1 nm. Interestingly, microscopy and image analysis showed that fibroblast after 4 h had adjusted their shape according to nanotopographical features down to cut-off values of 100 nm width and 75 nm depth. After 24 h culturing time, fibroblasts would even align themselves on groove depths as shallow as 35 nm. It appears depth is the most essential parameter in cellular alignment on groove patterns with a pitch ratio of 1:1. On the smooth substrata, cells always spread out in a random fashion. Analysis of variance (ANOVA) demonstrated that both main parameters, topography and culturing time, were significant. We conclude that fibroblast cells cultured on nanotopography experience a threshold feature size of 35 nm, below this value contact guidance does no longer exist.8 p