Allosteric Activation of the Par-6 PDZ Via a Partial Unfolding Transition

Proteins exist in a delicate balance between the native and unfolded states, where thermodynamic stability may be sacrificed to attain the flexibility required for efficient catalysis, binding, or allosteric control. Partition-defective 6 (Par-6) regulates the Par polarity complex by transmitting a GTPase signal through the Cdc42/Rac interaction binding PSD-95/Dlg/ZO-1 (CRIB-PDZ) module that alters PDZ ligand binding. Allosteric activation of the PDZ is achieved by local rearrangement of the L164 and K165 side chains to stabilize the interdomain CRIB:PDZ interface and reposition a conserved element of the ligand binding pocket. However, microsecond to millisecond dynamics measurements revealed that L164/K165 exchange requires a larger rearrangement than expected. The margin of thermodynamic stability for the PDZ domain is modest (∼3 kcal/mol) and further reduced by transient interactions with the disordered CRIB domain. Measurements of local structural stability revealed that tertiary contacts within the PDZ are disrupted by a partial unfolding transition that enables interconversion of the L/K switch. The unexpected participation of partial PDZ unfolding in the allosteric mechanism of Par-6 suggests that native-state unfolding may be essential for the function of other marginally stable proteins

Temperature can enhance coherent oscillations at a Landau-Zener transition

We consider sweeping a system through a Landau-Zener avoided-crossing, when that system is also coupled to an environment or noise. Unsurprisingly, we find that decoherence suppresses the coherent oscillations of quantum superpositions of system states, as superpositions decohere into mixed states. However, we also find an effect we call "Lamb-assisted coherent oscillations", in which a Lamb shift exponentially enhances the coherent oscillation amplitude. This dominates for high-frequency environments such as super-Ohmic environments, where the coherent oscillations can grow exponentially as either the environment coupling or temperature are increased. The effect could be used as an experimental probe for high-frequency environments in such systems as molecular magnets, solid-state qubits, spin-polarized gases (neutrons or He3) or Bose-condensates.Comment: 4 Pages & 4 Figs - New version: introduction extended & citations adde

Comparative study of design: application to Engineering Design

A recent exploratory study examines design processes across domains and compares them. This is achieved through a series of interdisciplinary, participative workshops. A systematic framework is used to collect data from expert witnesses who are practising designers across domains from engineering through architecture to product design and fashion, including film production, pharmaceutical drugs, food, packaging, graphics and multimedia and software. Similarities and differences across domains are described which indicate the types of comparative analysis we have been able to do from our data. The paper goes further and speculates on possible lessons for selected areas of engineering design which can be drawn from comparison with processes in other domains. As such this comparative design study offers the potential for improving engineering design processes. More generally it is a first step in creating a discipline of comparative design which aims to provide a new rich picture of design processes

Word frequency influences on the list length effect and associative memory in young and older adults

Many studies show that age deficits in memory are smaller for information supported by pre-experimental experience. Many studies also find dissociations in memory tasks between words that occur with high and low frequencies in language, but the literature is mixed regarding the extent of word frequency effects in normal ageing. We examined whether age deficits in episodic memory could be influenced by manipulations of word frequency. In Experiment 1, young and older adults studied short and long lists of high- and low-frequency words for free recall. The list length effect (the drop in proportion recalled for longer lists) was larger in young compared to older adults and for high- compared to low-frequency words. In Experiment 2, young and older adults completed item and associative recognition memory tests with high- and low-frequency words. Age deficits were greater for associative memory than for item memory, demonstrating an age-related associative deficit. High-frequency words led to better associative memory performance whilst low-frequency words resulted in better item memory performance. In neither experiment was there any evidence for age deficits to be smaller for high- relative to low-frequency words, suggesting that word frequency effects on memory operate independently from effects due to cognitive ageing

Employable knowledge: benchmarking education about standardization in the UK

For academics and students in the United Kingdom the main source of standards is via British Standards Institution (BSI). The research demonstrates that British, European and International Standards play a key role in many areas of education. In some disciplines their inclusion in the course is mandatory, e.g. in building construction and performing risk assessments of equipment. Where not a requirement, other courses successfully encourage students to understand and apply specific standards and principles from them, to their design and project work, in topic areas such as quality management and user-computer interface design. Assessment practice is a key part of learning and academics have indicated how this fitted into the learning activity, e.g. by expecting students to develop an understanding of standards and reference them in all assessed work. Likewise, students taking part in the survey also suggest that they were actively engaged with one or more standards and that their understanding was measured through an assessed activity. However, students also emphasised the importance of additional support, e.g. introduction to standards in the workplace or as part of work placements, by library staff as an information resource, and by presentations from BSI experts. Employers have also indicated the importance of students’ knowledge, understanding and appreciation of relevant standards in the right context, emphasising their desire for standards to be included more widely in the curriculum. The draft recommendations from this study were subject to International review and comment, the results from this review served to strengthen the recommendations of this work. This research was commissioned by British Standards Institution (BSI). This research was produced in association with the Department for Business, Innovation and Skills as part of its ongoing programme of support for standardization. Grateful thanks are due to all those who filled in the questionnaire or who attended the workshops

Radio Astronomical Polarimetry and the Lorentz Group

In radio astronomy the polarimetric properties of radiation are often modified during propagation and reception. Effects such as Faraday rotation, receiver cross-talk, and differential amplification act to change the state of polarized radiation. A general description of such transformations is useful for the investigation of these effects and for the interpretation and calibration of polarimetric observations. Such a description is provided by the Lorentz group, which is intimately related to the transformation properties of polarized radiation. In this paper the transformations that commonly arise in radio astronomy are analyzed in the context of this group. This analysis is then used to construct a model for the propagation and reception of radio waves. The implications of this model for radio astronomical polarimetry are discussed.Comment: 10 pages, accepted for publication in Astrophysical Journa

Electrical Control of Linear Dichroism in Black Phosphorus from the Visible to Mid-Infrared

The incorporation of electrically tunable materials into photonic structures such as waveguides and metasurfaces enables dynamic control of light propagation by an applied potential. While many materials have been shown to exhibit electrically tunable permittivity and dispersion, including transparent conducting oxides (TCOs) and III-V semiconductors and quantum wells, these materials are all optically isotropic in the propagation plane. In this work, we report the first known example of electrically tunable linear dichroism, observed here in few-layer black phosphorus (BP), which is a promising candidate for multi-functional, broadband, tunable photonic elements. We measure active modulation of the linear dichroism from the mid-infrared to visible frequency range, which is driven by anisotropic quantum-confined Stark and Burstein-Moss effects, and field-induced forbidden-to-allowed optical transitions. Moreover, we observe high BP absorption modulation strengths, approaching unity for certain thicknesses and photon energies

Word frequency influences on the list length effect and associative memory in young and older adults

Many studies show that age deficits in memory are smaller for information supported by preexperimental experience. Many studies also find dissociations in memory tasks between words that occur with high and low frequencies in language, but the literature is mixed regarding the extent of word frequency effects in normal ageing. We examined whether age deficits in episodic memory could be influenced by manipulations of word frequency. In Experiment 1, young and older adults studied short and long lists of high- and low-frequency words for free recall. The list length effect (the drop in proportion recalled for longer lists) was larger in young compared to older adults and for high- compared to low-frequency words. In Experiment 2, young and older adults completed item and associative recognition memory tests with high- and low-frequency words. Age deficits were greater for associative memory than for item memory, demonstrating an age-related associative deficit. High-frequency words led to better associative memory performance whilst low-frequency words resulted in better item memory performance. In neither experiment was there any evidence for age deficits to be smaller for high- relative to low-frequency words, suggesting that word frequency effects on memory operate independently from effects due to cognitive ageing