6,243 research outputs found
Monoamine oxidase-A modulates apoptotic cell death induced by staurosporine in human neuroblastoma cells
Monoamine oxidases (MAOs) are mitochondrial enzymes which control the levels of neurotransmitters in the brain and dietary amines in peripheral tissues via oxidative deamination. MAO has also been implicated in cell signalling. In this study, we describe the MAO-A isoform as functional in apoptosis induced by staurosporine (STS) in human dopaminergic neuroblastoma cells (SH-SY5Y). Increased levels of MAO-A activity were induced by STS, accompanied by increased MAO-A protein and activation of the initiator of the intrinsic pathway, caspase 9, and the executioner caspase 3. MAO-A mRNA levels were unaffected by STS, suggesting that changes in MAO-A protein are due to post-transcriptional events. Two unrelated MAO-A inhibitors reduced caspase activation. STS treatment resulted in sustained activation of the mitogen-activated protein kinase pathway enzymes extracellular regulated kinase, c-jun terminal kinase and p38, and depletion of the anti-apoptotic protein Bcl-2. These changes were significantly reversed by MAO inhibition. Production of reactive oxygen species was increased following STS exposure, which was blocked by both MAO inhibition and the antioxidant N-acetylcysteine. Therefore our data provide evidence that MAO-A, through its production of reactive oxygen species as a by-product of its catalytic activity on the mitochondrial surface, is recruited by the cell to enhance apoptotic signalling
New XMM-Newton analysis of three bright X-ray sources in M31 globular clusters, including a new black hole candidate
We present detailed analysis of three globular cluster X-ray sources in the
XMM-Newton extended survey of M31. The X-ray counterpart to the M31 globular
cluster Bo 45 (XBo 45) was observed with XMM-Newton on 2006 December 26. Its
combined pn+MOS 0.3--10 keV lightcurve exhibited a r.m.s variability of ~10%,
and its 0.3--7.0 keV emission spectrum was well described by an absorbed power
law with photon index 1.440.12. Its variability and emission is
characteristic of low mass X-ray binaries (LMXBs) in the low-hard state,
whether the accretor is a neutron star or black hole. Such behaviour is
typically observed at luminosities \la10% Eddington. However, XBo 45
exhibited this behaviour at an unabsorbed, 0.3--10 keV luminosity of
2.5 erg s, or{~140%} Eddington for a 1.4
neutron star accreting hydrogen. Hence, we identify XBo 45 as a new
candidate black hole LMXB. XBo 45 appears to have been consistently bright for
~30 years, consistent with theoretical prediction for a globular cluster black
hole binary formed via tidal capture. Bo 375 was observed in the 2007, January
2 XMM-Newton observation, and has a two-component spectrum that is typical for
a bright neutron star LMXB. Bo 135 was observed in the same field as Bo 45, and
could contain either a black hole or neutron star.Comment: Accepted by ApJ, 16 pages, 5 figures. This version includes the final
changes made at the request of the refere
Computable functions, quantum measurements, and quantum dynamics
We construct quantum mechanical observables and unitary operators which, if
implemented in physical systems as measurements and dynamical evolutions, would
contradict the Church-Turing thesis which lies at the foundation of computer
science. We conclude that either the Church-Turing thesis needs revision, or
that only restricted classes of observables may be realized, in principle, as
measurements, and that only restricted classes of unitary operators may be
realized, in principle, as dynamics.Comment: 4 pages, REVTE
Analysis of a Large Sample of Neutrino-Induced Muons with the ArgoNeuT Detector
ArgoNeuT, or Argon Neutrino Test, is a 170 liter liquid argon time projection
chamber designed to collect neutrino interactions from the NuMI beam at Fermi
National Accelerator Laboratory. ArgoNeuT operated in the NuMI low-energy beam
line directly upstream of the MINOS Near Detector from September 2009 to
February 2010, during which thousands of neutrino and antineutrino events were
collected. The MINOS Near Detector was used to measure muons downstream of
ArgoNeuT. Though ArgoNeuT is primarily an R&D project, the data collected
provide a unique opportunity to measure neutrino cross sections in the 0.1-10
GeV energy range. Fully reconstructing the muon from these interactions is
imperative for these measurements. This paper focuses on the complete kinematic
reconstruction of neutrino-induced through-going muons tracks. Analysis of this
high statistics sample of minimum ionizing tracks demonstrates the reliability
of the geometric and calorimetric reconstruction in the ArgoNeuT detector
The Structure and X-ray Recombination Emission of a Centrally Illuminated Accretion Disk Atmosphere and Corona
We model an accretion disk atmosphere and corona photoionized by a central
X-ray continuum source. We calculate the opacity and radiation transfer for an
array of disk radii, to obtain the two-dimensional structure of the disk and
its X-ray recombination emission. The atmospheric structure is insensitive to
the viscosity alpha. We find a feedback mechanism between the disk structure
and the central illumination, which expands the disk and increases the solid
angle subtended by the atmosphere. We model the disk of a neutron star X-ray
binary. We map the temperature, density, and ionization structure of the disk,
and we simulate the high resolution spectra observable with the Chandra and
XMM-Newton grating spectrometers. The X-ray emission lines from the disk
atmosphere are detectable, especially for high-inclination binary systems. The
grating observations of two classes of X-ray binaries already reveal important
spectral similarities with our models. The line spectrum is very sensitive to
the structure of each atmospheric layer, and it probes the heating mechanisms
in the disk. The model spectrum is dominated by double-peaked lines of H-like
and He-like ions, plus weak Fe L. Species with a broad range of ionization
levels coexist at each radius: from Fe XXVI in the hot corona, to C VI at the
base of the atmosphere. The choice of stable solutions affects the spectrum,
since a thermal instability is present in the regime where the X-ray
recombination emission is most intense.Comment: 32 pages, incl. 26 figures, accepted for publication in Ap
Measurements of Sunyaev-Zel'dovich Effect Scaling Relations for Clusters of Galaxies
We present new measurements of the Sunyaev-Zel'dovich (SZ) effect from
clusters of galaxies using the Sunyaev-Zel'dovich Infrared Experiment (SuZIE
II). We combine these new measurements with previous cluster observations with
the SuZIE instrument to form a sample of 15 clusters of galaxies. For this
sample we calculate the central Comptonization, y, and the integrated SZ flux
decrement, S, for each of our clusters. We find that the integrated SZ flux is
a more robust observable derived from our measurements than the central
Comptonization due to inadequacies in the spatial modelling of the
intra-cluster gas with a standard Beta model. This is highlighted by comparing
our central Comptonization results with values calculated from measurements
using the BIMA and OVRO interferometers. On average, the SuZIE calculated
central Comptonizations are approximately 60% higher in the cooling flow
clusters than the interferometric values, compared to only approximately 12%
higher in the non-cooling flow clusters. We believe this discrepancy to be in
large part due to the spatial modelling of the intra-cluster gas. From our
cluster sample we construct y-T and S-T scaling relations. The y-T scaling
relation is inconsistent with what we would expect for self-similar clusters;
however this result is questionable because of the large systematic uncertainty
in the central Comptonization. The S-T scaling relation has a slope and
redshift evolution consistent with what we expect for self-similar clusters
with a characteristic density that scales with the mean density of the
universe. We rule out zero redshift evolution of the S-T relation at 90%
confidence.Comment: Accepted to Astrophysical Journal. 52 pages, 14 tables, 7 figures
;replaced to match ApJ accepted versio
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