In vitro propagation of khinjuk pistachio (Pistacia khinjuk stocks) through seedling apical shoot tip culture

An efficient method was developed for in vitro propagation of Pistacia khinjuk (khinjuk pistachio). Shoot cultures were established from aseptically germinated seedlings of khinjuk pistachio. Murashige and Skoog (MS) medium with Gamborg's vitamins containing 30 g P sucrose, 100 mg l(-1) I-ascorbic acid, 1 mg l(-1) BA and 7 g l(-1) agar resulted in multiple shoot initiation at the rate of 7.25 +/- 0.95 shoots per explant in 28 days of culture. Efficient rooting was achieved in MS medium supplemented with 0.5 mg l(-1) IBA. The in vitro raised plants were acclimatized in a growth room and transplanted to the field successfully. The method described will be useful for rapid multiplication of khinjuk pistachio for commercial exploitation

Studies on accumulation of Mn2+ by Nasturtium officinale in water by biosorption

In present study, Nasturtium officinale has been used as a plant. The plant has been collected from the campus of Dicle university. The study has been achieved in vitro. Air roots of the plant with the same number and morphology of leaf has been chosen. For biosorption of Mn2+ metal ions by Nasturtium officinale, the solutions were prepared as 0.1; 0.3; 0.5; 1; 2; 3; 4 and 5 ppm, respectively. Water samples has been prepared from 1000 ppm stock and put them to balon joje. After this process, the plants have been transferred to the erlens and wait for 24,48 and 72 h. The erlens have been tightly closed with parafilm. The general appearence of the plants have been observed at the beginning and after 24 h. The plants has been analyzed with atomic absorption spectrometry by receiving 2 mL of water samples for calculation of concentration. The A and B values have been found by drawing the calibration lines in UV. The wet weight has been measured with sensitive balance. The quantity of Mn2+ absorption of plants has been calculated with the dependence of the wet weight

The comparison studies on the proliferation of lateral sues of Vitis vinifera L.cv. Alphonse during different periods of the year at in vitro conditions

In this study, lateral buds of Vitis vinifera L.cv. Alphonse were used as material. During micropropagation, 2mg/l BAP (6-benzylaminopurine) and during rooting, 2mg/l NAA (proportional to-Naftalen Aseticacid) was added into the Murashige-Skoog (1) medium, which is used in culture studies. our aim is to compare proliferation capabilities of lateral buds of Alphonse grapevine in terms of various periods of year in in-vitro conditions. As a result, rooty seedling were obtained from lateral buds cultivated in yearly period of time,and it was determined that the most favourable and productive seedlings were those obtained from materials cultivated in the months November and December. It was also established that the success rate of seedlings obtained from materials cultivated in these months was about 80-85 %

The comparison studies on the proliferation of lateral buds of Vitis vinifera L.cv. alphonse during different periods of the year at in vitro conditions

In this study, lateral buds of Vitis vinifera L.cv. Alphonse were used as material. During micropropagation, 2mg/l BAP (6-benzylaminopurine) and during rooting, 2mg/l NAA (?-Naftalen Aseticacid) was added into the Murashige-Skoog (1) medium, which is used in culture studies. our aim is to compare proliferation capabilities of lateral buds of Alphonse grapevine in terms of various periods of year in in-vitro conditions. As a result, rooty seedling were obtained from lateral buds cultivated in yearly period of time, and it was determined that the most favourable and productive seedlings were those obtained from materials cultivated in the months November and December. It was also established that the success rate of seedlings obtained from materials cultivated in these months was about 80-85%

The impacts of different auxins on phenolic contents and the total antioxidant capacity of hypericum retusum aucher

The study was aimed to evaluate the effects of different auxins on phenolic contents and antioxidant potential of methanolic extract of Hypericum retusum Aucher (Clusiaceae) plantlets grown under in vitro conditions. After seed sterilisation and germination, shoot proliferations were performed. In this study, shoots were separately cultured in the medium containing BAP (0.5 mgl(-1)) combined with three different auxins (0.25 mgl(-1) IAA, NAA, IBA) and the highest number of shoots was obtained on medium supplemented with 0.5 mgl(-1) BAP. The highest total antioxidant capacity and phenolic contents were observed auxins. The methanol extracts of plantlets grown in vitro conditions showed the strongest free radical scavenging capacities at concentrations of 100 and 150 mu g/ml. It has been found that auxins and in vitro growth conditions have a significant effect on the total antioxidant capacity and phenolic contents