MOLECULAR, GENETIC AND BIOCHEMICAL CHARACTERIZATION OF OLEIC ACID- AND GLYCEROL-MEDIATED SIGNALING IN PLANT DEFENSE

Oleic acid (18:1) is one of the important monounsaturated fatty acids, which is synthesized upon desaturation of stearic acid and this reaction is catalyzed by the SSI2 encoded stearoyl-acyl-carrier-protein-desaturase. A mutation in SSI2 leads to constitutive activation of salicylic acid (SA)-mediated defense responses. Consequently, these plants accumulate high levels of SA and show enhanced resistance to bacterial and oomycete pathogens. Replenishing 18:1 levels in ssi2 plants, via a second site mutation in GLY1 encoded glycerol-3-phosphate (G3P) dehydrogenase, suppresses all the ssi2-triggered phenotypes. Study of mechanism(s) underlying gly1-mediated suppression of ssi2 phenotypes showed that 18:1 levels are regulated via acylation with G3P and a balance between G3P and 18:1 is critical for the regulation of defense signaling pathways. To establish a role for 18:1 and G3P during host defense, interaction between Colletotrichum higginsianum and Arabidopsis was studied. Resistance to C. higginsianum correlated with host G3P levels. The gly1 plants showed increased susceptibility while act1 plants, defective in utilization of G3P, showed enhanced resistance. Plant overexpessing GLY1 showed enhanced resistance in both wild type as well as camalexin deficient backgrounds. Together, these results suggested that G3P conferred resistance acted downstream or independent of camalexin. Exogenous application of glycerol lowered 18:1 levels and produced ssi2-like phenotypes in wild-type plants. Furthermore, glycerol application or the ssi2 mutation produced similar phenotypes in fatty acid desaturation mutants and mutants defective in SA/resistance gene signaling. Expression studies showed that ssi2 phenotypes were likely due to increased expression of resistance genes. Epistatic analysis suggested that certain components of SA pathway had redundant function and were required for 18:1-regulated signaling

Drag and inertia coefficients for horizontally submerged rectangular cylinders in waves and currents

The results of an experimental investigation carried out to measure combined wave and current loads on horizontally submerged square and rectangular cylinders are reported in this paper. The wave and current induced forces on a section of the cylinders with breadth-depth (aspect) ratios equal to 1, 0.5, and 0.75 are measured in a wave tank. The maximum value of Keulegan-Carpenter (KC) number obtained in waves alone is about 5 and Reynolds (Re) number ranged from 6.3976103 to 1.186105. The drag (CD) and inertia (CM) coefficients for each cylinder are evaluated using measured sectional wave forces and particle kinematics calculated from linear wave theory. The values of CD and CM obtained for waves alone have already been reported (Venugopal, V., Varyani, K. S., and Barltrop, N. D. P. Wave force coefficients for horizontally submerged rectangular cylinders. Ocean Engineering, 2006, 33, 11-12, 1669-1704) and the coefficients derived in combined waves and currents are presented here. The results indicate that both drag and inertia coefficients are strongly affected by the presenceof the current and show different trends for different cylinders. The values of the vertical component inertia coefficients (CMY) in waves and currents are generally smaller than the inertia coefficients obtained in waves alone, irrespective of the current's magnitude and direction. The results also illustrate the effect of a cylinder's aspect ratio on force coefficients. This study will be useful in the design of offshore structures whose columns and caissons are rectangular sections

A Web-based GIS for Tourism Administration in Kerala

Geographic  Information  systems  are  used  widely  for Tourism  administration  in  several  countries,  but  not especially true in Kerala scenario. This paper attempts to present a GIS model for tourism administration in Kerala. Various  applications  of  Tourism  based  GIS  in  the international  arena  have  been  examined  in  this  paper. Data collected from different sources reveals the present status of Tourism GISs world over. The study focuses on areas where GIS can be applied in Kerala Tourism and gives outputs that can be generated using the proposed model.  Tourism  is  essentially  a  cartographic phenomenon.  In  this  context  this  paper  assumes importance as various devices, both wired and wireless are  increasingly  used  to  accesses  cartographic information.  With  internet  becoming  an  unavoidable component of modern day life, map based information is the need of the hour. A web based GIS will benefit Kerala tourism in the coming years. The model proposed in this paper can act as a foundation for building a web based GIS for Kerala tourism

Trions in a periodic potential

The group-theoretical classification of trion states is presented. It is based on considerations of products of irreducible representations of the 2D translation group. For a given BvK period N degeneracy of obtained states is N^2. Trions consist of two identical particles so the symmetrization of states with respect to particles transposition is considered. Completely antisymmetric states can be constructed by introducing antisymmetric spin functions. Two symmetry adapted bases are considered. The third possibility is postponed for the further investigations.Comment: revtex, 5 p., sub. to Physica

DRSP : Dimension Reduction For Similarity Matching And Pruning Of Time Series Data Streams

Similarity matching and join of time series data streams has gained a lot of relevance in today's world that has large streaming data. This process finds wide scale application in the areas of location tracking, sensor networks, object positioning and monitoring to name a few. However, as the size of the data stream increases, the cost involved to retain all the data in order to aid the process of similarity matching also increases. We develop a novel framework to addresses the following objectives. Firstly, Dimension reduction is performed in the preprocessing stage, where large stream data is segmented and reduced into a compact representation such that it retains all the crucial information by a technique called Multi-level Segment Means (MSM). This reduces the space complexity associated with the storage of large time-series data streams. Secondly, it incorporates effective Similarity Matching technique to analyze if the new data objects are symmetric to the existing data stream. And finally, the Pruning Technique that filters out the pseudo data object pairs and join only the relevant pairs. The computational cost for MSM is O(l*ni) and the cost for pruning is O(DRF*wsize*d), where DRF is the Dimension Reduction Factor. We have performed exhaustive experimental trials to show that the proposed framework is both efficient and competent in comparison with earlier works.Comment: 20 pages,8 figures, 6 Table

A comparative study of night-time enhancement of TEC at a low latitude station on storm and quiet nights including the local time, seasonal and solar activity dependence

International audienceThe main characteristics of night-time enhancements in TEC during magnetic storms are compared with those during quiet nights for different seasons and solar activity conditions at Palehua, a low latitude station during the period 1980?1989. We find that the mean amplitude has both a seasonal and solar activity dependence: in winter, the values are higher for weak storms as compared to those during quiet nights and increase with an increase in solar activity. In summer, the mean amplitude values during weak storms and quiet nights are almost equal. But during equinox, the mean amplitude values for quiet nights are greater than those during weak storms. The mean half-amplitude duration is higher during weak storms as compared to that during quiet nights in summer. However, during winter and equinox, the durations are almost equal for both quiet and weak storm nights. For the mean half-amplitude duration, the quiet night values for all the seasons and equinoctial weak storm values increase with an increase in solar activity. The occurrence frequency (in percent) of TEC enhancement during weak storms is greater than during quiet nights for all seasons. The mean amplitude, the mean half-amplitude duration and the occurrence frequency (in percent) of TEC enhancement values are higher during major storms as compared to those during quiet nights. The above parameters have their highest values during pre-midnight hours. From the data analysed, this behaviour is true in the case of major storms also