17 research outputs found
Recent developments in application of solid phase clean-up procedures for rapid analysis of difficult matrices
Application of the flow-through enzyme immunoassay in a market orientated supply study for the evaluation of fumonisins in cornflakes
Evaluation of fumonisin contamination in cornflakes on the Belgian market by "flow-through" assay screening and LC-MS/MS analyses
A total of 205 cornflake samples collected in Belgian retail stores during 2003-2004 were surveyed for the natural occurrence of fumonisin B-1 (FB1), B-3 (FB3), and B-3 (FB3). These cornflake samples, originating from conventional as well as from organic production, were analyzed using an intralaboratory-validated LC-MS/MS method. Additionally, 90 cornflake samples were subjected to rapid screening using a flow-through enzyme immunoassay method to demonstrate the practicability of a screening test coupled to a validated confirmatory LC-MS/MS method for the management of food safety risks. FB1 concentrations ranged from not detected (nd) [LOD (FB1) = 20 mu g/kg] to 464 mu g/kg with mean and median concentrations of respectively 104 +/- 113 and 54 mu g/kg. For FB2 and FB3, the concentration ranges varied respectively from nd [LOD (FB2) = 7.5 mu g/kg] to 43 mu g/kg and from nd [LOD (FB3) = 12.5 mu g/kg] to 90 mu g/kg. Mean concentrations for FB2 and FB3 were respectively 12 +/- 8 and 21 +/- 15 mu g/kg, while the median concentration was 11 mu g/kg for FB2 and 19 mu g/kg for FB3. From the statistical tests (chi(2) and ANOVA model III), it could be concluded that the agricultural practice did not have any significant effect on the fumonisin concentrations but that the variation between different batches was significant (p < 0.0001)
A flow-through enzyme immunoassay for the screening of fumonisins in maize
The format of an existing flow-through enzyme immunoassay has been optimised for the detection of fumonisin B-1 (FB1) and B-2 (FB2) in maize. The visual detection limit i.e. the smallest mycotoxin concentration resulting in no colour development, is 1000 mug/kg. Assay validation was performed using samples spiked with respectively only FBI and a FB1 + FB2 mixture (ratio 1:1). Results showed that within-day and between-day coefficients of variation ranged from respectively 0.4-10.2% and 1.1-9.0%. Naturally contaminated samples were screened with the developed flow-through method and results were compared with fumonisin contamination values obtained by a validated HPLC method. The assay was demonstrated to be accurate and reliable giving no false compliant and only a low percentage of false non-compliant results. The described method offers a simple, rapid and cost-effective screening tool, thus contributing to a better consumers' health protection. (C) 2004 Elsevier B.V. All rights reserved
Mycotoxin contamination of conventional and organic cereal-based foodstuffs on the Belgian market
Development and application of analytical methods adapted to the determination of mycotoxins and environmental pollutants in some organic and home-produced foodstuffs
Mycotoxines dans les denrées alimentaires des filières conventionnelles et 'bio' : résultats d'une étude menée en Belgique sur des produits dérivés des céréales (Mycotoxin in conventional and organic foodstuffs : results on cereal-derived products on the Belgian market)
L’objectif de cette recherche est de dresser un état des lieux
de la contamination des denrées dérivées des céréales en
Belgique et d’étudier l’influence du mode de production
conventionnel versus biologique sur la teneur en
mycotoxines