Identification and Characterization of Fungal Isolates from Land-applied Sewage Sludge

Approximately eight million dry tons of sewage sludge is generated in the U.S. each year, with more than half of that now land-applied as the primary method of disposal. Despite the proliferation of this practice, little is known about the microbial constituents of these noxious materials. To address this knowledge gap, we isolated and characterized fungi present in archived samples of land-disposed sewage sludge collected from the Snoqualmie National Forest (Washington State). Sludge samples were resuspended in sterile water and 15 fungal isolates were selected and purified on sabouraud dextrose agar plates supplemented with 50 mg/L of chloramphenicol. Fungal morphology was assessed and photodocumented following growth on sabouraud dextrose agar, potato dextrose agar, yeast maltose agar, and malt extract agar. Additionally, pH and temperature tolerance was assessed by growth in liquid cultures of sabouraud dextrose broth from 0 ºC to 50 ºC and determined from pH 2 to 11 at 28 ºC. Carbon source utilization was assessed using Biolog Filamentous Fungi plates. Amplification and sequencing of the ITS region, commonly used in fungal phylogenetic analysis, is in progress to identify each isolate. Phenotypic assessments of growth, carbon utilization, and lactophenol cotton blue staining revealed that sludge-associated fungi are quite varied in size and appearance, but commonly grow in a mesophilic range (10-40 ºC and pH 4-10). Interspecies variation is further evident in the percentage of 31 carbon sources utilized (63-100%). To our knowledge, this work represents the first reported assessment of the fungal community in sewage sludge wastes disposed in the Snoqualmie National Forest

Characterizing the Volatilome of Land-disposed Sewage Sludge Under Seasonal Temperature Regimes

About eight million dry tons of sewage sludge waste is generated in the US annually, with more than half of that now land-disposed on agricultural and forested lands. Though containing essential plant nutrients, sludge also harbors complex mixtures of volatile organic compounds (VOCs) that result in toxic emissions therefrom. While ecotoxic impacts to sludged ecosystems are a primary concern, the stifling emissions are most obvious to and disconcerting for the public, which has led to increasing concerns for the safety of this practice. The large-scale disposal of sludge in the temperate rainforests of the Puget Sound Watershed has resulted in acute toxicity to macrobiota, and despite escalating concerns for detrimental impacts, little is known about the total VOC inventories, or “volatilomes,” of these complex wastes. To address this knowledge gap, we characterized VOC emissions from forest-disposed sewage sludge over a range of seasonal temperature regimes. We also incubated sludge samples at the more extreme 100 o C to assess the “complete volatilome.” After 1-hr incubations in gastight vials, VOCs accumulated in the headspace were sampled with a gastight syringe and analyzed with gas chromatography-mass spectrometry to generate distinct chemical fingerprints of sludge sample volatilomes over the range of temperatures. Total integrated chromatographic peak areas increased with temperature, indicating increased VOC production. Sludge volatilomes were dominated by a multitude of aliphatics and aromatics, with comparatively lesser emissions of alcohols, esters, aldehydes, terpenes, and nitrogen-, sulfur-, and halogen-containing compounds

Ethoxylated rapeseed oil derivatives as novel adjuvants for herbicides

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Self-emulsifying ethoxylates of rapeseed oil and methylated rapeseed oil as novel adjuvants for herbicides

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Ethoxylated rapeseed oil derivatives as novel adjuvants for herbicides

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CAST3M modelling of a spent fuel assembly bending during a handling accident Rod failure risk evaluation from the experimental results of spent fuel rod bending test

International audienceThe fuel handling operating rules exclude any accidental risk. However in the framework of the PRECCI R&D project, the bending of a spent fuel assembly resulting from its locking during a translation displacement is taken into account. This enabled us to develop an approach based on experiments and calculations that allows us to simulate the behaviour of an assembly under such loading. This study was carried out in CEA laboratories with the funding and the technical support of EDF. A three points bending test on a spent fuel rod segment was performed at the Laboratory for Mechanical Behaviour of Irradiated Materials (LCMI). From the experimental strength-displacement curve, a maximum failure strain, a maximum failure curvature and an equivalent constitutive equation were determined. CAST3M modelling of the fuel rod taking into account the elasto-plastic behaviour of the clad and the cracking of the UO2 fuel pellets was verified by the experimental results. Consequently, the identification of the respective contributions of the clad and of the pellets to the rod global behaviour was made possible. A two dimensional assembly with beam elements was modelled with CAST3M. The properties of the beams modelling the different parts of the assembly (top and bottom nozzle, grids) were chosen and adjusted according to their materials (zirconium alloys, steel) in order to obtain stiffness, tensile and shear behaviour, sliding and holding functions close to the experimental ones. Assembly bending calculations were performed. In order to obtain a rod integrity estimator, their maximum calculated strains and curvatures as a function of the bending angles can be compared to the failure experimental ones

CAST3M modelling of a spent fuel assembly bending during a handling accident Rod failure risk evaluation from the experimental results of spent fuel rod bending test

International audienceThe fuel handling operating rules exclude any accidental risk. However in the framework of the PRECCI R&D project, the bending of a spent fuel assembly resulting from its locking during a translation displacement is taken into account. This enabled us to develop an approach based on experiments and calculations that allows us to simulate the behaviour of an assembly under such loading. This study was carried out in CEA laboratories with the funding and the technical support of EDF. A three points bending test on a spent fuel rod segment was performed at the Laboratory for Mechanical Behaviour of Irradiated Materials (LCMI). From the experimental strength-displacement curve, a maximum failure strain, a maximum failure curvature and an equivalent constitutive equation were determined. CAST3M modelling of the fuel rod taking into account the elasto-plastic behaviour of the clad and the cracking of the UO2 fuel pellets was verified by the experimental results. Consequently, the identification of the respective contributions of the clad and of the pellets to the rod global behaviour was made possible. A two dimensional assembly with beam elements was modelled with CAST3M. The properties of the beams modelling the different parts of the assembly (top and bottom nozzle, grids) were chosen and adjusted according to their materials (zirconium alloys, steel) in order to obtain stiffness, tensile and shear behaviour, sliding and holding functions close to the experimental ones. Assembly bending calculations were performed. In order to obtain a rod integrity estimator, their maximum calculated strains and curvatures as a function of the bending angles can be compared to the failure experimental ones

Effet de préchargement à chaud sur la ténacité de l’acier de cuve de réacteur à eau pressurisée 16MND5 fortement irradié

Dans une perspective d’exploitation à long terme, l’effet de l’histoire de chargement sur la résistance à la propagation de fissures de l’acier de cuve peut être amené à être pris en compte pour des transitoires thermomécaniques de type perte de réfrigérant primaire (APRP). Dans un programme commun CEA, EDF et AREVA, un acier de cuve de réacteur a été irradié à un niveau correspondant à plus de 60 ans d’exploitation. Des essais mécaniques ont permis d’obtenir la description complète des propriétés mécaniques et de leurs évolutions avec l’irradiation. Des essais spécifiques ont confirmé l’effet d’histoire de chargement sur la ténacité de l’acier de cuve irradié, pour ce niveau de fluence et des trajets de chargements de type APRP

Warm PreStress effect on highly irradiated reactor pressure vessel steel

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