The effect of salts on the derivatization and chromatography of amino acids

Effect of salts on derivatization and chromatography of amino acid

The hydrolysis of proteins

Temperature effects on hydrolysis reaction in protein amino acid

Gas-liquid chromatography of amino acids in biological substances

Developing gas-liquid chromatographic method for quantitative analysis of amino acids in blood plasma and urin

The search for an identification of amino acids, nucleobases and nucleosides in samples returned from Mars

The Mars Sample Return mission will provide us with a unique source of material from our solar system; material which could advance our knowledge of the processes of chemical evolution. As has been pointed out, Mars geological investigations based on the Viking datasets have shown that primordial Mars was in many biologically important ways similar to the primordial Earth; the presence of surface liquid water, moderate surface temperatures, and atmosphere of carbon dioxide and nitrogen, and high geothermal heat flow. Indeed, it would seem that conditions on Earth and Mars were fundamentally similar during the first one billion years or so. As has been pointed out, Mars may well contain the best preserved record of the events that transpired on the early planets. Examination of that early record will involve searching for many things, from microfossils to isotopic abundance data. We propose an investigation of the returned Mars samples for biologically important organic compounds, with emphases on amino acids, the purine and pyrimidine bases, and nucleosides

The search for and identification of amino acids, nucleobases and nucleosides in samples returned from Mars

An investigation of the returned Mars samples for biologically important organic compounds, with emphasis on amino acid, the puring and pyrimidine bases, and nucleosides is proposed. These studies would be conducted on subsurface samples obtained by drilling past the surface oxidizing layer with emphasis on samples containing the larges quantities of organic carbon as determined by the rover gas chromatographic mass spectrometer (GCMS). Extraction of these molecules from the returned samples will be performed using the hydrothermal extraction technique described by Cheng and Ponnamperuma. More rigorous extraction methods will be developed and evaluated. For analysis of the extract for free amino acids or amino acids present in a bound or peptidic form, aliquots will be analyzed by capillary GCMS both before and after hydrolysis with 6N hydrochloric acid. Establishment of the presence of amino acids would then lead to the next logical step which would be the use of chiral stationary gas chromatography phases to determine the enatiomeic composition of the amino acids present, and thus potentially establish their biotic or abiotic origin. Confirmational analyses for amino acids would include ion-exchange and reversed-phase liquid chromatographic analysis. For analyses of the returned Mars samples for nucleobases and nucleosides, affinity and reversed-phase liquid chromatography would be utilized. This technology coupled with scanning UV detection for identification, presents a powerful tool for nucleobase and nucleoside analysis. Mass spectrometric analysis of these compounds would confirm their presence in samples returned form Mars

A manned exobiology laboratory based on the Moon

Establishment of an exobiology laboratory on the Moon would provide a unique opportunity for exploration of extraterrestrial materials on a long-term, ongoing basis, for elucidation of exobiological processes and chemical evolution. A major function of the lunar exobiology laboratory would be to examine samples collected from other planets (e.g., Mars) for the presence of extant or extinct life. By establishing a laboratory on the Moon, preliminary analyses could be conducted away from Earth, thus establishing that extraterrestrial materials are benign before their return to Earth for more extensive investigations. The Moon-based exobiology laboratory would have three major components for study of samples returned from other planets: (1) the search for extant life - this component would focus on the detection and identification of life forms using biological, physical, and chemical methods; (2) the search for extinct life - this component would concentrate on identification of extinct life using micropaleontological physical and chemical means; and (3) the search and evidence of chemical evolution - this component would be devoted to the detection and identification of molecules revealing prebiotic chemical evolution

A direct disodium dihydrogen ethylenediaminetetraacetate titration procedure for magnesium in limestone

Digitized 2007 AES.Includes bibliographical references (page 10)

Direct ethylenediaminetetraacetate titration procedure for calcium in biological substances : anion exchange separation of phosphate

Digitized 2007 AES.Includes bibliographical references (page 10)

A modified BlÄzka–type respirometer for the study of swimming metabolism in fishes having deep, laterally compressed bodies or unusual locomotor modes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73888/1/j.1095-8649.2000.tb00890.x.pd